Glycotechnological approaches for prevention of mother-to-child malarial transmission

2009 Fiscal Year Final Research Report

• Project/Area Number: 19570119
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Hirosaki University
• Principal Investigator: KAKIZAKI Ikuko Hirosaki University, 大学院・医学研究科, 准教授 (80302024)
• Project Period (FY): 2007 – 2009
• Keywords: プロテオグリカン / グリコサミノグリカン / コンドロイチン硫酸 / 糖鎖加水分解酵素 / ヒアルロニダーゼ / 糖転移反応 / オリゴ糖

Research Abstract

We developed a new technology to custom synthesize chondroitin sulfate oligosaccharides using re-usable immobilized glycosidase with high purity and high yield reaction products. The synthetic oligosaccharides with defined structures were then used in experiments, gaining structural information that may be useful in the prevention of mother-to-child malarial transmission.

Research Products

• [Journal Article] Mechanism for the hydrolysis of hyaluronan oligosaccharides by bovine testicular hyaluronidase (2010)
  • Author(s): Kakizaki, I., Ibori, N., Kojima, K., Yamaguchi, M., Endo M.
  • Journal Title: FEBS J. 277(7) Pages: 1776-1786
• [Journal Article] 医学応用を目指したプロテオグリカンの糖鎖工学 (特集/糖質科学の機能探求) (2010)
  • Author(s): 柿崎育子
  • Journal Title: 月刊「化学工業」 Vol. 61, No. 3 Pages: 216-220
• [Journal Article] Novel products in hyaluronan digested by bovine testicular hyaluronidase (2009)
  • Author(s): Chen, F., Kakizaki, I., Yamaguchi, M., Kojima, K., Takagaki, K., Endo, M.
  • Journal Title: Glycoconj. J. 26(5) Pages: 559-566
• [Journal Article] Structural Interactions in Chondroitin 4-Sulfate-mediated Adherence of Plasmodium falciparum-infected Erythrocytes in Human Placenta During Pregnancy-Associated Malaria (2008)
  • Author(s): Achur, R. N., Kakizaki, I., Goel, S., Kojima, K., Madhunapantula, S. V., Ohta, M., Kumar, S., Takagaki, K., Gowda, D. C.
  • Journal Title: Biochemistry 47(47) Pages: 12635-12643
• [Journal Article] Up-regulation of hyaluronan synthase genes in cultured human epidermal keratinocytes by UVB irradiation (2008)
  • Author(s): Kakizaki, I., Itano, N., Kimata, K., Hanada, K., Kon, A., Yamaguchi, M., Takahashi, T., Takagaki, K.
  • Journal Title: Arch. Biochem. Biophys. 471(1) Pages: 85-93
• [Journal Article] Diversity in the degree of sulfation and chain length of the glycosaminoglycan moiety of urinary trypsin inhibitor isomers (2007)
  • Author(s): Kakizaki, I., Takahashi, R., Ibori, N., Kojima, K., Takahashi, T., Yamaguchi, M., Kon, A., Takagaki, K.
  • Journal Title: Biochim. Biophys. Acta. 1770(2) Pages: 171-177
• [Presentation] 大会特別シンポジウム【東北から発信する糖質関連新技術】,「医学応用を目指したプロテオグリカンの糖鎖工学」 (2009)
  • Author(s): 柿崎育子
  • Organizer: 日本応用糖質科学会平成21年度大会 (第58回)
  • Place of Presentation: 弘前市
  • Year and Date: 2009-09-17
• [Presentation] ヒアルロニダーゼ固定化カラムを用いた糖転移反応の再検討 (2007)
  • Author(s): 柿崎育子, 須藤晋一郎, 今淳, 山口真範, 遠藤正彦
  • Organizer: 第30回日本分子生物学会年会 第80回日本生化学会大会 合同年会
  • Place of Presentation: 横浜
  • Year and Date: 2007-12-14
• [Book] Endoglycosidases (Glycosaminoglycans) in Experimental Glycoscience-Glycochemistry (edited by Taniguchi, N., Suzuki, A., Ito, Y., Narimatsu, H., Kawasaki, T., Hase, S) (2008)
  • Author(s): Kakizaki, I., Takagaki, K.
  • Total Pages: 173-176
  • Publisher: Springer
• [Book] Degradation of glycosaminoglycans, in Comprehensive Glycoscience-from Chemistry to Systems Biology (edited by J. P. Kamerling., G. J. Boons., Y. C. Lee., A. Suzuki., N. Taniguchi., A. G. J. Voragen., Elsevier Science B. V.) (2007)
  • Author(s): Takagaki, K., Kakizaki, I.
  • Total Pages: 171-192
  • Publisher: Elsevier Science B. V.
• [Remarks] ホームページ等
  • URL: http://www.med.hirosaki-u.ac.jp/~bioche1/
• [Patent(Industrial Property Rights)] ヒアルロニダーゼ阻害剤 (2009)
  • Inventor(s): 遠藤正彦, 柿崎育子, 小泉英誉
  • Industrial Property Rights Holder: 弘前大学
  • Industrial Property Number: 特許特願2009-221568
  • Filing Date: 2009-09-25
• [Patent(Industrial Property Rights)] 糖鎖改変方法 (2008)
  • Inventor(s): 柿崎育子, 遠藤正彦
  • Industrial Property Rights Holder: 弘前大学
  • Industrial Property Number: 特許特願2008-133815(特開2009-278907)
  • Filing Date: 2008-05-22
• [Patent(Industrial Property Rights)] 糖鎖改変ウリナスタチンの製造方法 (2007)
  • Inventor(s): 柿崎育子, 高垣啓一, 高橋良樹
  • Industrial Property Rights Holder: 弘前大学
  • Industrial Property Number: 特許特願2007-90839(特開2008-247802)
  • Filing Date: 2007-03-30