Study on elucidation of the pathophysiology of rare intractable diseases (reticular dysgenesis) with severe combined immunodeficiency and development of therapeutic methods

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11072
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: The University of Tokushima
• Principal Investigator: NOMA Takafumi 徳島大学, 大学院医歯薬学研究部(歯学系), 教授 (40189428)
• Co-Investigator(Kenkyū-buntansha): 谷村 綾子 徳島大学, 大学院医歯薬学研究部(歯学系), 助教 (10610199) ; 堀口 大吾 徳島大学, 大学院医歯薬学研究部(歯学系), 助教 (70304532)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 幹細胞 / ミトコンドリア / エネルギー代謝 / 血球細胞分化制御

Outline of Final Research Achievements

The inducible FOXP3 expression vector was transduced into T cell-derived Jurkat cells by several methodology. After selection with puromycin,47 clones were obtained. Although all clones were analyzed for FoxP3 inducibility, no clone effectively induced FOXP3 gene expression by addition of doxycycline. From the verification experiments, it was suggested that the expression efficiency of the full - length isoform (v1) of FOXP3 gene in Jurkat cells is considerably lower than that of the v2 isoform lacking exon 2, possibly due to the involvement of cell - specific stability.

Free Research Field

医歯薬学