Elucidation of erythrocyte recognition and invasion by Babesia parasites

2009 Fiscal Year Final Research Report

• Project/Area Number: 19580334
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: YOKOYAMA Naoaki Obihiro University of Agriculture and Veterinary Medicine, 原虫病研究センター, 准教授 (80301802)
• Co-Investigator(Kenkyū-buntansha): IGARASHI Ikuo 国立大学法人帯広畜産大学, 原虫病研究センター, 教授 (80159582)
• Project Period (FY): 2007 – 2009
• Keywords: 寄生体生物

Research Abstract

Sialic acids and Heparan sulfate located on erythrocytes were demonstrated to play important roles as infectious receptors in the erythrocyte invasion by Babesia parasites. In contrast, several protozoan proteins were also identified as new protozoan ligand candidates. Effects of glycoconjugates or specific antibodies on interfering the specific interactions between the protozoan ligands and erythrocyte receptors would provide us novel knowledge or materials for the development of chemotherapeutic and preventive mesures against babesionsis.

Research Products

• [Journal Article] Evaluation of the in vitro growth-inhibitory effect of epoxomicin on Babesia parasites (2010)
  • Author(s): AbouLaila. M., Nakamura, K., Govind, Y., Yokoyama, N., Igarashi, I.
  • Journal Title: Vet. Parasitol. 167 Pages: 19-27
  • Peer Reviewed
• [Journal Article] Development and evaluation of a nested PCR based on spherical body protein 2 gene for the diagnosis of Babesia bovis (2010)
  • Author(s): AbouLaila. M., Yokoyama, N., Igarashi, I.
  • Journal Title: Vet. Parasitol. 169 Pages: 45-50
  • Peer Reviewed
• [Journal Article] Inhibitory effect of terpene nerolidol on the growth of Babesia parasites (2010)
  • Author(s): AbouLaila, M., Sivakumar, T, Yokoyama, N., Igarashi, I.
  • Journal Title: Parasitology International 59 Pages: 278-82
  • Peer Reviewed
• [Journal Article] Isolation and pathogenic characterization of an OB1 variant of Babesia rodhaini which has a glycophorin A-independent pathway to murine red blood cells (2009)
  • Author(s): Takabatake, N., Iseki, H., Ikehara, Y., Kanuka, H., Yokoyama, N., Sekimizu, K., Igarashi, I.
  • Journal Title: Vet. Parasitol. 159 Pages: 97-104
  • Peer Reviewed
• [Journal Article] Evaluation of Babesia bigemina 200 kDa recombinant antigen in enzyme-linked immunosorbent assay (2009)
  • Author(s): Altangerel, K, Alhassan, A., Iseki, H., Sivakumar, T., Damdinsuren, B., Yokoyama, N., Igarashi, I
  • Journal Title: Parasitol. Res. 105 Pages: 249-54
  • Peer Reviewed
• [Journal Article] Cloning and Characterization of Peroxiredoxin in Babesia bovis (2009)
  • Author(s): Tanaka, M., Sakurai, T., Yokoyama, N., Inoue, N., Kawazu, S.
  • Journal Title: Parasitol. Res. 105 Pages: 1473-1477
  • Peer Reviewed
• [Journal Article] Molecular and immunological characterization of Babesia gibsoni and Babesia microti heat shock protein-70 (2009)
  • Author(s): Terkawi, M. A., Aboge, G., Jia, H., Goo, Y. K., Ooka, H., Yamagishi, J., Nishikawa, Y., Yokoyama, N., Igarashi, I., Kawazu, S-.I., Fujisaki, K., Xuan, X.
  • Journal Title: Parasite Immunol. 31 Pages: 328-340
  • Peer Reviewed
• [Journal Article] Inhibitory effect of (-)-Epigallocatechin-3-gallate from green tea on the growth of Babesia parasites (2009)
  • Author(s): AbouLaila, M. Yokoyama, N., Igarashi I.
  • Journal Title: Parasitology 22 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Expression analysis of a Babesia bovis BboP67 gene homologous to the Theileria parva p67 gene (2009)
  • Author(s): Sivakumar, T., Khukhuu, A., Alhassan, A., Aboulaila, M. R. A., Iseki, H., Ota, N., Lijia, Z., Yokoyama, N., Igarashi, I.
  • Journal Title: J. Protozool. Res. 19 Pages: 62-71
  • Peer Reviewed
• [Journal Article] Inhibitory effect of apicidin on in vitro and in vivo growth of Babesia parasites (2009)
  • Author(s): Munkhjargal, T., Aboulaila, M. R. A., Sivakumar, T., Yokoyama, N., Igarashi, I.
