Interaction of membrane proteins on asymmetric lipid membrane toward creation of artificial translocon

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H05493
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Bio-related chemistry
• Research Institution: Kanagawa Institute of Industrial Sclence and Technology (2017); Kanagawa Academy of Science and Technology (2015-2016)
• Principal Investigator: Kamiya Koki 地方独立行政法人神奈川県立産業技術総合研究所, 人工細胞膜システムグループ, 研究員(任期有) (70612315)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: リポソーム / リン脂質非対称膜 / 膜タンパク質 / マイクロ流体デバイス / 人工細胞モデル

Outline of Final Research Achievements

In this study, I investigated interactions between asymmetric lipid membranes and biological molecules (peptides or membrane proteins). The asymmetric lipid vesicles were generated from a planar lipid bilayer by applying the pulsed jet flow. I achieved three items. Frist, I found that the reconstruction amounts of the membrane proteins into the lipid membranes changed by the lipid compositions, Second, I developed a device for sequentially generating various types of asymmetric lipid vesicles. Third, I developed a well-controlled cell or liposome-trapping device.

Free Research Field

生体関連化学、生物物理学