Development of ultra-high-amylose rice starch of BEs triple mutant and study of its properties for utilization

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K00779
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Niigata University
• Principal Investigator: Itoh Kimiko 新潟大学, 自然科学系, 教授 (10281007)
• Co-Investigator(Kenkyū-buntansha): 花城 勲 鹿児島大学, 農水産獣医学域農学系, 准教授 (30336325)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: Branchin Enzyme / Starch Synthase / BE / rice / starch / GBSSI / amylose / amylopectin

Outline of Final Research Achievements

To develop starch with a novel property, a ΔBEs/Wxa line with the reduced expression of three starch branching enzymes was developed. The appearance of trait of the mature seeds was white and opaque, and floury. Round and small starch grain was observed in the cross section of the seeds. The X-ray diffraction pattern of the ΔBEs/Wxa starch showed B type pattern. The unit chain length distribution of the starch showed that short- and long- chain fractions were greatly reduced. The apparent amylose content was up to 65% maximum, calculated from weight distribution of the unit chains. The true amylose content was up to 47.7 % maximum. Since the ΔBEs/Wxa starch was fine grain, round, and floury, it is expected to be used as an ultrafine grain powder.

Free Research Field

応用糖質科学