  • Journal Title: J. Protozool. Res. 19 Pages: 41-49
  • Peer Reviewed
• [Journal Article] Effect of oligomannose-coated liposome-based vaccine on rodent babesiosis (2009)
  • Author(s): Ishigame, T., Takabatake, N., Iseki, H., Ota, N., Koyama, A., Igarashi, I., Nishikawa, Y., Ikehara, Y., Kojima, N., Yokoyama, N.
  • Journal Title: J. Protozool. Res. 19 Pages: 10-23
  • Peer Reviewed
• [Journal Article] The Babesia bosis BvP36 gene is expressed at least in the asexual stage of Babesia merozoite (2009)
  • Author(s): Khukhuu, A., Sivakumar, T., Alhassan, A., Ota, N., Yokoyama, N., Igarashi, I.
  • Journal Title: J. Protozool. Res. 19 Pages: 34-41
  • Peer Reviewed
• [Journal Article] Diagnostic real-time PCR assay for the quantitative detection of Theileria equi from equine blood samples (2008)
  • Author(s): Kim, C., Blanco, L. B. C., Alhassan, A., Iseki, H., Yokoyama, N., Xuan, X., Igarashi, I.
  • Journal Title: Vet. Parasitol. 151 Pages: 158-163
  • Peer Reviewed
• [Journal Article] Development of a rapid immunochromatographic test for simultaneous serodiagnosis of bovine Babesioses caused by Babesia bovis and Babesia bigemina (2008)
  • Author(s): Kim, C., Blanco, L. B. C., Alhassan, A., Iseki, H., Yokoyama, N., Xuan, X., Igarashi, I.
  • Journal Title: Am. J. Trop. Med. Hyg. 78 Pages: 117-121
  • Peer Reviewed
• [Journal Article] Babesia : the protective effects of killed Propionibacterium acnes on the infections of two rodent Babesia parasites in mice (2008)
  • Author(s): Iseki, H., Takabatake, N., Ota, N., Ishigame, T., Yokoyama, N., Igarashi, I.
  • Journal Title: Exp. Parasitol. 118 Pages: 543-548
  • Peer Reviewed
• [Journal Article] C3 contributes to the cross-protective immunity induced by Babesia gibsoni phosphoriboprotein P0 against a lethal B. rodhaini infection (2008)
  • Author(s): Terkawi, M. A., Zhang, G., Jia, H., Aboge, G., Goo, Y. K., Nishikawa, Y., Yokoyama, N., Igarashi, I., Kawazu, S. I., Fujisaki, K., Xuan, X.
  • Journal Title: Parasite Immun. 30 Pages: 365-370
  • Peer Reviewed
• [Journal Article] In vitro inhibitory effect of fosmidomycin on the asexual growth of Babesia bovis and Babesia bigemina (2008)
  • Author(s): Sivakumar, T., Aboulaila, M. R. A., Khukhuu, A., Iseki, H., Alhassan, A., Yokoyama, N., Igarashi, I.
  • Journal Title: J. Protozool. Res. 18 Pages: 71-78
  • Peer Reviewed
• [Journal Article] Amount of cholesterol in host membrane affects erythrocyte invasion and replication by Babesia bovis (2007)
  • Author(s): Okubo, K., Yokoyama, N., Takabatake, N., Okamura, M., Igarashi, I.
  • Journal Title: Parasitology 134 Pages: 625-30
  • Peer Reviewed
• [Journal Article] Development of Loop-Mediated Isothermal Amplification (LAMP) method for diagnosis of equine piroplasmosis (2007)
  • Author(s): Alhassan, A., Thekisoe, O. M., Yokoyama, N., Inoue, N., Motloang, M. Y., Mbati, P. A., Yin, H., Katayama, Y., Anzai, T., Sugimoto, C., Igarashi, I.
  • Journal Title: Vet. Parasitol. 143 Pages: 155-60
  • Peer Reviewed
• [Journal Article] Modification of host erythrocyte membranes by trypsin and chymotrypsin treatments and effects on the in vitro growth of bovine and equine Babesia Parasites (2007)
  • Author(s): Okamura, M., Yokoyama, N., Takabatake, N., Okubo, K., Ikehara, Y., Igarashi, I.
  • Journal Title: J. Parasitol. 93 Pages: 208-211
  • Peer Reviewed
• [Journal Article] Babesia bovis : Subcellular localization of host erythrocyte membrane components during their asexual growth (2007)
  • Author(s): Okamura, M., Yokoyama, N., Takabatake, N., Okubo, K., Ikehara, Y., Igarashi, I.
  • Journal Title: Exp. Parasitol. 116 Pages: 91-4
  • Peer Reviewed
• [Journal Article] Comparative evaluation of the sensitivity of LAMP, PCR and In vitro culture methods for diagnosis of equine piroplasmosis (2007)
