2017 Fiscal Year Final Research Report
The role of colony-stimulating factors on microglial proliferation and neuropathic pain in rats
| Project/Area Number |
16K19220
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pain science
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 神経障害性疼痛 / 末梢神経 / 損傷 / マイクログリア / 増殖 / コロニー刺激因子 / 脊髄 |
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| Outline of Final Research Achievements |
We examined the expression of mRNAs for macrophage-CSF (M-CSF), granulocyte macrophage-CSF (GM-CSF), granulocyte-CSF (G-CSF) and IL-34 in the dorsal root ganglion (DRG) and spinal cord after peripheral nerve injury in rats. RT-PCR and ISHH revealed that M-CSF and IL-34, but not GM- or G-CSF, mRNAs were constitutively expressed in the DRG, and M-CSF robustly increased in injured-DRG neurons. M-CSF receptor mRNA was expressed in naive rats and increased in spinal microglia following peripehral nerve injury. Intrathecal injection of M-CSF receptor inhibitor partially but significantly reversed the proliferation of spinal microglia and mechanical allodynia induced by peripheral nerve injury. Furthermore, intrathecal injection of recombinant M-CSF induced microglial proliferation and mechanical allodynia.
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| Free Research Field |
神経解剖学
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