Biological synthesis of phosphate ions and its feedback mechanism of osteocyte

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H05010
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Hokkaido University
• Principal Investigator: AMIZUKA Norio 北海道大学, 歯学研究院, 教授 (30242431)
• Co-Investigator(Renkei-kenkyūsha): ODA Kimimitsu 新潟大学, 医歯学総合研究科, 教授 (10122681)
• Research Collaborator: HASEGAWA Tomoka ; HONGO Hiromi ; YAMAMOTO Tomomaya
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 骨細胞 / 石灰化 / FGF23 / ENPP1 / TNAP

Outline of Final Research Achievements

αKlotho-/-mice and kl/kl mice prevented the increment of serum phosphate level, when being fed with low-phosphate diets. Low-phosphate diets rescued the reduced mineralization of kl/kl bone but not αklotho-/-bone, indicating that bone mineralization seems to be regulated not only by serum concentration of phosphate, but also other mechanism. In a normal state, steoblasts being about to differentiate into osteocytes localized podoplanin and phsophorylated-ezrin along the cell membranes. ENPP1-/-mice and kl/kl mice prematurely demonstrated many osteoblasts featuring podoplanin and phosphorylated-ezrin. Therefore, the elevated concentration of serum phosphate and/or membrane transporters/enzymes serving for phosphate ion-supplement may be involved in osteocytic differentiation from osteoblasts.

Free Research Field

骨代謝学・細胞組織学