2005 Fiscal Year Final Research Report Summary
Significance of E2-EPF in gastric cancer
| Project/Area Number |
16591355
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | The Jikei University School of Medicine |
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Principal Investigator |
TORIUMI Yasuo The Jikei University School of Medicine, Lecturer, 医学部, 講師 (20217590)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIBASHI Yoshio The Jikei University School of Medicine, Lecturer, 医学部, 講師 (00246373)
TAKADA Koji The Jikei University School of Medicine, Assistant Professor, 医学部, 助教授 (30179452)
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| Project Period (FY) |
2004 – 2005
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| Keywords | E2-EPF / Gastric Cancer / Ubiquitin |
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| Research Abstract |
1)RT-PCR
Gastric carcinoma cell lines (MKN1,MKN45,ECC10,GT3TkB) were examined for gene expression of E2-EPF using RT-PCR. mRNA expression of E2-EPF was observed in all of them.
2)Immunoblotting
Gastric carcinoma cell lines (MKN7,MKN45,IM95) and esophageal carcinoma cell line (KYSE 50) were analyzed by SDS-PAGE, followed by immunoblotting using an anti-E2-EPF polyclonal antibody of ab4504,and AP2120a. A single band of 24kDa was present in KYSE 50 and IM95 by using ab4504. But a single band of 48kDa was present in MKN7 and MKN45 by using ab4505. Various bands of muriti-ubiquitin chain conjugated with E2-EPF were ditected in each sample by using AP2120a. These result suggested that a double quantity body of E2-EPF might be present in gastric cancer.
3)Immunohistochemistry
Using paraffin-embedded specimens from patients with gastric cancer, E2-EPF protein was detected using an anti-E2-EPF polyclonal antibody of AP2120b using a dextran polymer conjugate two step visualization system. E2-EPF was enriched on the surface of nuclei in cancer cells. E2-EPF protein expression patterns within cancerous lesions were not associated with any of the following variables : TNM classification, stage, invasion into lymphatic or blood vessels, intramural metastasis, or histologic grades of differentiation.
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