Development of dental pulp regeneration therapy applying human dental pulp stem cells and fish collagen that has no potential for infectious diseases

2020 Fiscal Year Final Research Report

• Project/Area Number: 17K11806
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Nagasaki University
• Principal Investigator: YAMAMOTO Kohei 長崎大学, 病院(歯学系), 医員 (20756407)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Keywords: 魚由来コラーゲン / 歯髄幹細胞

Outline of Final Research Achievements

We have been studying the application of fish type I atelo collagen (FAC) as a scaffolding material, which has no possibility of zoonotic diseases. In this study, the safety and efficacy of fish-derived collagen as a scaffolding material were evaluated as a non-clinical study using canine teeth in combination with the FAC safety study.
The virus denial test was negative. The ISO standard tests for endotoxin level, cytotoxicity, sensitization, chromosomal abnormalities, intradermal irritation, acute systemic toxicity, febrile sensitivities, and hemolytic anemia were all within the standard values. Our findings on canine pulp regeneration using clinical grade FAC showed no rejection of the new pulp tissue.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

人獣共通感染症への可能性のない魚由来タイプⅠコラーゲン（fish typeⅠatelo collagen:FAC ）を足場材として応用するため検討を重ねてきた。臨床等級のFACを使用したイヌの歯髄再生に関する本所見では、新生した歯髄組織に拒絶反応は認めなかった。
これはFACの大動物への応用の安全性と有効性を証明した最初の報告であり足場材として哺乳類由来のコラーゲンの代替材料として有用であることを示唆した。