Elucidation of the molecular mechanism to maintain hierarchical structure of vascular system and and tumour angiogenesis

2020 Fiscal Year Final Research Report

• Project/Area Number: 18H02363
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 42040:Laboratory animal science-related
• Research Institution: Shiga University of Medical Science
• Principal Investigator: Ema Masatsugu 滋賀医科大学, 動物生命科学研究センター, 教授 (60359578)
• Co-Investigator(Kenkyū-buntansha): 水野 聖哉 筑波大学, 医学医療系, 准教授 (10633141) ; 杉山 文博 筑波大学, 医学医療系, 教授 (90226481)
• Project Period (FY): 2018-04-01 – 2021-03-31
• Keywords: 血管新生 / 血管内皮細胞 / マウス / 遺伝子改変

Outline of Final Research Achievements

We have identified Exoc3-like family genes which are specifically expressed in developing endothelial cells. To investigate the physiological roles of Exoc3L genes, we have created Exoc3L2-GFP knock-in mouse and found that GFP (Exoc3L2) is expressed in endothelial cells and KO embryos die in utero due to breeding. We also created Exoc3L4-GFP knock-in mouse and found GFP (Exoc3L4) is expressed in endothelial cells as well as cardiomyocytes. Exoc3L4 KO embryos die around E12.5, due to unknown mechanism.
We also created VEGFR3-Venus BAC Tg mouse useful for visualization of vascular and lymphatic endothelial cells.

Free Research Field

発生工学

Academic Significance and Societal Importance of the Research Achievements

Exoc3L遺伝子群の生理機能を体系的に明らかにし、その制御機構を解明することができれば、新たな腫瘍血管新生抑制療法の開発に繋がるものと期待される。