Molecular Diagnosis of Breast Cancer Characteristics for Personalized Medicine

2009 Fiscal Year Final Research Report

• Project/Area Number: 17015027
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka University
• Principal Investigator: NOGUCHI Shinzaburo Osaka University, 医学系研究科, 教授 (10303942)
• Co-Investigator(Kenkyū-buntansha): TAMAKI Yasuhiro 大阪大学, 大学院・医学研究科, 准教授 (10273690) ; JIN Kim Seung 大阪大学, 大学院・医学研究科, 准教授 (90346213) ; MIYOSHI Yasuo 兵庫医科大学, 医学部, 准教授 (50283784)
• Project Period (FY): 2005 – 2009
• Keywords: 乳癌 / 予後 / 化学療法感受性 / 遺伝子発現 / 細胞周期 / プロファイリング

Research Abstract

We have been able to construct diagnostic systems for prognosis of breast cancer patients treated with adjuvant hormonal therapy alone, taking advantage of gene expression analysis and cell cycle profiling analysis. Our systems were found to be superior to the conventional clinicopathological prognostic parameters. In addition, we have been able to construct a diagnostic system for response to neoadjuvant chemotherapy consisteing of taxane followed by anthracycline in breast cancer patients.

Research Products

• [Journal Article] Association of breast cancer stem cells identified by aldehyde dehydrogenase 1 expression with resistance to sequential Paclitaxel and epirubicin-based chemotherapy for breast cancers. (2009)
  • Author(s): Tanei, T., Morimoto, K., Shimazu, K., Kim, S.J., Tanji, Y., Taguchi, T., Tamaki, Y., Noguchi, S.
  • Journal Title: Clin Cancer Res 15 Pages: 4234-4241
  • Peer Reviewed
• [Journal Article] Molecular detection of lymph node metastases in breast cancer patients: Results of a multi-centric trial using a fully automated system, the one-step nucleic acid amplification (OSNA) assay. (2009)
  • Author(s): Tamaki, Y., Akiyama, F., Iwase, T., Kaneko, K.,Tsuda, H., Sato, K., Ueda, S., Mano, M., Masuda, N., Takeda, M., Tsujimoto, M., Yoshidome, K., Inaji, H., Nakajima, H., Komoike, Y., Kataoka, T., Nakamura, S., Suzuki, K., Tsugawa, K., Wakasa, K., Okino, T., Kato, Y., Noguchi, S., Matsuura
  • Journal Title: Clin Cancer Res 15 Pages: 2879-2884
  • Peer Reviewed
• [Journal Article] Genetic polymorphisms of CYP2D6*10 and CYP2C19*2, *3 are not associated with prognosis, endometrial thickness, or bone mineral density in Japanese breast cancer patients treated with adjuvant tamoxifen. (2009)
  • Author(s): Okishiro, M., Taguchi, T., Jin Kim, S., Shimazu, K., Tamaki, Y., Noguchi, S.
  • Journal Title: Cancer 115 Pages: 952-961
  • Peer Reviewed
• [Journal Article] Prediction of paclitaxel sensitivity by CDK1 and CDK2 activity in human breast cancer cells. (2009)
  • Author(s): Nakayama, S., Torikoshi, Y., Takahashi, T., Yoshida, T., Sudo, T., Matsushima, T., Kawasaki, Y., Katayama, A., Gohda, K., Hortobagyi, G.N., Noguchi, S., Sakai, T., Ishihara, H., Ueno, N.T.
  • Journal Title: Breast Cancer Res 11 Pages: R12
  • Peer Reviewed
• [Journal Article] Intraoperative frozen section analysis of sentinel lymph node in breast cancer patients treated with neoadjuvant chemotherapy. (2008)
  • Author(s): Shimazu, K., Tamaki, Y., Taguchi, T., Tsukamoto, F., Kasugai, T., Noguchi, S.
  • Journal Title: Ann Surg Oncol 15 Pages: 1717-1722
  • Peer Reviewed
• [Journal Article] Incidence of joint symptoms and bone fractures in Japanese postmenopausal breast cancer patients treated with adjuvant anastrozole. (2008)
  • Author(s): Okishiro, M., Taguchi, T., Kim, S.J., Tanji, Y., Shimazu, K., Tamaki, Y., Noguchi, S.
  • Journal Title: J Cancer Res Clin Oncol 135 Pages: 823-827
  • Peer Reviewed
• [Journal Article] Connexin26 expression is associated with aggressive phenotype in human papillary and follicular thyroid cancers. (2008)
  • Author(s): Naoi, Y., Miyoshi, Y., Taguchi, T., Kim, S.J., Arai, T., Maruyama, N., Tamaki, Y., Noguchi, S.
