2017 Fiscal Year Final Research Report
Development of foliar spray-type biopesticide against soil-borne diseases
| Project/Area Number |
15K07811
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental agriculture(including landscape science)
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| Research Institution | Gifu University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 青枯病 / 萎凋病 / 生物防除 / 内生細菌 / 茎葉散布 |
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| Outline of Final Research Achievements |
The aim of this study was to develop a novel microbial pesticide which can control soil-borne diseases by foliar application. For this purpose, we recovered 83 isolates of endophytic Bacillus spp. from tomato plants. By the preliminary screening using a tomato seedling assay, we selected 7 isolates, which showed >60% protection against tomato bacterial wilt (TBW), as primary biocontrol candidates. Subsequently, we tested the biocontrol effect of foliar application of the candidate isolates in pot experiments. As a result, an isolate GEBT457 showed the highest and stable suppressive effect against TBW. Accordingly, the isolate GEBT457 was selected as a final candidate. In the field experiments, foliar spraying of the isolate GEBT457 suppressed wilt incidence by about 40-50%, suggesting that this isolate was effective even under the field conditions. The result of gene expression analysis indicated that the candidate isolate induced systemic resistance in tomato plants.
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| Free Research Field |
植物病理学
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