Research on the expression and function of cytokine in the human endometrium during implantation period

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 16591672
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Oita University
• Principal Investigator: NASU Kaei Oita University, Faculty of Medicine, Assistant Professor (30274757)
• Co-Investigator(Kenkyū-buntansha): TAKAI Noriyuki Oita Univerkty, Faculty of Medicine, Instructor (50295185) ; KAWANO Yasushi Oita University, Faculty of Medicine, Assistant Professor (40274758)
• Project Period (FY): 2004 – 2007
• Keywords: Endometrium / Cytokine / chemokine / Tissue remodeling / Endometriosis / Leukocyte trafficking / Menstrual cycle / Inflammation

Research Abstract

The aim of our research project is to investigate the expression of chemokines in the human endometrium and their possible implications in the endometrium during the human reproductive processes. Our study a was conducted to identify the roles of chemokines in normal and pathological situations during the menstrual cycle and implantation. A number of chemokines mediate leukocyte traffic through their specific receptors in the human endometrium Although four subfamilies of chemokines have been described to date, CC-, CXC-, and CX_3C-chemokines are reported to be involved in endometrial leukocyte trafficking and function and are also involved in other phenomena. Chemokines play relevant roles in many physiologic and pathologic endometrial events, such as menstruation, decidualization, implantation, and the maintenance of early pregnancy. Knowledge about these roles of may provide insight into the mechanism underlying the recruitment and function of inflammatory cells during the human reproductive processes.

Research Products

• [Journal Article] Tumor necrosis factor-α regulates vascular endothelial growth factor secretion byhuman oviductal epithelial cells and stromal fibroblasts (2007)
  • Author(s): Nasu K, Itoh H, Yuge A, Nishida M, Kawano Y, Narahara H.
  • Journal Title: Fertil Steril 87 Pages: 220-222
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Application ofthe nuclear factor-κB inhibitor,BAY11-7085,for the treatment of endometriosis:an in vitro study (2007)
  • Author(s): Nasu K, Nishida M, Ueda T, Yuge A, Takai N, Narahara H.
  • Journal Title: Am J Physiol Endocrinol Metab 293 Pages: E16-E23
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Interleukin-13 stimulates the secretion ofvasculaz endothelial growth factor and solublefins-like tyrosine kinase-1 by human oviductal epithelial cells (2007)
  • Author(s): Nasu K, Itoh H, Yuge A, Kawano Y, Yoshimatsu J, Narahara H.
  • Journal Title: Eur J Obstet Gynecol Reprod Biol 133 Pages: 208-212
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Collagen contractility is enhanced in human endometriotic stromal cells:a possible mechanism involved in the pathogenesis of endometriosis-associated fibrosis (2007)
  • Author(s): Yhge A, Nasu K, Matsumoto H, Nishiida M, Narahara H.
  • Journal Title: Hum Reprod 22 Pages: 938-944
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Human oviductal epithelial cells express Toll-like receptor3 and repond to double-stranded RNA:Fallopian tube-specific mucoal immunity against viral infection (2007)
  • Author(s): Nasu K, Itoh H, Yuge A, Nishida M, Narahara H.
  • Journal Title: Hum Reprod 22 Pages: 356-361
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The role of apoptotic dysfunction in the pathogenesis of endometriosis (2007)
  • Author(s): Nasu K, Nishida M, Yuge A, Takai N, Narahara H.
  • Journal Title: Curr Women's Health Rev 3 Pages: 113-122
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Site-Specific innate immune system in the female genital tract (2007)
  • Author(s): Nasu K, Itoh H, Nishida M, Yuge A, Narahara H.
  • Journal Title: Curr Res Immunol 1 Pages: 171-191
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Regulation of contractility of cultured human endomerial stromal cells by tumor necrosis factor-α (2007)
  • Author(s): Yuge A, Nasu K, Tsusue H, Ikegami E, Nishida M, Matsumoto H, Narahara H.