  • Author(s): Alhassan, A., Govind, Y., Tam, N. T., Thekisoe, O. M. M., Yokoyama, N., Igarashi, I.
  • Journal Title: Parasitol. Res. 100 Pages: 1165-8
  • Peer Reviewed
• [Journal Article] Cyclin-dependent kinase inhibitors block erythrocyte invasion and intraerythrocytic development of Babesia bovis in vitro (2007)
  • Author(s): Nakamura, K., Yokoyama, N., Igarashi, I.
  • Journal Title: Parasitology 134 Pages: 1347-53
  • Peer Reviewed
• [Journal Article] Effects of Cysteine Protease Inhibitors on In Vitro Growth (2007)
  • Author(s): Okubo, K., Yokoyama, N., Govind, Y., Alhassan, A., Igarashi, I.
  • Journal Title: Exp. Parasitol. 117 Pages: 214-7
  • Peer Reviewed
• [Journal Article] Comparison of Polymerase chain reaction methods for the detection of Theileria equi infection using whole blood compared to pre-extracted DNA samples as PCR templates (2007)
  • Author(s): Alhassan, A., Iseki, H., Kim, C., Yokoyama, N., Igarashi, I.
  • Journal Title: Trop. Anim.Health Prod. 39 Pages: 369-74
  • Peer Reviewed
• [Journal Article] Involvement of a host erythrocyte sialic acid content in Babesia bovis infection (2007)
  • Author(s): Takabatake, N., Okamura, M., Yokoyama, N., Okubo, K., Ikehara, Y., Igarashi, I.
  • Journal Title: J. Vet. Med. Sci. 69 Pages: 999-1004
  • Peer Reviewed
• [Journal Article] Development of two immunochromatographic tests for the serodiagnosis of bovine babesiosis (2007)
  • Author(s): Kim, C., Alhassan, A., Verdida, R. A., Yokoyama, N., Xuan, X., Fujisaki, K., Kawazu, S, Igarashi, I.
  • Journal Title: Vet. Parasitol. 148 Pages: 137-43
  • Peer Reviewed
• [Journal Article] Glycophorin A-knockout mice, which lost sialoglycoproteins from the red blood cell membrane, are resistant to lethal infection of Babesia rodhaini (2007)
  • Author(s): Takabatake, N., Okamura, M., Yokoyama, N., Ikehara, Y., Akimitsu, N., Arimitsu, N., Hamamoto, H., Sekimizu, K., Suzuki, H., Igarashi, I.
  • Journal Title: Vet. Parasitol. 148 Pages: 93-101
  • Peer Reviewed
• [Journal Article] Development of Taqman-based real-time PCR assays for diagnostic detection of Babesia bovis and Babesia bigemina (2007)
  • Author(s): Kim, C., Iseki, H., Maria Shirley Herbas, Yokoyama, N., Suzuki, H., Xuan, X., Fujisaki, K., Igarashi, I.
  • Journal Title: Am. J. Trop. Med. Hyg. 77 Pages: 837-41
  • Peer Reviewed
• [Journal Article] A shared antigen among Babesia species : ribosomal phosphoprotein P0 as a universal babesial vaccine candidate (2007)
  • Author(s): Terkawi, M. A., Jia, H., Gabriel, A., Goo, Y. K., Nishikawa, Y., Yokoyama, N., Igarashi, I., Fujisaki, K., Xuan, X.
  • Journal Title: Parasitol. Res. 102 Pages: 35-40
  • Peer Reviewed
• [Journal Article] Asexual growth of Babesia Bovis is inhibited by specific sulfated glycoconjugates (2007)
  • Author(s): Bork, S., Yokoyama, N., Hashiba, S., Nakamura, K., Takabatake, N., Okamura, M., Ikehara, Y., Igarashi, I.
  • Journal Title: J. Parasitol. 93 Pages: 1501-1504
  • Peer Reviewed
• [Journal Article] Development of a Multiplex Loop-Mediated Isothermal Amplification (mLAMP) Method for the Simultaneous Detection of Bovine Babesia Parasites (2007)
  • Author(s): Iseki, H, Alhassan, A, Ohta, N., Thekisoe, O. M. M., Yokoyama, N., Inoue, N., Nambota, A., Yasuda, J., Igarashi, I.
  • Journal Title: J. Microbiol. Methods 71 Pages: 281-7
  • Peer Reviewed
• [Journal Article] Preliminary evaluation of oligomannose-coated liposome vaccines against lethal protozoan infections in mice (2007)
  • Author(s): Kuboki, N., Tiwananthagorn, W., Takagi, H., Nakayama, T., Xuan, X., Inoue, N., Igarashi, I., Tsujimura, K., Ikehara, Y., Kojima, N., Yokoyama, N.
  • Journal Title: J. Protozool. Res. 17 Pages: 9-15
  • Peer Reviewed
• [Journal Article] Seroprevalence of Babesia infections of dairy cows in northern Thailand
  • Author(s): Iseki, H., Zhou, L., Kim, C., Inpankaew, T., Sununta, C., Yokoyama, N., