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2007 Fiscal Year Final Research Report Summary

Investigation of the roles of arachidonic acid metabolite at the process of placenta discharge at delivery of cattle and development of new delivery induction method

Research Project

Project/Area Number 16380182
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionNational Agricultural Research Organization

Principal Investigator

KAMADA Hachiro  National Agricultural Research Organization, Molecular Nutrition Research Team, Senior Researcher (70360443)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Masato  National Agriculture Research Center for Hokkaido region, Research Team for Dairy Production using Regional Feed Resources, Senior Researcher (50450325)
MURAI Masaru  National Agriculture Research Center for Hokkaido region, Research Team for Dairy Production using Regional Feed Resources, Team Reader (00414748)
UEDA Yasuko  National Agriculture Research Center for Hokkaido region, Intensive Grazing Research Team, Chief Researcher (30370604)
OHSHITA Tomoko  National Agriculture Research Center for Hokkaido region, Research Team for Dairy Production using Regional Feed Resource, Chief Researcher (40391479)
Project Period (FY) 2004 – 2007
KeywordsPlacenta separation / Oxoarachidonic acid / Matrix metalloproteinase / Lipoxygenase
Research Abstract

As a time of delivery can not be correctly expected, the attendance in the midnight delivery is unavoidable and hard labor for stock raising farmers. The delivery-control technique to alleviate mental and physical strain on the manager are required. The fetal delivery serving as the first stage of delivery is well understood; however, researches are not in progress regarding the release of unneeded placenta serving as the second stage, and the mechanism which induces the placental release has not been known yet. In this study, identification of signal for placenta release and its practical application for delivery induction method were investigated. It is known that a kind of matrix metalloproteinases (MMP) likely relates to placenta release. So we tried to search for the activator of placental MMP using cultured bovine fibroblast derived from placenta. First, we forcused on the metabolites of arachidonic acid (Ara) because those play important roles at the process of delivery. Cyclooxygenase metabolites of Ara (prostanoid) had no activity on MMP activation; however, one of lipoxygenase metabolites showed a strong activity on MMP activation. Delivery induction using prostaglandin (PG) frequently results in retained placenta. However, we succeeded in placenta release of those cows by injection of lipoxygenase metabolite of Ara. And the zymographic pattern of MMP activity of placenta in this case was same as that in normal delivery. While a large amount of proMMP (inactive form) accumulation in placenta was observed in the cases of PG-induced delivery and naturally occoured retained placenta, which suggested the failure of MMP activation. These results indicated that lipoxygenase metabolite of Ara is a signal for MMP activation and placenta release at a delivery. We could develope a new method of delivery induction without retained placenta.

  • Research Products

    (50 results)

All 2009 2008 2007 2006 2005 2004 Other

All Journal Article (25 results) (of which Peer Reviewed: 11 results) Presentation (19 results) Book (3 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] 妊娠牛の血漿及び胎子胎盤中のマトリックスメタロプロテアーゼ活性変化2007

    • Author(s)
      鎌田八郎, 上田靖子, 村井勝
    • Journal Title

      平成18年度研究成果情報

      Pages: 144-145

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] アラキドン酸は胎子胎盤由来繊維芽細胞のマトリックスメタロプロテアーゼを活性化する2007

    • Author(s)
      鎌田八郎, 上田靖子, 村井勝
    • Journal Title

      平成18年度研究成果情報

      Pages: 146-147

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Selenium supplementation to colostrums increases immunoglobulin Gabsorption by newborn calves.2007

    • Author(s)
      H. Kamada, I. Nonaka, Y. Ueda, M. Murai
    • Journal Title

      Journal of Dairy Science 90(12)

      Pages: 5665-5670

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Effects of selenium-rich yeast supplementation on the luteal function of postpartum cows.2007

    • Author(s)
      H. Kamada, Y. Ueda, T. Mitani, M. Miyaji, K. Nakada, T. Yasui, M. Murai
    • Journal Title

      Book of Abstracts of the 58th Annual Meeting of the European Association for Animal Production.

