2007 Fiscal Year Final Research Report Summary
Molecular mechanism of induction of hemeoxygenase-1 by radiation, hyperthermia , and ultrasound
| Project/Area Number |
17310032
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | University of Toyama |
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Principal Investigator |
KONDO Takashi University of Toyama, Graduate School ofMedicine and Pharmaceutical Sciences, Dept of Radiol Sci, Prof (40143937)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Ryohei University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Dept of Radiol Sci, Assistant, Prof (60334736)
ZHAO Qing-Li University of Toyama, Graduate School of Medicine and Pharmaceutical Sciences, Dept ofRadioL Sci, Res Associate (90313593)
TABUCHI Yoshikai University of Toyama, T ife Science Research Center, Associate Prof (20322109)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Radiation / Hyperthermia / Ultrasound / Microarray / Oxidative stress |
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| Research Abstract |
For better understanding of the molecular mechanism of hemeoxygenase (HO)-1 as a component of cellular response to non-thermal low intensity pulsed ultrasound(LIPUS, 0.3 W/cm2 for 1 min), and mild hyperthermia (MH, 41℃, 30 min), we examined gene expression patterns and genetic networks in human lymphoma U937 cells using high-density oligonucleotide microarrays and computational gene expression analyses. Six hours after LIPUS treatment, apoptosis (14±3.8%) with no cell lysis was observed. Of 22,283 probe sets analyzed, LIPUS down-regulated 193 genes and up-regulated 201 genes by >1.5-fold. On the other hand, 423 genes were up-regulated and 515 genes were down-regulated in the cells 3 h post-treatment with MH which did not induce apoptosis. The significant genetic networks were identified and the interaction between HO-1 and NRG1 or NF2, and BCL2 or CREB1 was observed in the cells treated with LIPUS and MH, respectively. When the cells were exposed to X-rays, up-regulation of HO-1 was observed in 17937 cells but not in HeLa cells.
The present results indicate that LIPUS and MH affect the expression of many genes and provide novel insight into the biomolecular mechanisms of HO-1. The identification of radiation-responsive genes interacting with HO-1 remains a subject for future studies.
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Research Products
(29 results)
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[Journal Article] Molecular mechanism of apoptosis and gene expression in human lymphoma U937 cells treated with anisomycin.2008
Author(s)
Hod, T., Kondo, T., Tabuchi, Y., Takasaki, I., Zhao, Q-L., Kanamori, M., Yasuda, T., Kimura, T
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Journal Title
Chemico-Biol. Int 175
Pages: 125-140
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Idetification of genes responsive to low intensity pulsed ultrasound in a human leukemia cell line Molt-4.2007
Author(s)
Tabuchi, Y., Ando, H., Takasaki, I., Feril, L.B.Jr., Zhao, Q.-L., Ogawa, R., Kudo, N., Tachibana, K., Kondo, T
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Journal Title
Cancer Lett 246
Pages: 149-156
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Identification of gene responsive to paeoniflorin, a heat shock protein-inducing compound, in human leukemia U937 cells.2007
Author(s)
Salunga, T.L., Tabuchi, Y., Takasaki, I., Fed, L.B., Jr., Zhao, QL., Ohtsuka, K., Tsuneyama, K., Kondo, T
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Journal Title
Int. J. Hyperthermia 23
Pages: 529-537
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Enhancement of hyperthermia-induced apoptosis by a new synthesized class of furan-fused tetracyclic compounds.2007
Author(s)
Yu, D.-Y., Matsuya, Y., Zhao, Q.-L., Ahemd, K., Wei, Z.-L., Nemoto, H., Kondo, T
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Journal Title
Apoptosis 12
Pages: 1523-1532
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Confirmation of enhanced expression of heme oxygenase-1 gene induced by ultrasound and its mechanism: Analysis by cDNA microarray system, real time quantitiative PCR, and western blotting.2006
Author(s)
Kagiya, G, Tabuchi, Y, Feril LB, Jr, Ogawa, R, Zhao, Q-L, Kudo, N, Hiraoka, W, Tachibana, K, Umemura, S-I, Kondo, T
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Journal Title
J. Med. Ultrasonics 33
Pages: 3-10
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic networks responsive to sodium butyrate in colonic epithelial cells.2006
Author(s)
Tabuchi, Y, Takasaki, I, Doi, T, Ishii, Y, Sakai, H, Kondo, T
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Journal Title
FEBS Lett 580
Pages: 3035-3041
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Expression of heme oxygenase-1 due to intracellular reactive oxygen species induced by ultrasound.2006
Author(s)
Kagiya, G, Ogawa, R, Tabuchi, Y, Feril LB, Jr, Nozaki, T, Fukuda, S, Yamamoto, K, Kondo, T
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Journal Title
Ultrason. Sonochem 13
Pages: 388-396
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Analysis of gene expression in apoptosis of human lymphoma U937 cells induced by heat shock and the effects of 6-phenyl N-tert-butylnitrone(PBN)and its derivatives.2005
Author(s)
Hirano, H., Tabuchi, Y., Kondo, T., Zhao, Q-L., Ogawa, R., Cui, Z-G., Feril, Jr. L. B., Kanayama, S
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Journal Title
Apoptosis 10
Pages: 331-340
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Apoptosis induced by the sonomechanical effects of low intensity pulsed ultrasound2005
Author(s)
Feril, Jr., L. B., Kondo, T., Cui, Z-G., Tabuchi, Y., Zhao, Q-L., Ando, H., Misaki, T., Yoshikawa, H., Umemura, S
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Journal Title
Cancer Lett 221
Pages: 145-152
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Bioeffect of ultrasound. Apoptosis and change of gene expression induced by ultrasound and their therapeutic utilization.2005
Author(s)
Kondo, T., Feril Jr., L. B., Ogawa, R., Zhao, Q-L., Honda, H., Nozaki, T., Kagiya, G., Cui, Z-G., Ando, H., Tabuchi Y
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Journal Title
In 4th International Symposium Therapeutic Ultrasound,(by ter Harr G. R., and Rivens I.(Ed.)) 261-263(American Institute of Physics AIP conference Proceedings 754 New York) 754
Pages: 261-263
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic networks responsive to low intensity pulsed ultrasound in human lymphoma U937 cells
Author(s)
Tabuchi, Y., Takasakia, I., Zhao Q.-L., Wada, S., Hod, T., Feril, L.B.Jr., Nomura, T., Kondo, T
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Genetic networks responsive to mild hyperthermia in human lymphoma U937 cells.
Author(s)
Tabuchi, Y., Takasakia, I., Zhao, Q.-L., Wada, S., Hon, T., Feril, Nomura, T., Kondo, T
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Journal Title
Int. J. Hyperthermia (in press)
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Biomolecular effects of low intensity ultrasound: apoptosis and gene expression.2007
Author(s)
Kondo, T, Ogawa, R, Zhao, QL, Tabuchi, Y, Kudo, N, Feril, LB, Jr
Organizer
International Congress of Ultrasonics
Place of Presentation
Vienna, Autria
Year and Date
20070409-12
Description
「研究成果報告書概要(欧文)」より
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