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2017 Fiscal Year Final Research Report

In cellulo crystallography with a synchrotron for a human neuraminidase

Research Project

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Project/Area Number 16K18507
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionHigh Energy Accelerator Research Organization

Principal Investigator

Koiwai Kotaro  大学共同利用機関法人高エネルギー加速器研究機構, 物質構造科学研究所, 研究員 (60620721)

Co-Investigator(Renkei-kenkyūsha) ITOH Kohji  徳島大学, 大学院医歯薬学研究部, 教授 (00184656)
Research Collaborator SENDA Toshiya  
YUMOTO Fumiaki  
Project Period (FY) 2016-04-01 – 2018-03-31
Keywordsリソソーム病 / ノイラミニダーゼ / 細胞内結晶 / 放射光 / 構造生物学 / 自由電子レーザー
Outline of Final Research Achievements

The purpose of this research is to clarify a molecular mechanism why mutations causing lysosomal diseases on human neuraminidase-1 (NEU1) reduce its activity. During this period, methods to prepare NEU1 in cellulo crystals have been improved, and X-ray diffraction data were collected from the extracted crystals. Furthermore, high-quality protein solution has been prepared by using the improved method followed by purification. This work opens a way for not only on NEU1 structural analysis but for commonly in cellulo crystallography.

Free Research Field

構造生物学

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Published: 2019-03-29  

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