2020 Fiscal Year Annual Research Report
Project/Area Number |
16H06279
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Research Institution | National Institute of Genetics |
Principal Investigator |
小原 雄治 国立遺伝学研究所, 先端ゲノミクス推進センター, 特任教授
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Project Period (FY) |
2016 – 2021
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Keywords | 次世代DNAシーケンサー / 新規ゲノム配列決定 / リシーケンシング / エピゲノム / RNA-Seq / 1細胞解析 / メタゲノム解析 / スーパーコンピューター |
Outline of Annual Research Achievements |
①総括支援活動では、応募の機会増加の要望に応えるため今年度から年2回の公募とした。その結果、合計466の応募課題があり、207課題(44%)を採択した。コロナ禍の影響の中、昨年度より応募数は100件近く増加したが、支援内容の精査や支援経費の限度額設定等により、40%超の採択率を維持した。審査委員はすべてプラットフォーム外の専門家とし、評価が同程度の場合は若手、女性、少額科研費からの課題、初めての応募課題を優先した。支援課題は科研費生物系のほぼすべての分野に渡っており、支援の成果を含む論文として2020年度に161報が発表された。この中には「単核貪食細胞系の分化における遺伝子発現制御機構の包括的解明」(Nature Immunol.)、「キンギョの変異体の表現型多様性を作り上げる分子機構の理解」(Current Biol.)、「植物二次代謝経路のゲノム進化に学ぶ生合成デザイン」(Nature Comm.)など広い分野でのレベルの高い成果が含まれている。拡大班会議や情報解析講習会はオンサイト開催が必要なためにコロナ禍の中で見送ったが、WEB開催としたヒトゲノム研究倫理を考える会は5回開催し、各回約500名が参加した。 ②大規模配列解析拠点ネットワーク支援活動においては、最先端技術を支援に提供するとともに、染色体レベルの高精度ゲノム配列決定、シングルセル・空間遺伝子発現解析等の支援技術高度化を進めた。 ③高度情報解析支援ネットワーク活動では、支援から浮かび上がった課題を解決するソフトウェアの開発を進め、実際の課題への適用を進めた。2020年度には、超高速相同性検索ソフトウェアPZLASTの開発、Hi-Cデータを使ったゲノムアセンブリソフトウエア、ロングリードシミュレータPBSIM2の開発などを進め、②と合わせて24報の論文を発表した。
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Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
・応募の機会増加が懸案であったが、今年度から年2回の公募を行うことができた。前年度に比べ応募数は100件近く増加し、また支援希望内容も拡大しているが、経費上限の設定や支援内容精査などにより採択率を40%以上に維持することができた。科研費のほぼ全種目から課題採択できており、同程度の評価の場合は若手・女性・少額科研費からの課題及び新たな申請者を優先するなどの措置をとり、多様な課題への支援ができた。選考の過程では、コロナ禍のために審査委員会や支援候補課題のヒアリングなどすべてオンラインで行ない、結果的に双方の負担を減らすことができた。 ・コロナ禍のために試料送付の遅れや、支援拠点側の出勤抑制などが見られ、支援の遅れも生じたが、おおむね期間内に支援を完了できた。支援依頼者から要望が高かった拡大班会議と情報解析講習会はオンサイト開催が必要なために見送らざるを得なかったが、支援依頼者が自力で解析できるように解析パイプライン整備を進め支援に供した。 ・支援成果を含む論文発表は2020年度は161報と順調に増加した。支援技術の高度化のための活動も大規模DNAシーケンシング支援、高度情報解析支援共に進めることができ、高度化の論文は24報発表した。また情報解析講習会内容をまとめた書籍を刊行し、好評を得ている。 ・これらの活動を、当初計画通り国立遺伝学研究所を中核機関として、参加する班員が所属する主な機関を連携機関とする連携ネットワークを形成して進めることができた。
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Strategy for Future Research Activity |
最終年度になるので、次期体制につながるように以下の方策を進める ・最先端の技術の提供のために、支援拠点における支援技術の高度化を引き続き進める。ゲノム解析は技術進展のスピードが特に速く、2-3年で解析手法が様変わりするために、支援に確実に使われる場合は、最終年度であっても支援機器・技術の整備を工夫して進めていく。 ・支援課題の採択率は、現状は予算の制限から40%台にとどまっており、より多い課題の支援が求められる。現状では、各支援課題の支援経費の上限を設けて、これを超す分は経費負担を求める形にすることで、ようやくこの採択率になっている。解析内容を精査する効果はあるが、一方でゲノム解析においては一定規模以上で支援しないと意味のある結果が出ないことも多く、若手をはじめ、経費面で敷居が高くなるケースもある。そのためにも技術高度化により、より効果的な解析技術の整備やコスト削減を図っていく必要がある。他方で、ピーク研究支援を考慮して、よりタイムリーな支援のために、支援経費自己負担で随時応募可能とする制度を整備し、ピーク作りとすそ野拡大のバランスを図る。 ・大量データ解析の支援要望はますます増加していることから、解析パイプラインの整備とともに、情報解析講習会の開催を継続拡充する。また、ゲノム解析の感覚を研究者コミュニティに共有してもらうためにも、拡大班会議は重要であり、今後工夫して進めていきたい。また、実際のデータをもとに高度情報解析を行える稀な場であることから、若手の参画やon the job trainingなどを通じてインフォマティックス人材育成を継続する。また、支援で得られたデータの適切な共有/公開を進める。このために支援で得られたヒトゲノムデータはNBDCヒトゲノムDBへの格納が確実になるようにする。ヒトゲノム以外のDNA配列データはDDBJへまず格納し、そこからデータ回収してもらう方式により確実なデータ格納を進める。
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Research Products
(482 results)
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[Journal Article] MCM10 compensates for Myc-induced DNA replication stress in breast cancer stem-like cells2021
Author(s)
Takahiko Murayama, Yasuto Takeuchi, Kaoru Yamawaki, Toyoaki Natsume, Mengjiao Li, Rojas-Chaverra N. Marcela, Yutaka Suzuki, Masato T. Kanemaki, Koji Okamoto, Arinobu Tojo, Noriko Gotoh, et al.
