2017 Fiscal Year Final Research Report
Drug discovery of orphan drug for congenital kidney disease using kidney visualization transparent model animal
| Project/Area Number |
16K15468
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Kidney internal medicine
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
秋山 真一 名古屋大学, 医学系研究科, 特任講師 (20500010)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 透明モデル動物 |
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| Outline of Final Research Achievements |
We aimed to develop a novel drug discovery platform for medicine for congenital renal disease which was based on in vivo phenotype screening using kidney-visualized-transparent-zebrafish.
As a result, we bred a new line of transparent zebrafish that is more growth efficiency and more transparency than conventional lines. Although, the gene editing-based congenital nephrotic syndrome model lines could not be generated during study period, the congenital whole-body calcification model transparent kidney visualized line was generated using a phosphorus transporter mutant line. As a result of a compound administration experiment using frying fish, it was possible to observe dose-dependent occurrence of edema, kidney malformation, disappearance of nephron, etc. according to the nephrotoxicity of the compound. These data demonstrated the feasibility of in vivo screening system using kidney visualized transparent zebrafish.
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| Free Research Field |
腎臓内科学
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