2019 Fiscal Year Annual Research Report

Development of a new high-throughput TCR-antigen pair identification method toward cancer immune therapy

Research Project

Project/Area Number	17F17389
Research Institution	Institute of Physical and Chemical Research
Principal Investigator	城口克之国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (00454059)
Co-Investigator(Kenkyū-buntansha)	JIN JIANSHI 国立研究開発法人理化学研究所, 生命機能科学研究センター, 外国人特別研究員
Project Period (FY)	2017-10-13 – 2020-03-31
Keywords	イメージング / ピッキング / 細胞相互作用 / Ｔ細胞
Outline of Annual Research Achievements	We have been building up a microscopic platform for scanning the real-time cell behaviors, such as cell movement and T cell-target cell interactions. For high-throughput scanning of these behaviors, we have checked that scanning rate of the current system, and found that the speed of stage movement may be enough for the observation of T cell activities. However, in order to link the microscope system to following steps, we found that real-time calculation based on obtained cell images was required. Then, we investigated specifications of a software based on our actual measurements for T cell-target cell interactions. This helps one to combine imaging and following genomic analyses using our cell picking system.
Research Progress Status	令和元年度が最終年度であるため、記入しない。
Strategy for Future Research Activity	令和元年度が最終年度であるため、記入しない。