Noninvasive detection of oral cancer and precancerous lesion using aberrant DNA methylation in gargle fluid samples

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K11297
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kagoshima University
• Principal Investigator: Hamada Tomofumi 鹿児島大学, 医歯学域附属病院, 講師 (00444894)
• Co-Investigator(Kenkyū-buntansha): 横山 勢也 鹿児島大学, 医歯学域医学系, 助教 (20569941) ; 杉浦 剛 鹿児島大学, 医歯学域歯学系, 教授 (40322292) ; 河野 憲司 大分大学, 医学部, 教授 (50214664)
• Co-Investigator(Renkei-kenkyūsha): Yamada Norisige 京都大学, 医学研究科, 研究員 (60625242)
• Research Collaborator: NAKAMURA Kodai 鹿児島大学, 附属病院, 医員
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 口腔癌 / がん検診 / DNAメチル化 / エピゲノム / 前癌病変 / うがい液 / 口腔扁平上皮癌

Outline of Final Research Achievements

The purpose of this study is to find oral precancerous lesion-specific aberrant DNA methylation, and to establish the screening tool for the early detection of oral cancer/precancer. Gargle fluid samples were obteined from patients with leukoplakia and healthy subjects. Using selected cancer-related genes, the sensitivity and specify for oral precancer detection is 98% and 94, respectively. This novel method using aberrant DNA methylation might be useful for noninvasive screening for the patients with oral cancer/precancer.

Free Research Field

口腔外科