2016 Fiscal Year Annual Research Report
食品機能性成分の効率的利用のためのナノ／マイクロキャリアの作製
| Project/Area Number |
15J00406
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| Research Institution | University of Tsukuba |
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Principal Investigator |
OSSAI EMMANUEL 筑波大学, 生命環境科学研究科, 特別研究員(DC2)
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| Project Period (FY) |
2015-04-24 – 2017-03-31
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| Keywords | Multiple emulsion / Microchannel / Lipid vesicles / Entrapment yield / Carriers / 5-fluorouracil / Metformin hydrochloride / Vesicle stability |
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| Outline of Annual Research Achievements |
In this year, the mechanisms of calcein leakage established in the previous year equally influenced the entrapment yields of 5-fluorouracil (5-FU) and metformin hydrochloride (MH), the antineoplatic and antihyperglycemic agents, respectively. For Tween 80 stabilized vesicle suspension, the entrapment yields of 5-FU and MH increased with decrease in the surfactant concentration, and then decreased at lower surfactant concentration. This trend was attributed to the combined effects of bilayer destabilization/solubilization and reversed-micellar transport. The entrapment yields of the drugs were found to be higher than those reported in the literature. However, the entrapment yield of calcein was remarkably higher than those of the drugs. The difference was explained in relation to their differing molecular weights and relative hydrophobicity. The vesicle suspension was equally found to be stable for about one month of storage at 4 °C. During storage, the entrapment yield decreased slightly and was considered to be due to minimal leakage of the drugs from the internal water phase of the vesicle to the continuous phase through the lipid bilayers. From these studies, the multiple emulsion and solvent evaporation integrated method offers great potentials for the formulation of lipid vesicles with controlled size and high entrapment yields of hydrophilic bioactive molecules, and would find industrial applications in pharmaceuticals, cosmetics, food, and other related fields.
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| Research Progress Status |
28年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
28年度が最終年度であるため、記入しない。
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Research Products
(5 results)
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[Journal Article] Preparation of lipid vesicles having suitable size for drug delivery with high entrapment of hydrophilic molecules using multiple emulsions2016
Author(s)
Ossai, E.C., Kuroiwa, T., Otsuka, Y., Motokui, Y., Wada, T., Isoda, T., Sato, S. and Ichikawa, S.
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Journal Title
Journal of Bioengineering & Biomedical Science
Volume: 7 (213)
Pages: 1 - 5
DOI
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Presentation] Mechanism of surfactant effect on the vesicle preparation using multiple emulsions2016
Author(s)
Ossai, E.C., Terasawa, J., Kanazawa, A., Kuroiwa, T., Sato, S. and Ichikawa, S.
Organizer
17th Annual Meeting, Japan Society for Food Engineering
Place of Presentation
Tokyo University of Marine Science and Technology, Shinagawa, Tokyo
Year and Date
2016-08-04 – 2016-08-05
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[Presentation] Efficient entrapment of hydrophilic molecule in lipid vesicles prepared by multiple emulsion method: Effect of surfactant type on the entrapment yield2016
Author(s)
Ossai, E.C., Terasawa, J., Horikoshi, K., Kuroiwa, T., Kanazawa, A., Sato, and S. Ichikawa, S.
Organizer
The 10th Conference of Aseanian Membrane Society (AMS 10)
Place of Presentation
Nara Kasugano International Forum IRAKA, Nara, Japan.
Year and Date
2016-07-26 – 2016-07-29
Int'l Joint Research
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[Presentation] Efficient entrapment of water-soluble molecule in lipid vesicles prepared by multiple emulsion method: Consideration on the mechanism of surfactant effect on the entrapment yield2016
Author(s)
Ossai, E.C., Terasawa, J., Kanazawa, A., Kuroiwa, T. and Ichikawa, S.
Organizer
The Society of Separation Process Engineers Japan
Place of Presentation
Nihon University, Funabashi, Chiba, Japan.
Year and Date
2016-05-27 – 2016-05-28
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