      Pages: 181

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Effects of intramuscular fat deposition on the beef traits of Japanese Black steers.2007

    • Author(s)
      Y. Ueda, A. Watanabe, M. Higuchi, H. Shingu, S. Kushibiki, M. Shinoda
    • Journal Title

      Animal Science Journal

      Pages: 189-194

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Changes of matrix metalloproteinases of blood plasma and fetal placenta of pregnat cows.2007

    • Author(s)
      H. Kamada, Y. Ueda, M. Murai
    • Journal Title

      Seikajouhou

      Pages: 144-145

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Arachidonic acid activates matrix metalloproteinase of placental fibroblasts.2007

    • Author(s)
      H. Kamada, Y. Ueda, M. Murai
    • Journal Title

      Seikajouhou

      Pages: 146-147

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Selenium supplementation to colostrums increases immunoglobulin G absorption by new born calves.2007

    • Author(s)
      H. Kamada, I. Nonaka, Y. Ueda, M. Murai
    • Journal Title

      Journal of Dairy Science No. 90

      Pages: 5665-5670

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Method of enhancing immuno- globulin G absorption efficiency by selenium addition to colostrum.2007

    • Author(s)
      H. Kamada
    • Journal Title

      Journal of Clinical Veterinary Medicine. No. 2007- 1

      Pages: 20-23

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of selenium-rich yeast supplementation on the luteal function of postpartum cows.2007

    • Author(s)
      H. Kamada, Y. Ueda, T. Mitani, M. Miyaji, K. Nakada, T. Yasui, M. Murai
    • Journal Title

      Book of Abstracts of the 58th Annual Meeting of the European Association for Animal Production

      Pages: 181

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of intramuscular fat d eposition on the beef traits of Japanese Black steers.2007

    • Author(s)
      Y. Ueda, A. Watanabe, M. Higuchi, H. Shingu, S. Kushibiki, M. Shinoda
    • Journal Title

      Animal Science Journal No78

      Pages: 189-194

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Selenium increases immunoglobulin G absorption by the intest-inal pinocytosis of newborn calves.2006

    • Author(s)
      H. Kamada, I. Nonaka, Y. Ueda, M. Murai
    • Journal Title

      Book of Abstracts of the 57th Annual Meeting of the European Association for Animal Production.

      Pages: 120

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] 初乳への無機セレンの添加は仔牛への免疫グロブリン移行を高める2006

    • Author(s)
      鎌田八郎, 野中最子, 上田靖子, 村井勝
    • Journal Title

      平成17年度研究成果情報

      Pages: 154-155

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] H. Kamada, I. Nonaka, Y. Ueda M. Murai2006

    • Author(s)
      H. Kamada
    • Journal Title

      Seleniu mincreases immunoglobulin G absorption by the int estinal pinocytosis of newborn calves. Book of Abstracts of the 57th Annual Meeting of the European Association for Animal Production

      Pages: 120

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Supple mentation of selenium increase IgG absorption.2006

    • Author(s)
      H. Kamada, I. Nonaka, Y. Ueda, M. Murai
    • Journal Title

      Seikajouhou

      Pages: 154-155

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Arachidonic acid activates the matrix metalloproteinase of placental fibroblast cells.2005

    • Author(s)
      H. Kamada, I. Nonaka, Y. Ueda, M. Murai
    • Journal Title

      Book of Abstracts of the 56th Annual Meeting of the European Association for Animal Production.

      Pages: 128

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] 泌乳初期の乳牛の採食量は第一胃充満度の物理的制約を受ける2005

    • Author(s)
      押尾秀一, 村井勝, 鎌田八郎, 上田靖子
    • Journal Title

      平成16年度研究成果情報

      Pages: 160-161

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Biochemical and transcriptomic analyses of two bovine skeletal muscles in Charolasis bulls divergently selected for muscle growth.2005

    • Author(s)
      K. Sudre, I. Cassari-Malek, A. Listrat, Y. Ueda, C. Leroux, C. Jurie, C. Auffray, G. Renand, P. Martin, J. Francois
    • Journal Title

      Meat Science

      Pages: 267-277

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Arachidonic acid activates the matrix metalloprotei nase of placental fibroblast cells.2005

    • Author(s)
      H. Kamada, I. Nonaka, Y. Ueda, M. Murai
    • Journal Title

      Book of Abstracts of the 56th Annual Meeting of the European Association for Animal Production

      Pages: 128

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Selenium deficiency of dairy cattle a nd its control.2005

    • Author(s)
      H. Kamada
    • Journal Title

      Fertilizer No. 101

      Pages: 122-125

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Francois, Biochemical and transcriptomic analyses of two bovine skeletal muscles in Charolasis bulls divergently selected for muscle growth.2005

    • Author(s)
      K. Sudre, I. Cassari-Malek, A Listrat, Y. Ueda, C. Leroux, C. Jurie, C. Auffray, G. Renand, P. Martin, J.
    • Journal Title

      Meat Science

      Pages: 267-277

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 「研究成果報告書概要(欧文)」より2005

    • Author(s)
      I. Cassari-Malek, Y. Ueda, C. Bernard, C. Jurie, K. Sudre, A. Listrat, I. Barnola, G. Gentes, C. Leroux, G. Renand, P. Martin, J. F. Hocquette
    • Journal Title

