Regulation of hematopoiesis and tumorigenesis by Bcl6 and AP-1 gene family.

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 16590947
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Kumamoto University
• Principal Investigator: OKADA Seiji Kumamoto University, Center for AIDS Research, Div.of Hematopoiesis, Professor, エイズ学研究センター, 教授 (50282455)
• Project Period (FY): 2004 – 2005
• Keywords: BCL6 / AP-1 / Dendritic cells / Hematopoietic stem cells

Research Abstract

The proto-oncogene bcl-6 and c-fos are ubiquitously expressed in various tissues, and play important role for the differentiation, proliferation, apoptosis and oncogeneisis. In this study, I focused on the function of bcl-6 and c-fos for the hematologic cells, using transgenic and gene deficient mice.
1.Function of Bcl-6 on erythroid differentiation
Bcl-6 detected in the TER119highCD71high subset of erythroblasts in the spleen of neonatal mice may be required to retain the erythroblasts in the cell proliferation stage.
2.Function of Bcl-6 on dendritic cell differentiation
Bcl-6 deficient mice showed decreased umber of dendritic cells in spleen. Especially CD8+ subfraction of dendriti cells was completely deficient.
3.Function of c-fos on B cell differentiation
Using H2-c-fos transgenic mice, we showed that c-Fos positively regulated blimp-1 expression and terminal differentiation of activated B cells.

Research Products

• [Journal Article] HIV-1 Nef interferes with M-CSF receptor signaling through Hck activation and inhibits M-CSF bioactivities (2005)
  • Author(s): Suzu S et al.
  • Journal Title: Blood 105・8 Pages: 3230-3237
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] BT-Ig-SF,a novelimmunoglobulin superfamily protein,functions as a cell adhesion molecule. (2005)
  • Author(s): Harada H et al.
  • Journal Title: Journal of Cellular Physiology 204・3 Pages: 919-926
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A role for C-Fos/AP-1 in B lymphocyte terminal differentiaton. (2005)
  • Author(s): Ohkubo Y et al.
  • Journal Title: The Journal of Immunology 174・12 Pages: 7703-7710
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Hematopoietic stem cell and marrowstromalcell for spinal cord injury in mice. (2005)
  • Author(s): Koda M et al.
  • Journal Title: NeuroReport 16・16 Pages: 1761-1767
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Hyperthermia suppressesthe cyotoxicity of NKcells via down-regulation of perforin-granzyme B expression. (2005)
  • Author(s): Koga T et al.
  • Journal Title: Biochemical and Biophysical Research Communications 337・4 Pages: 1319-1323
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Selective expansion and engraftment of human CD16^+ NK cells in NOD/Scid mice. (2005)
  • Author(s): Harada H et al.
  • Journal Title: Europcan Journal of Immunology 35・12 Pages: 3599-3609
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] HIV-1 Nef interferes with M-CSF receptor signaling through Hck activation and inhibits M-CSF bioactivities. (2005)
  • Author(s): Suzu S, Harada H, Matsumoto T, Okada S
  • Journal Title: Blood 105(8) Pages: 3230-3237
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] BT-Ig-SF, a novel immunoglobulin superfamily protein, functions as a cell adhesion molecule. (2005)
  • Author(s): Harada H, Suzu S, Hayashi Y, Okada S
  • Journal Title: J Cell Physiol 204(3) Pages: 919-926
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A role for c-Fos/Ap-1 in B lymphocyte terminal differentiation. (2005)
  • Author(s): Ohkubo Y, Arima M, Arguni E, Okada S, Yamashita K, Asari S, Obata S, Sakamoto A, Hatano M, O-Wang J, Ebara M, Saisho H, Tokuhisa T
  • Journal Title: J Immunol 174(12) Pages: 7703-7710
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Hematopoietic stem cell and marrow stromal cell for spinal cord injury in mice. (2005)
  • Author(s): Koda M, Okada S, Nakayama T, Koshizuka S, Kamada T, Nishio Y, Someya Y, Yoshinaga K, Okawa A, Mariya H, Yamazaki M
  • Journal Title: NeuroReport 16(16) Pages: 1761-1767
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Hyperthermia suppresses the cyotoxicity of NK cells via down-regulation of perforin-granzyme B expression. (2005)
  • Author(s): Koga T, Harada H, Shi TS, Okada S, Suico MA, Shuto T, Kai H
  • Journal Title: Biochem Biophys Res Comm 337(4) Pages: 1319-1323
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Selective expansion and engraftment of human CD 16^+ NK cells in NOD/Said mice. (2005)
  • Author(s): Harada H, Suzu S, Ito T, Okada S
  • Journal Title: Eur J Immunol 35(12) Pages: 3599-3609
  • Description: 「研究成果報告書概要(欧文)」より