Development of the chemical detectors and inhibitors for protein methylation toward chemical epigenomics

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K12761
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemical biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Sohtome Yoshihiro 国立研究開発法人理化学研究所, 袖岡有機合成化学研究室, 研究員 (50431888)
• Co-Investigator(Kenkyū-buntansha): 島津 忠広 国立研究開発法人理化学研究所, 主任研究員研究室等, 専任研究員 (10618771)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: エピゲノム / タンパク質メチル化 / 検出プローブ / 阻害剤 / プロテオーム / 翻訳後修飾

Outline of Final Research Achievements

Protein methylation is a reversible modification that varies dynamically in response to the environment. In order to identify methylated proteins, antibodies specific for methylated lysine residues are generally required. Since this methylated antibody is difficult to prepare, the methylome analysis has been relatively neglected as compared with other post-translational modification reactions. In this study, we aimed to 1) develop a methodology to detect protein methylation without using antibody, and to 2) explore protein inhibitors using the detection system.

Free Research Field

有機合成化学、ケミカルバイオロジー