2007 Fiscal Year Final Research Report Summary
Dynamics of protein structure alteration at single molecular level
| Project/Area Number |
17360395
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Japan Advanced Institute of Science and Technology |
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Principal Investigator |
TAKAGI Masahiro Japan Advanced Institute of Science and Technology, School of Materials Science, Professor (00183434)
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| Co-Investigator(Kenkyū-buntansha) |
HOSAKA Takahiro Japan Advanced Institute of Science and Technology, School of Materials Sciences, Associate Professor (30263619)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Protein / Structure / Amyloid / Membrane / Interaction |
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| Research Abstract |
(1) In order to perform analysis of protein structural alteration at single molecular level using FRET method We attempted fluorescence labeling of bavine pancreatic tripsin inhibitor (BFTI) using a new technique of four base codon method. Fluorescent proteins were obtained but not enough yield for studies at molecular level
(2) The effects of negatively charged phosphatidylserine (PS) and neutral phosphatidylcholine (PC) on the structure of wild-type and mutant bovine pancreatic trypsin inhibitor (BPTI) at neutral pH were investigated. The presence of PC did not have any effect on the protein structure whilst PS induced a non-native structure in three mutant BPTI proteins. However, the negatively charged phospholipid did not have any effect on wild-type BPTI. The findings revealed that (i) elimination of some disulphide bonds, results in dramatic change in protein structure, and, (ii) that this biochemical interaction is surface-driven and that electrostatic interactions may play a ver
… More
y strong role in influencing the fore-stated changes in protein structure.
(3) Patients on long term hemodyalysis frequently suffer from dialysis-related amyloidosis (DRA). DRA is a pathology associated with a persistent increase of monomeric β2- microglobulin (β2-m) caused by dialysis treatment of renal failure. The mechanism of formation of amyloid fibrils for this protein, as for all the other amyloidogenic proteins, is not clear, and there was great uncertainty about the molecular bases of amyloid deposition when the amyloidogenicity of β2-m was discovered. In this study, we investigated formation of variant β2-m in serum of a patient who receives treatment of hemodialysis. Variant β2-m is derived from restricted cleavage of β2-M. The possible factor involved in formation of variant β2-m was also estimated. The following conclusions are proposed. Formation of variant β2-m, namely restricted cleavage of β2-m, might be caused by proteolytic cleavage by a serum protease(s). However, it is not clear whether the variant β2-m can trigger formation of amyloid. Protein aggregation formation is enhanced in serum from a patient. Less
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Research Products
(94 results)
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[Journal Article] Effect of Phospholipids on conformational structure of bovine pancreatic trypsin inhibitor (BPTI) and its thermolabile mutants [Epub ahead of print]2008
Author(s)
Izumikawa, N., Nishikori, S., Vestergaard, M., Hamada, T., Hagihwara, Y., Yumoto, N., Shiraki, K., Takagi, M
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Chelation of Cadmium Ions by Phytochelatin Synthase : Role of the Cystein-rich C-Terminal, Abstract2008
Author(s)
Vestergaard, M., Matsumoto, S., Nishikori, S., Shiraki, K., Hirata, K., Takagi, M
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Journal Title
Anal Sci 24
Pages: 277-281
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Dynamic Processes in Endocytic Transformation of a Raft-Exhibiting Giant Liposome2007
Author(s)
Hamada, T., Miura, Y., Ishii, K., Araki, S., Yoshikawa, K., Vestergaard, M., Takagi, M
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Journal Title
J. Phys. Chem. B 111
Pages: 10853-10857
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Enhancing the Tolerance of Zebrafish (Danio rerio) to Heavy Metal Toxicity by the Expression of Plant Phytochelatin Synthase2006
Author(s)
Konishi, T., Matsumoto, S., Tsuruwaka, Y., Shiraki, K., Hirata, K., Tamaru, Y., Takagi, M
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Journal Title
J. Biotechnol 122
Pages: 316-325
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Crystal Structure of Intein Homing Endonuclease II Encoded in DNA Polymerase Gene from Hyperthermophilic Archaeon Thermococcus kodakaraensis Strain KOD1 Proteins : Structure2006
Author(s)
Matsumura, H., Takahashi, H., Inoue, T., Yamamoto, T., Hashimoto, H., Nishioka, M., Fujiwara, S., Takagi, M., Imanaka, T., Kai, Y
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Journal Title
Function and Bioinformatics 63
Pages: 711-715
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The First Thermodynamic Characterization of □-1,3-Xylanase from a Marine Bacterium2005
Author(s)
Okazaki, F., Shiraki, K., Tamaru, Y., Araki, T., Takagi, M
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Journal Title
Protein J 24
Pages: 413-421
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Molecular Cloning and Characterization of Alpha-class Glutathione S-Transferase Genes from the Hepatopancreas of Red sea Bream, Pagrus major2005
Author(s)
Konishi, T., Kato, K., Araki, T., Shiraki, K., Takagi, M., Tamaru, Y
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Journal Title
Comp Biochem Physiol. C Toxicol. Pharmacol 140
Pages: 309-320
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Comparative Analysis of the Two-step Reaction Catalyzed by Prokaryotic and Eukaryotic Phytochelatin Synthase by an Ion-pair Liquid Chromatography Assay2005
Author(s)
Tsuji, N., Nishikori, S., Matsumoto, S., Iwabe, O., Shiraki, K., Miyasaka, H., Takagi, M., Hirata, K., Miyamoto, K
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Journal Title
Planta 222
Pages: 181-191
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A New Class of Glutathione S-Transferase from the Hepatopancreas of the Red Sea Bream, Pagrus major.2005
Author(s)
Konishi, T., Kato, K., Araki, T., Shiraki, K., Takagi, M., Tamaru, Y
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Journal Title
Biochem. J 388
Pages: 299-307
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 細胞膜ラフトドメインの動的構造と安定性2007
Author(s)
鈴木えり子, 山口健太郎, 浜田 勉, 高木昌宏
Organizer
日本化学会近畿支部北陸地区研究発表会
Place of Presentation
金沢大学(角間キャンパス)自然科学本館
Year and Date
2007-11-10
Description
「研究成果報告書概要(和文)」より
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[Presentation] 脂質二分子膜形態の光制御2007
Author(s)
石井健一, 長崎 健, 浜田 勉, 高木昌宏
Organizer
日本生物工学会
Place of Presentation
広島大学
Year and Date
2007-09-25
Description
「研究成果報告書概要(和文)」より
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