2019 Fiscal Year Final Research Report
A study using a high-resolution time-of-flight mass spectrometer to elucidate the cause of sudden death during post-exercise control
| Project/Area Number |
17K19782
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Society medicine, Nursing, and related fields
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
橋谷田 真樹 関西医科大学, 医学部, 准教授 (40374938)
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| Project Period (FY) |
2017-06-30 – 2020-03-31
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| Keywords | 興奮性譫妄 / 突然死 / ストレス / 飛行時間型質量分析 / 主成分分析 / 制圧 / 飲酒 |
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| Outline of Final Research Achievements |
The purpose of this study is to search for biomarkers that can be diagnostic criteria for "excited delirium". We attempted comprehensive metabolomics analysis of rat samples using a high speed liquid chromatogram - tandem mass spectrometer.
As a result, more than 2000 kinds of components were detected, and 119 components (positive) and 149 components (negative) remained.When compounds were identified using a public database and subjected to multivariate analysis, xanthosine appeared to be the most suitable marker candidate.Differences in the increase and decrease of metabolites were observed with and without alcohol intake, and it was confirmed that in metabolomics, slight changes in the body had a great influence on the types and expression of metabolites.
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| Free Research Field |
法医学
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| Academic Significance and Societal Importance of the Research Achievements |
今回の研究の最も大きな成果はG-Met分析を用いることによって，死直前のストレスの違いを識別できた点である．これはもちろんラットを用いた動物実験でのことではあるが，これまで同様なモデルで検討されてきたmiRNA等のマーカーよりも非常に鮮明に違いを表していた．しかしながら，法医試料への応用には今回は検討していない死後変化の影響を加味する必要がある．さらに，運動・抑制以外のストレスや疾患の影響等も考慮しなければならないであろう．現段階では，剖検試料からの判断は難しいと思われるが，さらにデータを重ね，バイオマーカーとしての精度を高めることができればヒトへの応用が十分可能であると思われる.
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