  • Journal Title: Eur J Obstet Gynecol Reprod Biol (Epub ahead of print)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Tumor necrosis factor-a regulates vascular endothelial growth factor secretion by human oviductal epithelial cells and stromal fibroblasts (2007)
  • Author(s): Nasu, K, Itoh, H, Yuge, A, Nishida, M, Kawano, Y, Narahara, H
  • Journal Title: Fertil Steril 87 Pages: 220-222
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Application of the nuclear factor-KB inhibitor, BAY 11-7085, for the treatment of endometriosis : an in vitro study (2007)
  • Author(s): Nasu, K, Nishida, M, Ueda, T, Yuge, A, Takai, N, Narahara, H
  • Journal Title: Am J Physiol Endocrinol Metab 293 Pages: 16-23
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Interleukin-13 stimulates the secretion of vascular endothelial growth facto, and soluble fins-like tyrosine kinase-1 by human oviductal epithelial cells (2007)
  • Author(s): Nasu, K, Itoh, H, Yuge, A, Kawano, Y, Yoshimatsu, J, Narahara, H
  • Journal Title: Eur J Obstet Gynecol Reprod Biol 133 Pages: 208-212
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Collagen contractility is enhanced in human endometriotic stromal cells : a possible mechanism involved in the pathogenesis of endometriosis-associated fibrosis (2007)
  • Author(s): Yuge, A, Nasu, K, Matsumoto, H, Nishida, M, Narahara, H
  • Journal Title: Hum Reprod 22 Pages: 938-944
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Human oviductal epithelial cells express Toll-like receptor 3 and respond to double-stranded RNA : Fallopian tube-specific mucosal immunity against viral infection (2007)
  • Author(s): Nasu, K, Itoh, H, Yuge, A, Nishida, M, Narahara, H
  • Journal Title: Hum Reprod 22 Pages: 356-361
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The role of apoptotic dysfunction in the pathogenesis of endometriosis (2007)
  • Author(s): Nasu, K, Nishida, M, Yuge, A, Takai, N, Narahara, H
  • Journal Title: Curr Women's Health Rev 3 Pages: 113-122
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Site-Specific innate immune system in the female genital tract (2007)
  • Author(s): Nasu, K, Itoh, H, Nishida, M, Yuge, A, Narahara, H
  • Journal Title: Curr Res Immunol 1 Pages: 171-191
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Regulation of contractility of cultured human endometrial stromal cells by tumor necrosis factor-a. (2007)
  • Author(s): Yuge, A, Nasu, K, Tsusue, H, Ikegami, E, Nishida, M, Matsumoto, H, Narahara, H
  • Journal Title: Eur J Obstet Gynecol Reprod Biol(Epub ahead of print)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Regulation of proliferation,motility,and contractivity of cultured human endometrial stromal cells by transforming growth factor-β isoforms (2006)
  • Author(s): Nasu K, Nishida M, Masumoto H, Bing S, Inoue C, Kawano Y, Miyakawa L.
  • Journal Title: Fertil Steril 84Suppl2 Pages: 1114-1123
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] β-hydroxyisovaleryshikonin induces apoptosis and G0/G1 cell-cycle arrest of endometriotic tromal cells:a preliminary in vitro study (2006)
  • Author(s): Nishida M, Nasu K, Ueda T, Yuge A, Takai N, Narahara H.
  • Journal Title: Hum Reprod 21 Pages: 2850-2856
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Hypoxia regulaes vascular endothelial growth factor and soluble fms-like tyrosine kinase-1 secretion by human oviductal epithelial cells and stromal fibroblasts (2006)
  • Author(s): Itoh H, Nasu K, Matsumoto H, Kawano Y, Yoshimatsu J, Narahara H.
  • Journal Title: Fertil Steril 85Suppl1 Pages: 1097-1102
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Human oviductal stromal fibroblasts,but not oviductal epithelial cells,express Toll-like receptor4:the site-specific mucosal immunity of the human fallopian tube against bacterial infection (2006)
  • Author(s): Itoh H, Nasu K, Nishida M, Matsumoto H, Yuge A, Narahara H.
  • Journal Title: Am J Reprod Immunol 56 Pages: 91-101
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Insulin-like growth factor-I regulates vascular endothelial growth factor secretion byhuman oviductal epithelial cells and stromal fibroblasts (2006)
  • Author(s): Nasu K, Itoh H, Yuge A, Kawano Y, Narahara H.
  • Journal Title: J Soc Gynecol Investig 13 Pages: 368-371
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Interleukin-1β regulates vascular endothelial growth factor and soluble fms-like tyrosine kinase-1 secretion by human oviductal epithelial cells and stromal fibroblasts (2006)
  • Author(s): Nasu K, Itoh H, Yuge A, Kawno Y, Yoshimatsu J, Nazahaza H.
