2010 Fiscal Year Final Research Report
Oxygen carrying mechanism of hemoglobin : verification of trigger model by using proximal cavity mutant hemoglobin
| Project/Area Number |
19579002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Kanazawa University |
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Principal Investigator |
AKI Yayoi Kanazawa University, 保健学系, 助教 (00452110)
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| Project Period (FY) |
2007 – 2010
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| Keywords | ヘモグロビン / 酸素運搬機能 / 高次構造変化 / 引き金説 |
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| Research Abstract |
Many people believe that the trigger of the quaternary structural transition of hemoglobin (Hb) is the displacement of the proximal His, which is the only amino acid binding directly to the heme iron (trigger model). I inspected the model by synthesizing the recombinant Hbs, in which the proximal His is replaced with Gly in the α or β subunit, and then analyzing the conformational change and the oxygen carrying mechanism of these Hbs. I have suggested that the shift of the proximal His in the α subunit is the trigger of the structural transition of Hb, whereas one of the β subunit is not the trigger because the conformational change is partially observed in the recombinant Hb without the β proximal His.
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[Journal Article] Effect of reversed heme orientation on circular dichoroism and cooperative oxygen binding of human adult hemoglobin2008
Author(s)
Nagai, M., Nagai, Y., Aki, Y., Imai, K., Wada, Y., Nagatomo, S., Yamamoto, Y.
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Journal Title
Biochemistry 47
Pages: 517-525
Peer Reviewed
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[Journal Article] Oxygenation properties of extracellular giant hemoglobin from Oligobrachia mashikoi2007
Author(s)
Aki, Y., Nakagawa, T., Nagai, M., Sasayama, Y., Fukumori, Y., Imai, K.
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Journal Title
Biochemical and Biophysical Research Communications 360
Pages: 673-678
Peer Reviewed
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