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Journal Title
Cancer Science
Volume: 112
Pages: 1209-1224
DOI
Peer Reviewed / Open Access
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[Journal Article] Biological significance of KRAS mutant allele expression in ovarian endometriosis2021
Author(s)
Nozomi Yachida, Kosuke Yoshihara, Kazuaki Suda, Hirofumi Nakaoka, Haruka Ueda, Kentaro Sugino, Ryo Tamura, Tatsuya Ishiguro, Hiroaki Kase, Teiichi Motoyama, Takayuki Enomoto, et al.
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DOI
Peer Reviewed / Open Access
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[Journal Article] MeCP2 controls neural stem cell fate specification through miR-199a-mediated inhibition of BMP- Smad signaling2021
Author(s)
Nakashima H., Tsujimura K., Irie K., Imamura T., Trujillo CA., Ishizu M., Uesaka M., Pan M., Noguchi H., Okata K., Aoyagi K., Ando-Noda T., Okano H., Muotri AR., Nakashima K.
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Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Genomic profiling reveals heterogeneous populations of ductal carcinoma in situ of the breast2021
Author(s)
Satoi Nagasawa, Yuta Kuze, Ichiro Maeda, Yasuyuki Kojima, Ai Motoyoshi, Tatsuya Onishi, Hibiki Seino, Ayako Suzuki, Masahide Seki, Eisuke Inoue, Koichiro Tsugawa, Tomohiko Ohta, Yutaka Suzuki, et al.
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Journal Title
Communications Biology
Volume: 4
DOI
Peer Reviewed / Open Access
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[Journal Article] Aberrant splicing isoforms detected by full-length transcriptome sequencing as transcripts of potential neoantigens in non-small cell lung cancer2021
Author(s)
Miho Oka, Liu Xu, Toshihiro Suzuki, Toshiaki Yoshikawa, Hiromi Sakamoto, Hayato Uemura, Akiyasu C. Yoshizawa, Yutaka Suzuki, Tetsuya Nakatsura, Yasushi Ishihama, Ayako Suzuki, Masahide Seki
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Journal Title
Genome Biology
Volume: 22
DOI
Peer Reviewed / Open Access
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[Journal Article] Genetic analyses of a secondary poroma and trichoblastoma in a HRAS-mutated sebaceous nevus2021
Author(s)
Minowa, Tomoyuki ; Kamiya, Takafumi ; Hida, Tokimasa ; Okura, Masae ; Kato, Junji ; Idogawa, Masashi ; Tange, Shoichiro ; Hirano, Tomomi ; Tokino, Takashi ; Uhara, Hisashi,
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[Journal Article] Centriole and PCM cooperatively recruit CEP192 to spindle poles to promote bipolar spindle assembly2021
Author(s)
Takumi Chinen, Kaho Yamazaki, Kaho Hashimoto, Ken Fujii, Koki Watanabe, Yutaka Takeda, Shohei Yamamoto, Yuka Nozaki, Yuki Tsuchiya, Daisuke Takao, Daiju Kitagawa
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Journal Title
Journal of Cell Biology
Volume: 220
DOI
Peer Reviewed / Open Access
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[Journal Article] Serum Helicobacter pylori antibody reactivity in seven Asian countries using an automated latex aggregation turbidity assay2021
Author(s)
Junko Akada, Evariste Tshibangu-Kabamba, Vo Phuoc Tuan, Shusaku Kurogi, Yuichi Matsuo, Shamshul Ansari, Dalla Doohan, Bui Hoang Phuc, Phawinee Subsomwong, Yoshio Yamaoka, et al.
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Journal Title
Journal of Gastroenterology and Hepatology
DOI
Peer Reviewed / Int'l Joint Research
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[Journal Article] Genome editing reveals fitness effects of a gene for sexual dichromatism in Sulawesian fishes2021
Author(s)
Satoshi Ansai, Koji Mochida, Shingo Fujimoto, Daniel F. Mokodongan, Bayu Kreshna Adhitya Sumarto, Kawilarang W. A. Masengi, Atsushi Toyoda, Kiyoshi Naruse, Kazunori Yamahira, Jun Kitano, et al.
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Journal Title
Nature Communications
Volume: 12
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Chromosome-level genome assembly of Ophiorrhiza pumila reveals the evolution of camptothecin biosynthesis2021
Author(s)
Amit Rai, Hideki Hirakawa, Ryo Nakabayashi, Shinji Kikuchi, Koki Hayashi, Megha Rai, Hiroshi Tsugawa, Taiki Nakaya, Atsushi Toyoda, Shoko Hikosaka, Eiji Goto, Kazuki Saito, Mami Yamazaki, et al.
