Expoloration of temperature sensing and signaling mechanism targeting isoprene emission

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07484
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Forest science
• Research Institution: University of the Ryukyus
• Principal Investigator: OKU Hirosuke 琉球大学, 熱帯生物圏研究センター, 教授 (10177165)
• Co-Investigator(Kenkyū-buntansha): 稲福 征志 琉球大学, 熱帯生物圏研究センター, 助教 (90457458)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: イソプレン / 熱帯樹木 / 温度変化 / シグナリング

Outline of Final Research Achievements

Isoprene emitted from terrestrial vegetation plays an important role in tropospheric chemistry. In order to clarify the temperature dependent molecular regulation of isoprene emission, we carried out a comprehensive analysis of the RNA-seq data set by using correlation analysis, network constructions and co-expression analysis. Results revealed a very close association of isoprene emission and IspS gene to genes in the plant hormone signal transduction pathway and circadian clock elements. Transcript levels of 29 genes in these pathways were validated by qRT-PCR. The upstream promoter region of IspS was also cloned and several putative cis-regulatory elements identified were consistent with transcriptional regulation in response to light, heat, temperature, plant hormones and circadian rhythmicity

Free Research Field

脂質生化学