The creation of the de novo vector for the newly Cell Delivery System (CDS)

2021 Fiscal Year Final Research Report

• Project/Area Number: 17K20103
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering and related fields
• Research Institution: Kyoto University
• Principal Investigator: Mitsunaga Kanae 京都大学, iPS細胞研究所, 特定拠点助教 (10398240)
• Project Period (FY): 2017-06-30 – 2022-03-31
• Keywords: CDS / セルデリバリーシステム / HM,BTK / sashimi / ImageStream / Myxsozoa / 共存培養技術

Outline of Final Research Achievements

In this study, I aimed to cure intractable systemic diseases such as ALS, and developed a culture method for efficient cell body acquisition in order to create a carrier (vector) that carries therapeutic cells throughout the body.
Succeeded in developing the cell body separation technology "Free-Amine Isolation Method" using the host materials HM: flatfish and tuna ,BTK: bonito, sea bream, and bonito as the main materials.Confirmation and treatment of cell bodies by ImageStream. Mixozoa was co-cultured by the presence or absence of culture for cell development, slice culture method, etc. As a result, the survival rate at the time of recovery of the collected Myxozoa was slightly improved, and a stable culture method for Myxozoa to be developed as a vector.Although the purpose of this study could not be achieved, the initial research concept is a 30-year plan, and it is easy to acquire “Sashimi” to be used as a material, so I will continue to develop cell delivery systems (CDS).

Free Research Field

生物工学、発生生物学

Academic Significance and Societal Importance of the Research Achievements

本研究では、筋ジストロフィー症などの全身性難病の治癒を目標とし、治療細胞を全身に運ぶ運び屋（ベクター）を作成するため、細胞体の分取と培養方法の開発を行なった。治療細胞を本研究で開発する細胞体に寄与させ、全身に治療細胞を運ぶ技術である。宿主材料には、日頃食するさしみ、HM:ヒラメ、マグロ,BTK:鰤、鯛、鰹を利用した。さしみ組織をターゲットとして寄生する細胞体の性質を利用した。
成果として、細胞体分離技術「フリーアミン寄生生物分離法」開発の成功に至り、少なくとも寄生生物の培養が可能であることがわかった。本成果は移植医療の発展とミクソゾア類生態の解明及び養殖業での病害虫被害への応用にも期待できる