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Journal Title
Nature Communications
Volume: 12
DOI
Peer Reviewed / Open Access
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[Journal Article] A RUNX-CBF β-driven enhancer directs the Irf8 dose-dependent lineage choice between DCs and monocytes2021
Author(s)
Koichi Murakami, Haruka Sasaki, Akira Nishiyama, Daisuke Kurotaki, Wataru Kawase, Tatsuma Ban, Jun Nakabayashi, Satoko Kanzaki, Yoichi Sekita, Hideaki Nakajima, Keiko Ozato, Tohru Kimura, Tomohiko Tamura
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Journal Title
Nature Immunology
Volume: 22
Pages: 301-311
DOI
Peer Reviewed / Int'l Joint Research
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[Journal Article] Hamster PIWI proteins bind to piRNAs with stage-specific size variations during oocyte maturation2021
Author(s)
Kyoko Ishino, Hidetoshi Hasuwa, Jun Yoshimura, Yuka W Iwasaki, Hidenori Nishihara, Naomi M Seki, Yasubumi Sakakibara, Atsushi Toyoda, Takehiko Itoh, Mikiko C Siomi, Shinichi Morishita, Haruhiko Siomi, et al.
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Journal Title
Nucleic Acids Research
Volume: 49
Pages: 2700-2720
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Peer Reviewed / Open Access
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[Journal Article] Genome-wide analysis of long noncoding RNAs, 24-nt siRNAs, DNA methylation and H3K27me3 marks in Brassica rapa2021
Author(s)
Hasan Mehraj, Daniel J. Shea, Satoshi Takahashi, Naomi Miyaji, Ayasha Akter, Motoaki Seki, Elizabeth S. Dennis, Ryo Fujimoto, Kenji Osabe
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Journal Title
PLOS ONE
Volume: 16
Pages: e0242530
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Substantial anti-gout effect conferred by common and rare dysfunctional variants of URAT1/SLC22A122021
Author(s)
Yu Toyoda, Yusuke Kawamura, Akiyoshi Nakayama, Hirofumi Nakaoka, Toshihide Higashino, Seiko Shimizu, Ituro Inoue, Kimiyoshi Ichida, Hiroshi Suzuki, Tappei Takada, Hirotaka Matsuo, et al.
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[Journal Article] Vasculature-driven stem cell population coordinates tissue scaling in dynamic organs2021
Author(s)
R. Ichijo, M. Kabata, H. Kidoya, F. Muramatsu, R. Ishibashi, K. Abe, K. Tsutsui, H. Kubo, Y. Iizuka, S. Kitano, H. Miyachi, Y. Kubota, H. Fujiwara, A. Sada, T. Yamamoto, F. Toyoshima
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Journal Title
Science Advances
Volume: 7
Pages: eabd2575
DOI
Peer Reviewed / Open Access
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[Journal Article] Genetic switch in UV response of mimicry-related pale-yellow colors in Batesian mimic butterfly, Papilio polytes2021
Author(s)
Shinichi Yoda, Kousuke Sakakura, Tasuku Kitamura, Yûsuke KonDo, Kazuki Sato, Ryosuke Ohnuki, Itsuki Someya, Shinya Komata, Tetsuya Kojima, Shinya Yoshioka, Haruhiko Fujiwara
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Journal Title
Science Advances
Volume: 7
Pages: eabd6475
DOI
Peer Reviewed / Open Access
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[Journal Article] Helicobacter pylori type 4 secretion systems as gastroduodenal disease markers2021
Author(s)
Bui Hoang Phuc, Vo Phuoc Tuan, Ho Dang Quy Dung, Tran Thanh Binh, Pham Huu Tung, Tran Dinh Tri, Ngo Phuong Minh Thuan, Vu Van Khien, Takeshi Matsumoto, Yoshio Yamaoka, et al.
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Journal Title
Scientific Reports
Volume: 11
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Comparative Analysis of Patient-Matched PDOs Revealed a Reduction in OLFM4-Associated Clusters in Metastatic Lesions in Colorectal Cancer2021
Author(s)
Takuya Okamoto, David duVerle, Katsuyuki Yaginuma, Yasuko Natsume, Hitomi Yamanaka, Daisuke Kusama, Mayuko Fukuda, Mayuko Yamamoto, Yutaka Suzuki, Satoshi Nagayama, Ryoji Yao, et al.
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DOI
Peer Reviewed / Open Access
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[Journal Article] A GWAS approach to find SNPs associated with salt removal in rice leaf sheath2020
Author(s)
Sarin Neang, Marjorie de Ocampo, James A Egdane, John Damien Platten, Abdelbagi M Ismail, Masahide Seki, Yutaka Suzuki, Nicola Stephanie Skoulding, Mana Kano-Nakata, Akira Yamauchi, Shiro Mitsuya
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Journal Title
Annals of Botany
Volume: 126
Pages: 1193-1202
DOI
Peer Reviewed / Int'l Joint Research
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[Journal Article] Combination of KRAS and SMAD4 mutations in formalin-fixed paraffin-embedded tissues as a biomarker for pancreatic cancer2020
Author(s)
Takahiro Yokose, Minoru Kitago, Sachiko Matsuda, Yasushi Sasaki, Yohei Masugi, Yuki Nakamura, Masahiro Shinoda, Hiroshi Yagi, Takashi Tokino, Yuko Kitagawa, et al.