  • Journal Title: Gynecol Endocrinol 22 Pages: 495-500
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] β-hydroxyisovalerylshikonin induces apoptosis and GO/G1 cell-cycle arrest of endometriotic stromal cells : a preliminary in vitro study (2006)
  • Author(s): Nishida, M, Nasu, K, Ueda, T, Yuge, A, Takai, N, Narahara, H
  • Journal Title: Hum Reprod 21 Pages: 2850-2856
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Hypoxia regulates vascular endothelial growth factor and soluble fins-like tyrosine kinase-1 secretion by human oviductal epithelial cells and stromal fibroblasts (2006)
  • Author(s): Itoh, H Nasu, K, Matsumoto, H, Kawano, Y, Yoshimatsu, J, Narahara, H
  • Journal Title: Fertil Steril 85 Suppl 1 Pages: 1097-1102
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Human oviductal stromal fibroblasts, but not oviductal epithelial cells, express Toll-like receptor 4 : the site-specific mucosal immunity of the human fallopian tube against bacterial infection (2006)
  • Author(s): Itoh, H, Nasu, K, Nishida, M, Matsumoto, H, Yuge, A, Narahara, H
  • Journal Title: Am J Reprod Immunol 56 Pages: 91-101
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Insulin-like growth factor-I regulates vascular endothelial growth factor secretion by human oviductal epithelial cells and stromal fibroblasts (2006)
  • Author(s): Nasu, K, Itoh, H, Yuge, A, Kawano, Y, Narahara, H
  • Journal Title: J Soc Gynecol Investig 13 Pages: 368-371
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Interleukin-113 regulates vascular endothelial growth factor and soluble fins-like tyrosine kinase-1 secretion by human oviductal epithelial cells and stromal fibroblasts (2006)
  • Author(s): Nasu, K, Itoh, H, Yuge, A, Kawano, Y, Yoshimatsu, J, Narahara, H
  • Journal Title: Gynecol Endocrinol 22 Pages: 495-500
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Endometriotic cells are resistant to interferon-γ-induced cell growth inhibition and apoptosis:a possible mechanism involved in the pathogenesis of endometriosis (2005)
  • Author(s): Nishida M, Nasu K, Ueda T, Fukuda J, Takai N, Miyakawa I.
  • Journal Title: Mol Hum Reprod 11 Pages: 29-34
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Regulation of proliferation,motility,and contractility of human endometrial cells by platelet-derived growth factor (2005)
  • Author(s): Matsumoto H, Nasu K, Nishida M, Ito H, Bing S, Miyakawa I.
  • Journal Title: J Chin Endocrinal Metab 90 Pages: 3560-3567
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Bufalin induces apoptosis and the G0/G1 cell cycle arrest of endometriotic stromal cells:a promising agent for the treatment of endometriosis (2005)
  • Author(s): Nasu K, Nishida M, Ueda T, Takai N, Bing S, Narahara H, Miyakawa I.
  • Journal Title: Mol Hum Reprod 11 Pages: 817-823
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Endometriotic cells are resistant to interferon-y-induced cell growth inhibition and apoptosis : a possible mechanism involved in the pathogenesis of endometriosis (2005)
  • Author(s): Nishida, M, Nasu, K, Ueda, T, Fukuda, J, Takai, N, Miyakawa, I
  • Journal Title: Mol Hum Reprod 11 Pages: 29-34
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Regulation of proliferation, motility, and contractility of human endometrial stromal cells by platelet-derived growth factor (2005)
  • Author(s): Matsumoto, H, Nasu, K, Nishida, M, Ito, H, Bing, S, Miyakawa, I
  • Journal Title: J Clin Endocrinol Metab 90 Pages: 3560-3567
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Regulation of proliferation, motility, and contractivity of cultured human endometrial stromal cells by transforming growth factor-13 isoforms (2005)
  • Author(s): Nasu, K, Nishida, M, Matsumoto, H, Bing, S, Inoue, C, Kawano, Y, Miyakawa, I
  • Journal Title: Fertil Steril 84 Suppl 2 Pages: 1114-1123
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Bufalin induces apoptosis and the GO/G1 cell cycle arrest of endometriotic stromal cells : a promising agent for the treatment of endometriosis (2005)
  • Author(s): Nasu, K, Nishida, M, Ueda, T, Takai, N, Bing, S, Narahara, H, Miyakawa, I
  • Journal Title: Mol Hum Reprod 11 Pages: 817-823
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Down-regulation of interleukin-1 receptor type 1 expression causes the dysregulated expression of CXC chemoldnes in endometriotic stromal cells:a possible mechanism for the altered immunological funcions in endometriosis (2004)
  • Author(s): Nisluda M, Nasu K, Fukuda J, Kawano Y, Narahara H, Miyakawa I.
