2016 Fiscal Year Final Research Report
Creation of an artificial hydrogenase enzyme with high activity using in vitro metal cofactor biosynthesis
| Project/Area Number |
15H06085
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Bio-related chemistry
|
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| Research Institution | Saitama University |
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Principal Investigator |
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| Project Period (FY) |
2015-08-28 – 2017-03-31
|
| Keywords | ヒドロゲナーゼ / 鉄 / コファクター / 生合成 / 酵素 |
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| Outline of Final Research Achievements |
[FeFe]-hydrogenase is an attractive metalloenzyme, because it can use molecular hydrogen, which is an eco-friendly energy carrier. To increase its activity, the metal cofactor as the active site was modified with artificial metal ions using apo-[FeFe]-hydrogenase and its three biosynthetic enzymes. As a result, the hydrogenases activity was not increased, whereas the other metal cofactor, iron-sulfur cluster might be decomposed. Therefore, for further study, iron-sulfur cluster biosynthetic proteins should be also mixed in the reaction mixture in order to avoid decomposition of the iron-sulfur cluster toward increasing the hydrogenase activity.
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| Free Research Field |
生物無機化学
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