2005 Fiscal Year Final Research Report Summary
Application of autotransfusion with -80℃ canine erythrocyte cryopreservation in veterinary medicine
| Project/Area Number |
16580262
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Clinical veterinary science
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| Research Institution | Yamaguchi University |
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Principal Investigator |
TAURA Yasuho Yamaguchi University, Faculty of Agriculture, Professor, 農学部, 教授 (80163153)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAICHI Munenazu Yamaguchi University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (60243630)
UNE Satoshi Yamaguchi University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (60294659)
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| Project Period (FY) |
2004 – 2005
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| Keywords | canine / -80℃ / autotransfusion / hydroxyethyl starch (HES) / erythrocyte / cryopreservation / veterinary medicine / cryoprotectant |
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| Research Abstract |
In this canine erythrocytes study, the effect of -196℃ or two step method(-196℃→80℃) cryopreservation was examined. Erythrocytes were mixed with cryoprotective solution containing hydroxyethyl starch (HES) or glycerol (GL). This mixture was filled into plastic bag made of vinyl chloride and frozen to -196℃ in liquid nitrogen. It was transferred to -80℃ deepfreezer and was preserved for 3 month. After thawing, erythrocytes were not washed, and autotransfused.
1)The canine erythrocytes frozen HES and GL at -196℃ could be autotransfused without side effects. The HES was considered to have a practical value rather than the GL.
2)HES was used a cryoprotective solution, the possibility of the plentiful cryopreservation of canine erythrocytes at two step method (-196℃→-80℃) was found out.
3)Canine erythrocytes were cryopreserved at -80℃ with HES for three months or more was found out. In recovery rate, osmotic fragility test and scanning electron microscopy, significant difference was not observed in each period. Therefore, -80℃ cryopreservation can preserve canine erythrocytes longer than liquefied preservation.
4)The canine erythrocytes frozen HES at two step method could be autotransfused without side effects.
This two step method that could be autotransfused, and was considered to have a practical value in veterinary medicine.
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Research Products
(6 results)
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[Journal Article] Incubation of canine red blood cells before freezing leads to an improuved saline stability and morphology after thawing when using a hydroxyethyl starch cryopresevation protocol.2005
Author(s)
Kim, H., Itamoto, K., Tanaka, S., Nakaichi, M., Sumida, S., Taura, Y.
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Journal Title
Low Temperature Medicine Vol.31
Pages: 33-35
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Application of phosphoenolpyruvate into canine red blood cell cryopreservation with hydroxyethyl starch.2005
Author(s)
Kim, H., Itamoto, K., Une S., Nakaichi, M., Taura, Y., Sumida, S.
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Journal Title
Cryo Letters. Vol.26
Pages: 1-6
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A comparative study of the effects of glycerol and hydroxyethyl starch in canine red blood cell cryopreservation.2004
Author(s)
Kim, H., Tanaka, S., Une, S., Nakaichi, M., Sumida, S., Taura, Y.
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Journal Title
Journal Veterinary Medical Science Vol.66
Pages: 1543-1547
Description
「研究成果報告書概要(欧文)」より