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Journal Title
Cancer Science
Volume: 111
Pages: 2174-2182
DOI
Peer Reviewed / Open Access
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[Journal Article] Pathogenesis of multiple pancreatic cancers involves multicentric carcinogenesis and intrapancreatic metastasis2020
Author(s)
Yusuke Fujita, Sachiko Matsuda, Yasushi Sasaki, Yohei Masugi, Minoru Kitago, Hiroshi Yagi, Yuta Abe, Masahiro Shinoda, Takashi Tokino, Michiie Sakamoto, Yuko Kitagawa
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Journal Title
Cancer Science
Volume: 111
Pages: 739-748
DOI
Peer Reviewed / Open Access
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[Journal Article] Setdla Insufficiency in Mice Attenuates Excitatory Synaptic Function and Recapitulates Schizophrenia-Related Behavioral Abnormalities2020
Author(s)
Kenichiro Nagahama, Kazuto Sakoori, Takaki Watanabe, Yusuke Kishi, Keita Kawaji, Michinori Koebis, Kazuki Nakao, Yukiko Gotoh, Atsu Aiba, Naofumi Uesaka, Masanobu Kano
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Journal Title
Cell Reports
Volume: 32
Pages: 108126
DOI
Peer Reviewed / Open Access
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[Journal Article] Multiomics Investigation Revealing the Characteristics of HIV-1-Infected Cells In Vivo2020
Author(s)
Hirofumi Aso, Shumpei Nagaoka, Eiryo Kawakami, Jumpei Ito, Saiful Islam, Benjy Jek Yang Tan, Shinji Nakaoka, Koichi Ashizaki, Katsuyuki Shiroguchi, Yutaka Suzuki, Yorifumi Satou, Yoshio Koyanagi, Kei Sato
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Journal Title
Cell Reports
Volume: 32
Pages: 107887
DOI
Peer Reviewed / Open Access
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[Journal Article] Immuno-Surgical Management of Pancreatic Cancer with Analysis of Cancer Exosomes2020
Author(s)
Yu Takeda, Shogo Kobayashi, Masatoshi Kitakaze, Daisaku Yamada, Hirofumi Akita, Ayumu Asai, Masamitsu Konno, Takahiro Arai, Yuichiro Doki, Hidetoshi Eguchi, Hideshi Ishii, et al.
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Journal Title
Cells
Volume: 9
Pages: 1645
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Ancient Jomon genome sequence analysis sheds light on migration patterns of early East Asian populations2020
Author(s)
Takashi Gakuhari, Shigeki Nakagome, Simon Rasmussen, Morten E. Allentoft, Takehiro Sato, Thorfinn Korneliussen, Atsushi Toyoda, Tsunehiko Hanihara, Eske Willerslev, Martin Sikora, Hiroki Oota, et al.
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Journal Title
Communications Biology
Volume: 3
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] The Genetic Basis of Morphological Diversity in Domesticated Goldfish2020
Author(s)
Tetsuo Kon, Yoshihiro Omori, Kentaro Fukuta, Hironori Wada, Masakatsu Watanabe, Zelin Chen, Miki Iwasaki, Atsushi Toyoda, Shawn M. Burgess, Hideki Noguchi, Takahisa Furukawa, et al.
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Journal Title
Current Biology
Volume: 30
Pages: 2260-2274.e6
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A genome database for a Japanese population of the larvacean Oikopleura dioica2020
Author(s)
Kai Wang, Ryo Tomura, Wei Chen, Miho Kiyooka, Hinako Ishizaki, Tomoyuki Aizu, Yohei Minakuchi, Masahide Seki, Yutaka Suzuki, Takehiko Itoh, Atsushi Toyoda, Hiroki Nishida, Takeshi A. Onuma, et al.
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Journal Title
Development, Growth & Differentiation
Volume: 62
Pages: 450-461
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Comparative genomic analyses illuminate the distinct evolution of megabats within Chiroptera2020
Author(s)
Masato Nikaido, Shinji Kondo, Zicong, Zhang, Jiaqi Wu, Hidenori Nishihara, Yoshihito Niimura, Yutaka Suzuki, Atsushi Toyoda, Asao Fujiyama, Sumio Sugano, Misako Yoneda, Chieko Kai, et al.
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DOI
Peer Reviewed / Open Access
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[Journal Article] Profibrotic function of pulmonary group 2 innate lymphoid cells is controlled by Regnase-12020
Author(s)
Yoshinari Nakatsuka, Ai Yaku, Tomohiro Handa, Alexis Vandenbon, Yuki Hikichi, Yasutaka Motomura, Yutaka Suzuki, Takuya Uehata, Takashi Mino, Tohru Tsujimura, Kazuyo Moro, Osamu Takeuchi, et al.
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Journal Title
European Respiratory Journal
Volume: 57
Pages: 2000018
DOI
Peer Reviewed
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[Journal Article] Corrigendum : Networking and Specificity-Changing DNA Methyltransferases in Helicobacter pylori2020
Author(s)
Hirokazu Yano, Md. Zobaidul Alam, Emiko Rimbara, Tomoko F. Shibata, Masaki Fukuyo, Yoshikazu Furuta, Atsushi Toyoda, Yutaka Suzuki, Sumio Sugano, Keigo Shibayama, Ichizo Kobayashi, et al.
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Journal Title
Frontiers in Microbiology
Volume: 11
DOI
Peer Reviewed / Open Access
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[Journal Article] Networking and Specificity-Changing DNA Methyltransferases in Helicobacter pylori2020
Author(s)
Hirokazu Yano, Md. Zobaidul Alam, Emiko Rimbara, Tomoko F. Shibata, Masaki Fukuyo, Yoshikazu Furuta, Atsushi Toyoda, Yutaka Suzuki, Sumio Sugano, Keigo Shibayama, Ichizo Kobayashi, et al.
