2006 Fiscal Year Final Research Report Summary
Structure-function relationships of a yeast putative stretch-activated calcium channel
| Project/Area Number |
16370072
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Tokyo Gakugei University |
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Principal Investigator |
IIDA Hidetoshi Tokyo Gakugei University, Department of Education, Professor, 教育学部, 教授 (70124435)
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| Co-Investigator(Kenkyū-buntansha) |
IIDA Kazuko Tokyo Metropolitan Organization for Medical Research, The Tokyo Metropolitan Institute of Medical Science, Senior Researcher, 東京都臨床医学総合研究所, 主任研究員 (40151229)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Stretch-activated calcium channel / Calcium channel / Signal transduction / Saccharomyces cerevisiae / Arabidopsis thaliana / Calcium / Mid1 / Cch1 |
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| Research Abstract |
1) The amino acid residue Phe^<356> of the putative stretch-activated Ca^<2+> channel Mid1 was determined to be important for cell viability in yeast. Systematic substitution of various amino acids for Phe^<355> revealed that hydrophobicity of this residue is critical.
2) The Mid1 protein was found to be localized to the plasma membrane and endoplasmic reticulum membrane. Mid1 forms a oligomer via disulfide bonding.
3) A multicopy suppressor of the mid1 mutation was isolated and characterized. This multicopy suppressor is an N-terminally truncated form of the Spa2 protein important for polarized morphgenesis.
4) A gene encoding Cch1, which has been genetically suggested to function with Mid1 as a Ca^<2+> channel, was cloned. We have confirmed that Mid1 and Cch1 really cooperate to function as a Ca^<2+> channel.
5) Rice OsTpc1 that is homologous to yeast Cch1 was found to be sensitive to blockers of L-type voltage-gated calcium channels. In addition, The Arabidopsis Mca1 protein, which can complement the lethality of the yeast mid1 mutant, was isolated and characterized. Mca1 is crucial for Ca^<2+> influx and touch sensing in primary roots.
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Research Products
(18 results)
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[Journal Article] Arabidopsis plasma membrane protein crucial for Ca^<2+> influx and touch sensing in roots2007
Author(s)
Nakagawa, Y., Katagiri, T., Shinozaki, K., Qi, Z., Tatsumi, H., Furuichi, T., Kishigami, A., Sokabe, M., Kojima, I., Sato, S., Kato, T., Tabata, S., Iida, K., Terashima, A., Nakano, M., Ikeda, M., Yamanaka, T., Iida.H.
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Journal Title
Proc. Natl. Acad. Sci. USA 104
Pages: 3639-3644
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Arabidopsis plasma membrane protein crucial for Ca^<2+> influx and touch sensing in roots.2007
Author(s)
Nakagawa, Y., Katagiri, T., Shinozaki, K., Qi, Z., Tatsumi, H., Furuichi, T., Kishigami, A., Sokabe, M., Kojima, I., Sato, S., Kato, T., Tabata, S., Iida, K., Terashima, A., Nakano, M., Ikeda, M., Yamanaka, T., Iida H.
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Journal Title
Proc.Natl.Acad.Sci.USA 104
Pages: 3639-3644
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of functional domains of Mid1, a stretch-activated channel component, necessary for localization to the plasma membrane and Ca^<2+> permeation2005
Author(s)
Ozeki-Miyawaki, C., Moriya, Y., Tatsumi, H., Iida, H., Sokabe, M.
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Journal Title
Exp. Cell Res. 311
Pages: 84-95
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Identification of functional domains of Mid1, stretch-activated channel component, necessary for localization to the plasma membrane and Ca^<2+> permeation.2005
Author(s)
Ozeki-Miyawaki, C., Moriya, Y., Tatsumi, H., Iida H., Sokabe, M.
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Journal Title
Exp.Cell Res. 311
Pages: 84-95
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Functional analysis of a rice putative voltage-dependent Ca2+ channel, OsTPC1, expressed in yeast cells lacking its homologous gene CCH12004
Author(s)
Hashimoto K., Saito, M., Matsuoka, H., Iida, K., Iida, H.
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Journal Title
Plant Cell Physiol. 45
Pages: 496-500
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Functional analysis of a rice putative voltage-dependent Ca^<2+> channel, OsTPC1, expressed in yeast cells lacking its homologous gene CCH1.2004
Author(s)
Hashimoto, K., Saito, M., Matsuoka, H., Iida, K., Iida H.
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Journal Title
Plant Cell Physiol. 45
Pages: 496-500
Description
「研究成果報告書概要(欧文)」より
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