  • Journal Title: Cancer Lett 262 Pages: 248-256
  • Peer Reviewed
• [Journal Article] Growth-inhibitory effect of adiponectin via adiponectin receptor 1 on human breast cancer cells through inhibition of S-phase entry without inducing apoptosis. (2008)
  • Author(s): Nakayama, S., Miyoshi, Y., Ishihara, H., Noguchi, S.
  • Journal Title: Breast Cancer Res Treat 112 Pages: 405-410
  • Peer Reviewed
• [Journal Article] Low nuclear grade but not cell proliferation predictive of pathological complete response to docetaxel in human breast cancers. (2008)
  • Author(s): Miyoshi, Y., Kurosumi, M., Kurebayashi, J., Matsuura, N., Takahashi, M., Tokunaga, E., Egawa, C., Masuda, N., Kim, S.J., Okishiro, M., Yanagisawa, T., Ueda, S., Taguchi, T., Tamaki, Y., Noguchi, S.
  • Journal Title: J Cancer Res Clin Oncol 134 Pages: 561-567
  • Peer Reviewed
• [Journal Article] Topoisomerase IIalpha-positive and BRCA1-negative phenotype: Association with favorable response to epirubicin-based regimens for human breast cancers. (2008)
  • Author(s): Miyoshi, Y., Kurosumi, M., Kurebayashi, J., Matsuura, N., Takahashi, M., Tokunaga, E., Egawa, C., Masuda, N., Kim, S.J., Okishiro, M., Yanagisawa, T., Ueda, S., Taguchi, T., Tamaki, Y., Noguchi, S.
  • Journal Title: Cancer Lett 26 Pages: 44-53
  • Peer Reviewed
• [Journal Article] Quantitative assessment of mammographic density and breast cancer risk for Japanese women. (2008)
  • Author(s): Kotsuma, Y., Tamaki, Y., Nishimura, T., Tsubai, M., Ueda, S., Shimazu, K., Jin Kim, S., Miyoshi, Y., Tanji, Y., Taguchi, T., Noguchi, S.
  • Journal Title: Breast 17 Pages: 27-35
  • Peer Reviewed
• [Journal Article] Determination of the specific activity of CDK1 and CDK2 as a novel prognostic indicator for early breast cancer. (2008)
  • Author(s): Kim, S.J., Nakayama, S., Miyoshi, Y., Taguchi, T., Tamaki, Y., Matsushima, T., Torikoshi, Y., Tanaka, S., Yoshida, T., Ishihara, H., Noguchi, S.
  • Journal Title: Ann Oncol 19 Pages: 68-72
  • Peer Reviewed
• [Journal Article] Association of GSTP1 expression with resistance to docetaxel and paclitaxel in human breast cancers. (2008)
  • Author(s): Arai, T., Miyoshi, Y., Kim, S.J., Akazawa, K., Maruyama, N., Taguchi, T., Tamaki, Y., Noguchi, S.
  • Journal Title: Eur J Surg Oncol 34 Pages: 734-738
  • Peer Reviewed
• [Journal Article] Potential of reduction in total tumor volume measured with 3D-MRI as a prognostic factor for locally-advanced breast cancer patients treated with primary chemotherapy. (2008)
  • Author(s): Akazawa, K., Tamaki, Y., Taguchi, T., Tanji, Y., Miyoshi, Y., Kim, S.J., Shimazu, K., Ueda, S., Yanagisawa, T., Okishiro, N., Imazato, M., Yasuyuki, K., Sato, Y., Tamura, S., Noguchi, S.
  • Journal Title: Breast J 14 Pages: 523-531
  • Peer Reviewed
• [Journal Article] One-step nucleic acid amplification for intraoperative detection of lymph node metastasis in breast cancer patients. (2007)
  • Author(s): Tsujimoto, M., Nakabayashi, K., Yoshidome, K., Kaneko, T., Iwase, T., Akiyama, F., Kato, Y., Tsuda, H., Ueda, S., Sato, K., Tamaki, Y., Noguchi, S., Kataoka, T. R., Nakajima, H., Komoike, Y., Inaji, H., Tsugawa, K., Suzuki, K., Nakamura, S., Daitoh, M., Otomo, Y., Matsuura, N.
  • Journal Title: Clin Cancer Res 13 Pages: 4807-4816
  • Peer Reviewed
• [Journal Article] Demonstration of Adiponectin Receptors 1 and 2 mRNA expression in human breast cancer cells. (2007)
  • Author(s): Takahata, C., Miyoshi, Y., Irahara, N., Taguchi, T., Tamaki, Y., Noguchi, S.
  • Journal Title: Cancer Lett 250 Pages: 229-236
  • Peer Reviewed
• [Journal Article] Low LATS2 mRNA level can predict favorable response to epirubicin plus cyclophosphamide, but not to docetaxel, in breast cancers. (2007)
  • Author(s): Takahashi, Y., Miyoshi, Y., Morimoto, K., Taguchi, T., Tamaki, Y., Noguchi, S.