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Journal Title
Frontiers in Microbiology
Volume: 11
DOI
Peer Reviewed / Open Access
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[Journal Article] Diploid Male Gametes Circumvent Hybrid Sterility Between Asian and African Rice Species2020
Author(s)
Daichi Kuniyoshi, Itaru Masuda, Yoshitaka Kanaoka, Yuki Shimazaki-Kishi, Yoshihiro Okamoto, Hideshi Yasui, Toshio Yamamoto, Kiyotaka Nagaki, Yoichiro Hoshino, Yohei Koide, Itsuro Takamure, Yuji Kishima
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Journal Title
Frontiers in Plant Science
Volume: 11
DOI
Peer Reviewed / Open Access
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[Journal Article] m6A demethylase ALKBH5 promotes proliferation of esophageal squamous cell carcinoma associated with poor prognosis2020
Author(s)
Yushi Nagaki, Satoru Motoyama, Tomokazu Yamaguchi, Midori Hoshizaki, Yusuke Sato, Teruki Sato, Yukio Koizumi, Akiyuki Wakita, Yuta Kawakita, Yoshihiro Minamiya, Keiji Kuba, et al.
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Journal Title
Genes to Cells
Volume: 25
Pages: 547-561
DOI
Peer Reviewed / Open Access
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[Journal Article] H3K27me3 demethylase UTX regulates the differentiation of a subset of bipolar cells in the mouse retina.2020
Author(s)
Umutoni, D., Iwagawa, T., Baba, Y., Tsuhako, A., Honda, H., Aihara, M, Watanabe, S.
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Journal Title
Genes to Cells
Volume: 25
Pages: 402-412
DOI
Peer Reviewed / Open Access
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[Journal Article] Long-read sequencing for non-small-cell lung cancer genomes2020
Author(s)
Yoshitaka Sakamoto, Liu Xu, Masahide Seki, Toshiyuki T. Yokoyama, Masahiro Kasahara, Yukie Kashima, Akihiro Ohashi, Yoko Shimada, Takashi Kohno, Yuichi Shiraishi, Ayako Suzuki, Yutaka Suzuki, et al.
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Journal Title
Genome Research
Volume: 30
Pages: 1243-1257
DOI
Peer Reviewed / Open Access
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[Journal Article] Mutation profiling of uterine cervical cancer patients treated with definitive radiotherapy2020
Author(s)
Yuya Yoshimoto, Yasushi Sasaki, Kazutoshi Murata, Shin-ei Noda, Yuhei Miyasaka, Junko Hamamoto, Mio Furuya, Junko Hirato, Yoshiyuki Suzuki, Tatsuya Ohno, Takashi Tokino, Takahiro Oike, Takashi Nakano
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Journal Title
Gynecologic Oncology
Volume: 159
Pages: 546-553
DOI
Peer Reviewed / Open Access
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[Journal Article] Convolutional Neural Network Can Recognize Drug Resistance of Single Cancer Cells2020
Author(s)
Kiminori Yanagisawa, Masayasu Toratani, Ayumu Asai, Masamitsu Konno, Hirohiko Niioka, Tsunekazu Mizushima, Taroh Satoh, Jun Miyake, Kazuhiko Ogawa, Andrea Vecchione, Yuichiro Doki, Hidetoshi Eguchi, Hideshi Ishii
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Journal Title
International Journal of Molecular Sciences
Volume: 21
Pages: 3166
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] COVID-19 Drug Discovery Using Intensive Approaches2020
Author(s)
Ayumu Asai, Masamitsu Konno, Miyuki Ozaki, Chihiro Otsuka, Andrea Vecchione, Takahiro Arai, Toru Kitagawa, Ken Ofusa, Masami Yabumoto, Takaaki Hirotsu, Masateru Taniguchi, Hidetoshi Eguchi, Yuichiro Doki, Hideshi Ishii
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Journal Title
International Journal of Molecular Sciences
Volume: 21
Pages: 2839
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Loss-of-function variants in NEK1 are associated with an increased risk of sporadic ALS in the Japanese population2020
Author(s)
Hiroya Naruse, Hiroyuki Ishiura, Jun Mitsui, Yuji Takahashi, Takashi Matsukawa, Jun Yoshimura, Koichiro Doi, Shinichi Morishita, Jun Goto, Tatsushi Toda, Shoji Tsuji
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Journal Title
Journal of Human Genetics
Volume: 66
Pages: 237-241
DOI
Peer Reviewed / Open Access
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[Journal Article] Targeted deep sequencing of DNA from multiple tissue types improves the diagnostic rate and reveals a highly diverse phenotype of mosaic neurofibromatosis type 22020
Author(s)
Yu Teranishi, Satoru Miyawaki, Hiroki Hongo, Shogo Dofuku, Atsushi Okano, Shunsaku Takayanagi, Takahiro Ota, Jun Yoshimura, Wei Qu, Jun Mitsui, Hirofumi Nakatomi, Shinichi Morishita, Shoji Tsuji, Nobuhito Saito
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Journal Title
Journal of Medical Genetics
DOI
Peer Reviewed
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[Journal Article] Differential dynamics and impacts of prophages and plasmids on the pangenome and virulence factor repertoires of Shiga toxin-producing Escherichia coli 0145 : H282020
Author(s)
Keiji Nakamura, Kazunori Murase, Mitsuhiko P. Sato, Atsushi Toyoda, Takehiko Itoh, Jacques Georges Mainil, Denis Piérard, Shuji Yoshino, Yasuhiro Gotoh, Yoshitoshi Ogura, Tetsuya Hayashi, et al.
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Journal Title
Microbial Genomics
Volume: 6
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A ubiquitous subcuticular bacterial symbiont of a coral predator, the crown-of-thorns starfish, in the Indo-Pacific2020
Author(s)
Naohisa Wada, Hideaki Yuasa, Rei Kajitani, Yasuhiro Gotoh, Yoshitoshi Ogura, Dai Yoshimura, Atsushi Toyoda, Sen-Lin Tang, Takehiko Itoh, Tetsuya Hayashi, Nina Yasuda, et al.
