Dissection and reconstitution of ultradian genetic oscillators responsive to dynamic signaling inputs

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H05326
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Life / Health / Medical informatics
• Research Institution: Kyoto University
• Principal Investigator: Isomura Akihiro 京都大学, ウイルス・再生医科学研究所, 共同研究者 (70512466)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 遺伝子発現振動 / 光遺伝学 / 人工遺伝子回路 / 合成生物学 / 非線形動力学

Outline of Final Research Achievements

We studied dynamic responses of Hes1 genetic oscillators via optogenetic perturbation and single-cell imaging analysis. We found that Hes1 oscillators can decode dynamic signaling inputs at the single-cell level. We also succeeded in the visualization of single-cell phase response curves. Numerical simulations suggested that stochastic phase model is sufficient to explain synchronization processes of Hes1 oscillators upon external periodic perturbation.

Free Research Field

生物物理学