  • Journal Title: J Clin Endocrinol Metab 89 Pages: 5094-5100
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Down-regulation of interleukin-1 receptor type 1 expression causes the dysregulated expression of CXC chemokines in endometriotic stromal cells : a possible mechanism for the altered immunological functions in endometriosis (2004)
  • Author(s): Nishida, M, Nasu, K, Fukuda, J, Kawano, Y, Narahara, H, Miyakawa, I
  • Journal Title: J Clin Endocrinol Metab 89 Pages: 5094-5100
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Application of the nuclear factor-κB inhibitor,BAY 11-7085,for the treatment of endometriosis (2007)
  • Author(s): Nasu K, Nishida M, Yuge A, TakaiN, Narahara H.
  • Organizer: 23rd Annual Meeting ofthe European Society for HumanReproduction&Embryology
  • Place of Presentation: Lyon Convention Centre
  • Year and Date: 20070702-04
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Application of the nuclear factor-KB inhibitor, BAY 11-7085, for the treatment of endometriosis (2007)
  • Author(s): Nasu, K, Nishida, M, Yuge, A, Takai, N, Narahara, H
  • Organizer: 23rd Annual Meeting of the European Society for Human Reproduction & Embryology
  • Place of Presentation: Lyon, France
  • Year and Date: 20070602-04
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Site-specific mucosal immunity of upper female genital tract (2006)
  • Author(s): Nasu K.
  • Organizer: The 3rd Stage Suface Barrier Immunology Study Group 3rd Meeting
  • Place of Presentation: Miyazaki Phoenix Hotel
  • Year and Date: 20061027-28
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Site-specific mucosal immunity of upper female genital tract (2006)
  • Author(s): Nasu, K
  • Organizer: The 3rd Stage Surface Barrier Immunology Study Group 3rd Meeting
  • Place of Presentation: Miyazaki, Japan
  • Year and Date: 20061027-28
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Endometriotic cells are resistant to interferon-γ-induced cell growth inhibition and apoptosis (2005)
  • Author(s): Nasu K, Nishida M, Yuge A, Narahara H, Takai N.
  • Organizer: The XIXth Asian and Oceanic Congress of Obstetrics&Gynecology
  • Place of Presentation: COEX Convent ion&Exhibition Center
  • Year and Date: 20051001-05
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Endometriotic cells are resistant to interferon-y-induced cell growth inhibition and apoptosis (2005)
  • Author(s): Nasu, K, Nishida, M, Yuge, A, Takai, N, Narahara, H
  • Organizer: The XIXth Asian and Oceanic Congress of Obstetrics & Gynecology
  • Place of Presentation: Seoul, Korea
  • Year and Date: 20051001-05
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Cultured human endometrial epithelial cells produce thymus and activation-regulatedchemokine upon stimulation with hlterleukin-4 and interleukin-13 (2004)
  • Author(s): Nasu K, Sun B, Nishida M, Fukuda J, Narahara H, Miyakawa I.
  • Organizer: IX International Congress of Reproductive Immunology
  • Place of Presentation: 山のホテル
  • Year and Date: 20041011-15
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Cultured human endometrial epithelial cells produce thymus and activation-regulated chemokine upon stimulation with interleukin-4 and interleukin-13 (2004)
  • Author(s): Nasu, K, Sun, B, Nishida, M, Fukuda, J, Narahara, H, Miyakawa, I
  • Organizer: IX International Congress of Reproductive Immunology
  • Place of Presentation: Hakone, Japan
  • Year and Date: 20041011-15
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Hypoxia simultaneously inhibits endostatin production and stimulates vascular endothelial growth factor by cultured human endometrial stromal cells (2004)
  • Author(s): Nasu K, Nisluda M, Fukuda J, Sun B, Kawano Y, Miyakawa I.
  • Organizer: 37th Annual Meeting 37th Annual Meeting of theSociety for Study of Reproduction
  • Place of Presentation: Vancouver
  • Year and Date: 20040801-04
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Hypoxia simultaneously inhibits endostatin production and stimulates vascular endothelial growth factor by cultured human endometrial stromal cells (2004)
  • Author(s): Nasu, K, Nishida, M, Fukuda, J, Sun, B, Kawano, Y,-Miyakawa, I
  • Organizer: 37th Annual Meeting 37th Annual Meeting of the Society for Study of Reproduction
  • Place of Presentation: Vancouver, BC, Canada
  • Year and Date: 20040801-04
  • Description: 「研究成果報告書概要(欧文)」より