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Journal Title
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Reconstitution of the oocyte transcriptional network with transcription factors2020
Author(s)
Nobuhiko Hamazaki, Hirohisa Kyogoku, Hiromitsu Araki, Fumihito Miura, Chisako Horikawa, Norio Hamada, So Shimamoto, Orie Hikabe, Kinichi Nakashima, Tomoya S. Kitajima, Takashi Ito, Harry G. Leitch, Katsuhiko Hayashi
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Journal Title
Nature
Volume: 589
Pages: 264-269
DOI
Peer Reviewed / Int'l Joint Research
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[Journal Article] Transient IGF-1R inhibition combined with osimertinib eradicates AXL-low expressing EGFR mutated lung cancer2020
Author(s)
Rong Wang, Tadaaki Yamada, Kenji Kita, Hirokazu Taniguchi, Sachiko Arai, Koji Fukuda, Minoru Terashima, Hisanori Uehara, Xujun Han, Kunio Matsumoto, Wei Wang, Takeshi Suzuki, Seiji Yano, et al.
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Journal Title
Nature Communications
Volume: 11
DOI
Peer Reviewed / Open Access
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[Journal Article] Genome-wide association meta-analysis identifies GP2 gene risk variants for pancreatic cancer2020
Author(s)
Yingsong Lin, Masahiro Nakatochi, Yasuyuki Hosono, Hidemi Ito, Yoichiro Kamatani, Akihito Inoko, Hiromi Sakamoto, Fumihiko Matsuda, Michiaki Kubo, Shogo Kikuchi, Keitaro Matsuo, et al.
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Journal Title
Nature Communications
Volume: 11
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Design principles of a minimal auxin response system2020
Author(s)
Hirotaka Kato, Sumanth K. Mutte, Hidemasa Suzuki, Isidro Crespo, Shubhajit Das, Tatyana Radoeva, Mattia Fontana, D. Roeland Boer, Ryuichi Nishihama, Takayuki Kohchi, Dolf Weijers, et al.
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Journal Title
Nature Plants
Volume: 6
Pages: 473-482
DOI
Peer Reviewed / Int'l Joint Research
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[Journal Article] Aberrant mucosal immunoreaction to tonsillar microbiota in immunoglobulin A nephropathy2020
Author(s)
Hiroki Yamaguchi, Shin Goto, Nao Takahashi, Masafumi Tsuchida, Hirofumi Watanabe, Suguru Yamamoto, Yoshikatsu Kaneko, Koichi Higashi, Hiroshi Mori, Yukio Nakamura, Arata Horii, Ken Kurokawa, Ichiei Narita
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Journal Title
Nephrology Dialysis Transplantation
Volume: 36
Pages: 75-86
DOI
Peer Reviewed / Open Access
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[Journal Article] Splice-site mutations in KIF5A in the Japanese case series of amyotrophic lateral sclerosis2020
Author(s)
Hiroya Naruse, Hiroyuki Ishiura, Jun Mitsui, Yuji Takahashi, Takashi Matsukawa, Kaori Sakuishi, Kiyotaka Nakamagoe, Zenshi Miyake, Akira Tamaoka, Shinichi Morishita, Tatsushi Toda, Shoji Tsuji, et al.
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Journal Title
DOI
Peer Reviewed / Open Access
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[Journal Article] Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression2020
Author(s)
Yoichiro Ito, Goro Terai, Misa Ishigami, Noriko Hashiba, Yasuyuki Nakamura, Takahiro Bamba, Ryota Kumokita, Tomohisa Hasunuma, Kiyoshi Asai, Jun Ishii, Akihiko Kondo
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Journal Title
Nucleic Acids Research
Volume: 48
Pages: 13000-13012
DOI
Peer Reviewed / Open Access
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[Journal Article] Arabidopsis SMN2/HEN2, Encoding DEAD-Box RNA Helicase, Governs Proper Expression of the Resistance Gene SMN1/RPS6 and Is Involved in Dwarf, Autoimmune Phenotypes of mekk1 and mpk4 Mutants2020
Author(s)
Momoko Takagi, Naoki Iwamoto, Yuta Kubo, Takayuki Morimoto, Hiroki Takagi, Fuminori Takahashi, Takumi Nishiuchi, Keisuke Tanaka, Ken Shirasu, Kazuya Ichimura, et al.
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Journal Title
Plant and Cell Physiology
Volume: 61
Pages: 1507-1516
DOI
Peer Reviewed / Open Access
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[Journal Article] Non-pathogenic Escherichia coli acquires virulence by mutating a growth-essential LPS transporter2020
Author(s)
Kaito C, Yoshikai H, Wakamatsu A, Miyashita A, Matsumoto Y, Fujiyuki T, Kato M, Ogura Y, Hayashi T, Isogai T, Sekimizu K
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Journal Title
PLOS Pathogens
Volume: 16
Issue: 4
Pages: e1008469
DOI
Peer Reviewed / Open Access
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[Journal Article] HMGB1-mediated chromatin remodeling attenuatesI124gene expression for the protection from allergic contact dermatitis2020
Author(s)
Naoyuki Senda, Hideyuki Yanai, Sana Hibino, Lei Li, Yu Mizushima, Tomomitsu Miyagaki, Mai Saeki, Yusuke Kishi, Sho Hangai, Junko Nishio, Makoto Sugaya, Tadatsugu Taniguchi, Shinichi Sato
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Journal Title
Proceedings of the National Academy of Sciences
Volume: 118
Pages: e2022343118
DOI
Peer Reviewed
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[Journal Article] Fruit setting rewires central metabolism via gibberellin cascades2020
Author(s)
Yoshihito Shinozaki, Bertrand P. Beauvoit, Masaru Takahara, Shuhei Hao, Kentaro Ezura, Yutaka Suzuki, Satoshi Kuhara, Camille Bénard, Duyen Prodhomme, Yves Gibon, Hiroshi Ezura, Tohru Ariizumi, et al.
