Development of extracellular matrix mimicking method for the regulation of stem cell functions by constructive approach

2019 Fiscal Year Final Research Report

• Project/Area Number: 17H04741
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo Metropolitan Industrial Technology Research Institute (2018-2019); Yamagata University (2017)
• Principal Investigator: Hoshiba Takashi 地方独立行政法人東京都立産業技術研究センター, 開発本部開発第二部バイオ応用技術グループ, 研究員 (00469769)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Keywords: 細胞外マトリックス / 培養基板 / 組織工学 / 幹細胞 / 分化 / がん / 脱細胞化

Outline of Final Research Achievements

Regulation of cell function is important in tissue engineering. Particularly, many cell culture substrates, scaffold of cultured cells, are actively developed for this purposes. The scaffold in vivo is extracellular matrix (ECM) and ECM is composed of various components. Therefore, it is difficult to mimic ECM by conventional chemical methods. Generally, the substrates mimicking ECM is prepared by decellularization. To prepare the substrates more easily than decellularization, the constructive approach was tried to applied. the mechanisms of functions of cancer cells and myoblasts were investigated on the decellularized ECM substrates to explore ECM functional modules. And then, the modules were immobilized on the substrates to regulate cell functions, particularly chemoresistance on cancer cells.

Free Research Field

生体材料

Academic Significance and Societal Importance of the Research Achievements

培養基板は産業界で多く開発されてきているが、特に組織工学・再生医療の伸展により、細胞機能を制御できるものが求めれつつある。そのために、様々な生理活性物質を修飾する手法がとられているが、目的の機能をえるには試行錯誤やスクリーニングが必要である。本研究の成果は、上記のような培養基板を設計するための新しい手法となる可能性があるとともに、学術的にも細胞外マトリックスの役割を調べるための一つの手法となる可能性がある。