2018 Fiscal Year Final Research Report
Analysis of mucosal tolerance by regulatory T cell in chronic otitis media
| Project/Area Number |
16K11187
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
川野 利明 大分大学, 医学部, 助教 (30633424)
鈴木 正志 大分大学, 医学部, 教授 (60211314)
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| Research Collaborator |
SUZUKI MASASHI 大分大学, 医学部, 教授 (60211314)
KAWANO TOSHIAKI 大分大学, 医学部, 助教 (30633424)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | 制御性T細胞 / 粘膜免疫 / 免疫寛容 / B細胞 / 鼻粘膜 / 脾臓 |
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| Outline of Final Research Achievements |
We investigated the effect of regulatory T cell (Treg) on B cell immune responses against outer membrane protein (OMP) from NTHi in vitro. Mice were vaccinated intranasally with OMP , and mononuclear cells (MNCs) were collected from the nasal mucosa, Tregs and helper T cells (Th) were isolated from the spleens of those mice. Three different cell culture groups were allocated: MNCs co-cultured with Tregs, or with Th cells, and MNCs cultured alone, respectively. At 24 and 72 hours after cell culture, cytokine levels were determined by ELISA. CD69 or CD80 expressions on B2 cells were detected by flow cytometryic analysis. IL-10 levels were significantly higher in Treg and Th groups than in the control group. The ratio of CD80+B220+ B2 cells was higher in the control group than in the Treg and Th groups during incubation. Tregs and Th cells may partially inhibit B cell functions, such as T cell activation. These inhibitory effects may be related to IL-10.
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| Free Research Field |
上気道粘膜免疫
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| Academic Significance and Societal Importance of the Research Achievements |
マウス慢性中耳炎由来の制御性T細胞を用いた検討は試料サイズが少ないため、脾臓由来の制御性T細胞やhelper T細胞によるマウス鼻粘膜由来B細胞における影響について検討を行った。現在まで、上気道粘膜由来B細胞におけるT細胞の関与についてin vitroでの解析の報告は少なく、今回は慢性感染と制御性T細胞の関与が上気道においても起こりえる可能性が示唆され、制御性T細胞のB細胞における抗体産生能に与える直接的な抑制効果よりも、特にT-B細胞間の相互作用の抑制効果が上気道粘膜における免疫寛容に関わっていることが示唆された。制御性T細胞は、慢性気道感染における新しい治療標的になるかもしれない。
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