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Journal Title
Proceedings of the National Academy of Sciences
Volume: 117
Pages: 23970-23981
DOI
Peer Reviewed / Int'l Joint Research
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[Journal Article] Staphylococcus Agr virulence is critical for epidermal colonization and associates with atopic dermatitis development2020
Author(s)
Yuumi Nakamura, Hiroki Takahashi, Akiko Takaya, Yuzaburo Inoue, Yuki Katayama, Yoko Kusuya, Tatsuma Shoji, Sanami Takada, Hiroyuki Matsue, Gabriel Núñez, Naoki Shimojo, et al.
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Journal Title
Science Translational Medicine
Volume: 12
Pages: eaay4068
DOI
Peer Reviewed / Int'l Joint Research
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[Journal Article] ARID1A protein expression is retained in ovarian endometriosis with ARID1A loss-of-function mutations : implication for the two-hit hypothesis2020
Author(s)
Nozomi Yachida, Kosuke Yoshihara, Kazuaki Suda, Hirofumi Nakaoka, Haruka Ueda, Kentaro Sugino, Manako Yamaguchi, Yutaro Mori, Kaoru Yamawaki, Ituro Inoue, Takayuki Enomoto, et al.
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Journal Title
Scientific Reports
Volume: 10
DOI
Peer Reviewed / Open Access
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[Journal Article] A possible beneficial effect of Bacteroides on faecal lipopolysaccharide activity and cardiovascular diseases2020
Author(s)
Naofumi Yoshida, Tomoya Yamashita, Shigenobu Kishino, Hikaru Watanabe, Kengo Sasaki, Daisuke Sasaki, Tokiko Tabata, Yuta Sugiyama, Takuji Yamada, Jun Ogawa, Ken-ichi Hirata, et al.
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Journal Title
Scientific Reports
Volume: 10
DOI
Peer Reviewed / Open Access
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[Journal Article] Comprehensive investigation of RNF213 nonsynonymous variants associated with intracranial artery stenosis2020
Author(s)
Hiroki Hongo, Satoru Miyawaki, Hideaki Imai, Masahiro Shimizu, Shinichi Yagi, Jun Mitsui, Hiroyuki Ishiura, Jun Yoshimura, Koichiro Doi, Wei Qu, Shinichi Morishita, Shoji Tsuji, Nobuhito Saito, et al.
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Journal Title
Scientific Reports
Volume: 10
DOI
Peer Reviewed / Open Access
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[Journal Article] Targeted gene disruption of ATP synthases 6-1 and 6-2 in the mitochondrial genome of Arabidopsis thaliana by mitoTALENs2020
Author(s)
Shin-ichi Arimura, Hiroki Ayabe, Hajime Sugaya, Miki Okuno, Yoshiko Tamura, Yu Tsuruta, Yuta Watari, Shungo Yanase, Takaki Yamauchi, Takehiko Itoh, Atsushi Toyoda, Hideki Takanashi, Nobuhiro Tsutsumi
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Journal Title
The Plant Journal
Volume: 104
Pages: 1459-1471
DOI
Peer Reviewed
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[Journal Article] The formation of perinucleolar bodies is important for normal leaf development and requires the zinc-finger DNA-binding motif in Arabidopsis ASYMMETRIC LEAVES22020
Author(s)
Lilan Luo, Sayuri Ando, Yuki Sakamoto, Takanori Suzuki, Hiro Takahashi, Nanako Ishibashi, Shoko Kojima, Daisuke Kurihara, Tetsuya Higashiyama, Michiko Sasabe, Yasunori Machida, et al.