  • Journal Title: J Cancer Res Clin Oncol 133 Pages: 501-519
  • Peer Reviewed
• [Journal Article] Possible involvement of CCT5, RGS3, and YKT6 genes up-regulated in p53-mutated tumors in resistance to docetaxel in human breast cancers. (2007)
  • Author(s): Ooe, A., Kato, K., Noguchi, S.
  • Journal Title: Breast Cancer Res Treat 101 Pages: 305-315
  • Peer Reviewed
• [Journal Article] Connexin26 expression is associated with lymphatic vessel invasion and poor prognosis in human breast cancer. (2007)
  • Author(s): Naoi, Y., Miyoshi, Y., Taguchi, T., Kim, S.J., Arai, T., Tamaki, Y., Noguchi, S.
  • Journal Title: Breast Cancer Res Treat 106 Pages: 11-17
  • Peer Reviewed
• [Journal Article] Clinicopathologic Analysis of Breast Cancers with PIK3CA Mutations in Japanese Women. (2007)
  • Author(s): Maruyama, N., Miyoshi, Y., Taguchi, T., Tamaki, Y., Monden, M., Noguchi, S.
  • Journal Title: Clin Cancer Res 13 Pages: 408-414
  • Peer Reviewed
• [Journal Article] Zinc and its transporter ZIP10 are involved in invasive behavior of breast cancer cells. (2007)
  • Author(s): Kagara, N., Tanaka, N., Noguchi, S., Hirano, T.
  • Journal Title: Cancer Sci 98 Pages: 692-697
  • Peer Reviewed
• [Journal Article] A multi-class predictor based on a probabilistic model: application to gene expression profiling-based diagnosis of thyroid tumors. (2006)
  • Author(s): Yukinawa, N., Oba, S., Kato, K., Taniguchi, K., Iwao-Koizumi, K., Tamaki, Y., Noguchi, S., Ishii, S.
  • Journal Title: BMC Genomics 7 Pages: 190
  • Peer Reviewed
• [Journal Article] High expression of ubiquitin carboxy-terminal hydrolase-L1 and -L3 mRNA predicts early recurrence in patients with invasive breast cancer. (2006)
  • Author(s): Miyoshi, Y., Nakayama, S., Torikoshi, Y., Tanaka, S., Ishihara, H., Taguchi, T., Tamaki, Y., Noguchi, S.
  • Journal Title: Cancer Sci 97 Pages: 523-529
  • Peer Reviewed
• [Journal Article] High expression of leptin receptor mRNA in breast cancer tissue predicts poor prognosis for patients with high, but not low, serum leptin levels. (2006)
  • Author(s): Miyoshi, Y., Funahashi, T., Tanaka, S., Taguchi, T., Tamaki, Y., Shimomura, I., Noguchi, S.
  • Journal Title: Int J Cancer 118 Pages: 1414-1419
  • Peer Reviewed
• [Journal Article] Quantitative analysis of aromatase, sulfatase and 17beta-HSD(1) mRNA expression in soft tissue metastases of breast cancer. (2006)
  • Author(s): Irahara, N., Miyoshi, Y., Taguchi, T., Tamaki, Y., Noguchi, S.
  • Journal Title: Cancer Lett 243 Pages: 23-31
  • Peer Reviewed
• [Journal Article] Quantitative analysis of aromatase mRNA expression derived from various promoters (I. 4, I. 3, PII and I. 7) and its association with expression of TNF-alpha, IL-6 and COX-2 mRNAs in human breast cancer. (2006)
  • Author(s): Irahara, N., Miyoshi, Y., Taguchi, T., Tamaki, Y., Noguchi, S.
  • Journal Title: Int J Cancer 118 Pages: 1915-1921
  • Peer Reviewed
• [Journal Article] Association of GSTP1 CpG Islands Hypermethylation with Poor Prognosis in Human Breast Cancers. (2006)
  • Author(s): Arai, T., Miyoshi, Y., Kim, S.J., Taguchi, T., Tamaki, Y., Noguchi, S.
  • Journal Title: Breast Cancer Res Treat 100 Pages: 169-176
  • Peer Reviewed
• [Journal Article] Preoperative evaluation of residual tumor extent by three-dimensional magnetic resonance imaging in breast cancer patients treated with neoadjuvant chemotherapy. (2006)
  • Author(s): Akazawa, K., Tamaki, Y., Taguchi, T., Tanji, Y., Miyoshi, Y., Kim, S.J., Ueda, S., Yanagisawa, T., Sato, Y., Tamura, S., Noguchi, S.
