2017 Fiscal Year Final Research Report
Development of revitalization therapy for stem cell depleted conditions
| Project/Area Number |
15H05002
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 幹細胞 / 放射線障害 |
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| Outline of Final Research Achievements |
Clinical sequelae of irradiation result in tissue devitalization, where wound healing capacity is impaired. Non-lethal irradiation at various doses (5-15 Gy) and frequencies (1-3 times on sequential days) was delivered to dorsal skin of nude mice.Wounds were locally injected with vehicle, cultured adipose-derived stem cells (ASCs), centrifuged fat tissue, or micronized cellular adipose matrix, monitoring therapeutic impact for up to 15 days. Radiation damage was dose-dependent. At highest dosage (15 Gy × 3), open wounds persisted on Day 15. However, wounds injected with cultured ASCs were nearly healed on Day 12, and those treated with injection of centrifuged fat or micronized connective tissue healed faster than untreated controls (p<0.05).Irradiation damage and tissue devitalization were dose-dependent, though fractionated protocols helped to reduce. ASCs and other fat-derived products harboring ASCs successfully revitalized irradiated tissues and accelerated wound healing.
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| Free Research Field |
形成外科
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