      Indicators of milk and beef quality

      Pages: 371-377

  • [Journal Article] 亜鉛給与は牛の輸送ストレス由来の白血球数や化学発光能の上昇を抑える2004

    • Author(s)
      野中最子, 中島一喜, 鎌田八郎, 柾木茂彦, 寺田文典, 高橋秀之
    • Journal Title

      平成15年度研究成果情報

      Pages: 17-18

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Feed int ake of dairy cattle is controlled by physical fullnes s of rumen2004

    • Author(s)
      S. Oshio, M. Murai, H. Kamada, Y. Ueda
    • Journal Title

      Seikajouhou

      Pages: 160-161

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Zinc supplementation to ca If decreases transport stress2004

    • Author(s)
      I. Nonaka, K. Nakashima, H. Kamada, S. Masaki, F. Terada, H. Takahashi
    • Journal Title

      Seikajouhou

      Pages: 17-18

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Effects of LOX-metabolites of arachidonicacid on MMP activation of bovine pi acental fibroblasts2009

    • Author(s)
      H. Kamada, M. Nakamura, M. Hayashi, H. Kadoka wa, K. Hodate, M. Murai
    • Organizer
      The 110th annual meeting of Japanese Society of, Animal Science
    • Place of Presentation
      Fujisawa
    • Year and Date
      20090327-20090329
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 胎盤由来線維芽細胞のMMP活性化に対するアラキドン酸リポキシゲナーゼ誘導体の影響2009

    • Author(s)
      鎌田八郎, 中村正斗, 林征幸, 角川博哉, 甫立京子, 村井勝
    • Organizer
      第104回日本畜産学会
    • Place of Presentation
      日本大学
    • Year and Date
      2009-03-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Effects of shortening of dry period in dairy cows on milk production and energy balance in early period.2008

    • Author(s)
      M. Nakamura, K. Nakajima, Y. Takahashi
    • Organizer
      The 109th annual meeting of Japanese Society of Animal Science.
    • Place of Presentation
      Mito
    • Year and Date
      20080327-20080328
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Relationship betwe en grazing time and flavor in milk.2008

    • Author(s)
      Y. Ueda, S. Asakuma, N. Akiyama, S. Miyaji, M. M urai, T. Matsumura, M. Shinoda
    • Organizer
      The 109th annual meeting of Japanese Society of Animal Science.
    • Place of Presentation
      Mito
    • Year and Date
      20080327-20080328
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 泌乳牛の放牧時間と乳中香気成分との関係2008

    • Author(s)
      上田靖子, 朝隈貞樹, 秋山典昭, 宮地慎, 村井勝, 松村哲夫, 篠田満
    • Organizer
      第109回日本畜産学会
    • Place of Presentation
      常磐大学
    • Year and Date
      2008-03-29
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 高泌乳牛における乾乳期短縮が泌乳前期の乳性産とエネルギー充足に及ぼす影響2008

    • Author(s)
      中村正斗, 中島恵一, 高橋雄治
    • Organizer
      第109回日本畜産学会
    • Place of Presentation
      常磐大学
    • Year and Date
      2008-03-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] hanges of L-carnithine of blood plasma and milk in dairy cows.2007

    • Author(s)
      Y. Ueda, M. Murai, H. Kamada, N. Akiyama
    • Organizer
      The 107th annual meeting of Japanese Society of Animal Science
    • Place of Presentation
      Tokyo
    • Year and Date
      20070327-20070329
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 泌乳牛における牛乳中及び血漿中L-カルニチン含量変化2007

    • Author(s)
      上田靖子, 村井勝, 鎌田八郎, 秋山典昭
    • Organizer
      第107回日本畜産学会
    • Place of Presentation
      麻布大学
    • Year and Date
      2007-03-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Characterization of milk flavor of grazing dairy cows.2006

    • Author(s)
      Y. Ueda, M. Murai, H. Kamada, N. Akiyama
    • Organizer
      The 61th annual meeting of Hokkaido Animal Science and Agriculture Society
    • Place of Presentation
      Sapporo
    • Year and Date
      2006-09-06
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] 放牧した牛の牛乳中から検出される香気成分の特徴と固相抽出法の検討2006

    • Author(s)
      上田靖子, 村井勝, 鎌田八郎, 秋山典昭
    • Organizer
      第61回北海道畜産学会
    • Place of Presentation
      北海道農業研究センター
    • Year and Date
      2006-09-05
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Development of silage processing for resource circulation-type animal production utilizing potato starch pulp.2005