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Journal Title
The Plant Journal
Volume: 101
Pages: 1118-1134
DOI
Peer Reviewed / Open Access
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[Journal Article] Whole Genome Sequencing Reveals Virulence Potentials of Helicobacter pylori Strain KE21 Isolated from a Kenyan Patient with Gastric Signet Ring Cell Carcinoma2020
Author(s)
Catherine Mwangi, Stephen Njoroge, Evariste Tshibangu-Kabamba, Zahir Moloo, Allan Rajula, Smita Devani, Takashi Matsumoto, Kimang' a Nyerere, Samuel Kariuki, Gunturu Revathi, Yoshio Yamaoka
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Journal Title
Toxins
Volume: 12
Pages: 556
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Discovery of unique African Helicobacter pylori CagA-multimerization motif in the Dominican Republic2020
Author(s)
Takaaki Ono, Modesto Cruz, Hiroyuki Nagashima, Phawinee Subsomwong, Junko Akada, Takashi Matsumoto, Tomohisa Uchida, Rumiko Suzuki, Celso Hosking, José A Jiménez Abreu, Yoshio Yamaoka
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Journal Title
World Journal of Gastroenterology
Volume: 26
Pages: 7118-7130
DOI
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Protective effect of common and rare variants of URAT1/SLC22A12 on gout2021
Author(s)
Yu Toyoda, Yusuke Kawamura, Akiyoshi Nakayama, Hirofumi Nakaoka, Toshihide Higashino, Seiko Shimizu, Hiroshi Ooyama, Keito Morimoto, Naohiro Uchida, Ryuichiro Shigesawa, Kenji Takeuchi, Ituro Inoue, Kimiyoshi Ichida, Hiroshi Suzuki, Nariyoshi Shinomiya, Tappei Takada, Hirotaka Matsuo
Organizer
The 19th International Purine and Pyrimidine Symposium
Int'l Joint Research
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[Presentation] Genomic identification and characterization of sex-determining locus of Takifugu niphobles by a chromosome scale genome assembly2021
Author(s)
Kabir Ahammad, Risa Ieda, Daigaku Fujikawa, Shotaro Hirase, Takashi Koyama, Atsushi Toyota, Shigehiro Kuraku Sho Hosoya, Kiyoshi KikuchiKabir Ahammad, Risa Ieda, Daigaku Fujikawa, Shotaro Hirase, Takashi Koyama, Atsushi Toyota, Shigehiro Kuraku Sho Hosoya, Kiyoshi Kikuchi
Organizer
令和3年度日本水産学会春季大会
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[Presentation] Marpolbase : development of the genome and expression database for the liverwort Marchantia polymorphaMarpolbase : ゼニゴケのための遺伝子情報および発現量データベースの開発2021
Author(s)
谷澤 靖洋, 川村 昇吾, 矢倉 勝, 坂本 美佳, 望月 孝子, 山岡 尚平, 西浜 竜一, 大和 勝幸, 河内 孝之, Liu, Chang, Berger, Frédéric, 中村 保一
Organizer
第62回日本植物生理学会年会
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[Presentation] ユーストマの全ゲノム解読とDNAマーカー開発2021
Author(s)
川勝 恭子, 豊田 敦, 永野 惇, 川勝 泰二, 中野 善公, 今村 仁, 福田 直子, 久松 完, 山口 博康, 望月 孝子, 谷澤 靖洋, 坂本 美佳, 中村 保一
Organizer
日本育種学会第139回講演会
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[Presentation] Characteristics of Mosaic Neurofibromatosis type 2 Revealed by Deep Sequence for Multiple Tissue DNA2020
Author(s)
Yu Teranishi, Satoru Miyawaki, Hiroki Hongo, Shogo Dofuku, Atsushi Okano, Shunsaku Takayanagi, Takahiro Ota, Jun Yoshimura, Wei Qu, Jun Mitsui, Hirofumi Nakatomi, Shinichi Morishita, Shoji Tsuji, Nobuhito Saito
Organizer
2020 European NF Meeting
Int'l Joint Research
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[Presentation] YMPHOID-MYELOID CELL FATE DETERMINATION BY ENDORIBONUCLEASES REGNASE-1 AND -32020
Author(s)
Takuya, U., Yamada, S., Miyazaki, M., Giladi, A., Vandenbon, A., Amit, I., Kawamoto, H., Takeuchi, O.
Organizer
8th Annual Meeting ; International Cytokine & Interferon Society
Int'l Joint Research
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[Presentation] Single-cell analyses unravel unique features of plasma cell clones in POEMS syndrome2020
Author(s)
Yusuke Isshiki, Naoya Mimura, Motohiko Oshima, Kensuke Kayamori, Masahide Seki, Yurie Nagai, Shokichi Tsukamoto, Yusuke Takeda, Chikako Ohwada, Sonoko Misawa, Junichiro Ikeda, Satoshi Kuwabara, Yutaka Suzuki, Emiko Sakaida, Chiaki Nakaseko, Atsushi Iwama
Organizer
米国血液学会
Int'l Joint Research
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[Presentation] 空間的/シングルセル遺伝子発現情報統合解析が示す心臓内神経堤細胞の多様性と領域性2020
Author(s)
Akiyasu Iwase, Yasunobu Uchijima, Daiki Seya, Mayuko Kida, Hiroki Higashiyama, Kazuhiro Matsui, Akashi Taguchi, Shogo Yamamoto, Shiro Fukuda, Seitaro Nomura, Takahide Kohro, Chisa Shukunami, Masahide Seki, Yutaka Suzuki, Youichiro Wada, Hiroyuki Aburatani, Yukiko Kurihara, Sachiko Miyagawa-Tomita, Hiroki Kurihara
Organizer
第19回日本心臓血管発生研究会
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[Presentation] Regionality of intracardiac neural crest cells with diversity and differentiation fate mechanism revealed by single cell and spatial transcriptome analysis2020
Author(s)
Akiyasu Iwase, Yasunobu Uchijima, Daiki Seya, Mayuko Kida, Hiroki Higashiyama, Kazuhiro Matsui, Akashi Taguchi, Shogo Yamamoto, Shiro Fukuda, Seitaro Nomura, Takahide Kohro, Chisa Shukunami, Masahide Seki, Yutaka Suzuki, Youichiro Wada, Hiroyuki Aburatani, Yukiko Kurihara, Sachiko Miyagawa-Tomita, Hiroki Kurihara
Organizer
第43回日本分子生物学会年会
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[Presentation] RUNX/CBF β-driven Irf8 enhancer decides the lineage choice between monocytes and dendritic cells.2020
Author(s)
Koichi Murakami, Haruka Sasaki, Akira Nishiyama, Wataru Kawase, Daisuke Kurotaki, Jun Nakabayashi, Yoichi Sekita, Hideaki Nakajima, Keiko Ozato, Toru Kimura, Tomohiko Tamura
Organizer
第82回日本血液学会学術集会
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[Book] 昆虫と自然2020
Author(s)
稲富桃子, Shin Dungsun, Lai Yi-Ting, 松本竜実, 松野健治
Pages
23-26
Total Pages
36
Publisher
ニュー・サイエンス社
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