  • Journal Title: Breast J 12 Pages: 130-137
  • Peer Reviewed
• [Journal Article] Differentiation of follicular thyroid adenoma from carcinoma by means of gene expression profiling with adapter-tagged competitive polymerase chain reaction. (2005)
  • Author(s): Taniguchi, K., Takano, T., Miyauchi, A., Koizumi, K., Ito, Y., Takamura, Y., Ishitobi, M., Miyoshi, Y., Taguchi, T., Tamaki, Y., Kato, K., Noguchi, S.
  • Journal Title: Oncology 69 Pages: 428-435
  • Peer Reviewed
• [Journal Article] Down-regulation of LATS1 and LATS2 mRNA expression by promoter hypermethylation and its association with biologically aggressive phenotype in human breast cancers. (2005)
  • Author(s): Takahashi, Y., Miyoshi, Y., Takahata, C., Irahara, N., Taguchi, T., Tamaki, Y., Noguchi, S.
  • Journal Title: Clin Cancer Res 11 Pages: 1380-1385
  • Peer Reviewed
• [Journal Article] Postoperative adjuvant therapy with tamoxifen, tegafur plus uracil, or both in women with node-negative breast cancer: a pooled analysis of six randomized controlled trials. (2005)
  • Author(s): Noguchi, S., Koyama, H., Uchino, J., Abe, R., Miura, S., Sugimachi, K., Akazawa, K., Abe, O.
  • Journal Title: J Clin Oncol 23 Pages: 2172-2184
  • Peer Reviewed
• [Journal Article] Prediction of Response to Docetaxel by Immunohistochemical Analysis of CYP3A4 Expression in Human Breast Cancers. (2005)
  • Author(s): Miyoshi, Y., Taguchi, T., Kim, S.J., Tamaki, Y., Noguchi, S.
  • Journal Title: Breast Cancer 12 Pages: 11-15
  • Peer Reviewed
• [Journal Article] High thioredoxin expression is associated with resistance to docetaxel in primary breast cancer. (2005)
  • Author(s): Kim, S.J., Miyoshi, Y., Taguchi, T., Tamaki, Y., Nakamura, H., Yodoi, J., Kato, K., Noguchi, S.
  • Journal Title: Clin Cancer Res 11 Pages: 8425-8430
  • Peer Reviewed
• [Journal Article] Cancer gene expression database (CGED): a database for gene expression profiling with accompanying clinical information of human cancer tissues. (2005)
  • Author(s): Kato, K., Yamashita, R., Matoba, R., Monden, M., Noguchi, S., Takagi, T., Nakai, K.
  • Journal Title: Nucleic Acids Res 33 Pages: D533-536
  • Peer Reviewed
• [Journal Article] Prediction of docetaxel response in human breast cancer by gene expression profiling. (2005)
  • Author(s): Iwao-Koizumi, K., Matoba, R., Ueno, N., Kim, S.J., Ando, A., Miyoshi, Y., Maeda, E., Noguchi, S., Kato, K.
  • Journal Title: J Clin Oncol 23 Pages: 422-431
  • Peer Reviewed
• [Journal Article] Possible involvement of aromatase overexpression induced by cyclo-oxygenase-2 in the pathogenesis of idiopathic gynecomastia. (2005)
  • Author(s): Irahara, N., Miyoshi, Y., Taguchi, T., Tamaki, Y., Noguchi, S., Harada, N.
  • Journal Title: Endocr Res 31 Pages: 219-227
  • Peer Reviewed
• [Presentation] Breast cancer stem cells identified by ALDH1 expression were associated with resistance to sequential paclitaxel and epirubicin-based chemotherapy for human breast cancers. (2009)
  • Author(s): Tanei, T., Morimoto, K., Shimazu, K., Kim, S.J., Tanji, Y., Taguchi, T., Tamaki, Y., Noguchi, S.
  • Organizer: 32nd San Antonio Breast Cancer Symposium
  • Place of Presentation: San Antonio TX, U.S.A.
  • Year and Date: 2009-12-11
• [Presentation] Ratio of cyclin-dependent kinase 1 (CDK1) activity to CDK2 activity after ex vivo paclitaxel treatment predicts response to paclitaxel in human breast cancer. (2008)
  • Author(s): Kim, S.J., Ishihara, H., Noguchi, S.
  • Organizer: 31st Annual San Antonio Breast Cancer Symposium
  • Place of Presentation: San Antonio TX, U.S.A.
  • Year and Date: 2008-12-12
• [Presentation] Cyclin-dependent kinase 2 to 1 specific activity ratio predicts response to epirubicin and paclitaxel in human breast cancer. (2007)
  • Author(s): Kim, S.J., Ishihara, H., Noguchi, S.
  • Organizer: 30th Annual San Antonio Breast Cancer Symposium
  • Place of Presentation: San Antonio TX, U.S.A.
  • Year and Date: 2007-12-14