Establishment of bioluminescence sensor and imaging system for monitoring cell to cell communication

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H05488
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Analytical chemistry
• Research Institution: University of Fukui (2016); The University of Tokyo (2015)
• Principal Investigator: Hattori Mitsuru 福井大学, 学術研究院医学系部門, 助教 (20589858)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: ルシフェラーゼ / 生物発光 / イメージング / タンパク質

Outline of Final Research Achievements

We developed a new bioluminescence tool for monitoring protein dynamics in living cells including cell to cell communication, with newly constructed a microscope observation system. For tracing the temporal changing of cell activity rapidly, we choose the novel luciferase species that has sufficiently high luminescence intensity. We have already established an optical technique for monitoring protein-protein interactions in living cells based on complementation of split fragments of luciferase. In this case, the luciferase was also used as split fragments to monitor the protein that plays a key role in target phenomenon. We also developed a bioluminescence observation system based inverted fluorescence microscope, the set-up in which was with high sensitive and darkness condition. Finally, the luminescent probe in combination with the observation system monitored GPCR activities and the temporal correlation between cell and cell.

Free Research Field

生体計測