    • Author(s)
      Y .Ueda, M. Murai, H. Kamada, Y. Cai, T. Oshita, F. Akiyama
    • Organizer
      The 14th European Biomass Conference and Exhibition.
    • Place of Presentation
      France
    • Year and Date
      20051017-20051021
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Selenium intakeand plasma level of dairy cattle using regional feed resources.2005

    • Author(s)
      H. Kamada, Y. Ueda, M. Murai
    • Organizer
      The 105th annual meeting of Japanese Society of Animal Science.
    • Place of Presentation
      Sapporo
    • Year and Date
      20050909-20050910
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] E ffects of protein supplementation to dairy cows gra zing all day.2005

    • Author(s)
      M. Murai, K. Umemura, M. Nakamura, M. Sakaguchi, H. Kamada, N. Watanabe, S. Sakanoue, Y. Ueda
    • Organizer
      105th annual meeting of Japanese Society of Animal Science
    • Place of Presentation
      Sapporo
    • Year and Date
      20050909-20050910
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Effects of inhibitors on the activation of matrix metalloproteinase of placental fibroblasts by arachidonic acid.2005

    • Author(s)
      H. Kamada, M. Murai, Y. Ueda
    • Organizer
      104th annual meeting of Japanese Society of Animal Science
    • Place of Presentation
      Tokyo
    • Year and Date
      20050327-20050329
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Development of silage processing for resource circulation-type animal production utilizing potato starch pulp.2005

    • Author(s)
      Y. Ueda, M. Murai, H. Kamada, Y. Cai, T. Ohshita, F. Akiyama
    • Organizer
      14th European Biomass Conference and Exhibition
    • Place of Presentation
      フランス
    • Year and Date
      2005-10-20
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 泌乳牛の昼夜放牧飼養における乳量、乳質に及ぼす蛋白質補助飼料の効果2005

    • Author(s)
      村井勝, 梅村和弘, 中村正斗, 坂口実, 鎌田八郎, 渡邊也恭, 坂上清一, 上田靖子
    • Organizer
      第105回日本畜産学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2005-09-10
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] 北海道での自給飼料多給時における乳牛のセレン摂取状況2005

    • Author(s)
      鎌田八郎, 上田靖子, 村井勝, 松井義貴
    • Organizer
      第105回日本畜産学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2005-09-09
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] アラキドン酸による胎盤由来繊維芽細胞の剥離作用に対する種々の阻害剤の影響2005

    • Author(s)
      鎌田八郎, 村井勝, 上田靖子
    • Organizer
      第104回日本畜産学会
    • Place of Presentation
      東京大学
    • Year and Date
      2005-03-27
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Supplementation of organic zincon immune function of calves transfered by a car.2004

    • Author(s)
      I. Nonaka, K. Nakashima, H. Kamada, S. Masaki, F. Terada, H. Takahashi
    • Organizer
      The 103th annual meeting of Japanese Society of Animal Science
    • Place of Presentation
      Tokyo
    • Year and Date
      20040329-20040331
    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 臨床獣医2007

    • Author(s)
      鎌田八郎
    • Total Pages
      4
    • Publisher
      緑書房
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 肥料2005

    • Author(s)
      鎌田八郎
    • Total Pages
      4
    • Publisher
      肥料協会新聞部
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Indicators of milk and beef quality2005

    • Author(s)
      I. Cassari-Malek, Y. Ueda, C. Bernard, C. Jurie, K. Sudre, A. Listrat, I. Barnola, G. Gentes, C. Leroux, G. Renand, P. Martin, J. F. Hocquette
    • Total Pages
      6
    • Publisher
      Wageningen Academic Publishers
    • Description
      「研究成果報告書概要(和文)」より
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://cryo.naro.affrc.go.jp/seika/h18/index.html

  • [Patent(Industrial Property Rights)] アラキドン酸誘導体等を用いた胎盤剥離方法Method for placental exfoliation using oxoarachidonic acid or the like.2008

    • Inventor(s)
      鎌田八郎
    • Industrial Property Rights Holder
      農業・食品産業技術総合研究機構
    • Industrial Property Number
      特許権特願2007-24603212/104260
    • Filing Date
      2008-04-16
    • Description
      「研究成果報告書概要(和文)」より
    • Overseas
  • [Patent(Industrial Property Rights)] アラキドン酸誘導体等を用いた胎盤剥離方法Method for placental exfoliation using oxoarachidonic acid or the like.2007

    • Inventor(s)
      鎌田八郎
    • Industrial Property Rights Holder
      農業・食品産業技術総合研究機構
    • Industrial Property Number
      特許権特願2007-24603212/104260
    • Filing Date
      2007-09-21
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-06-09  

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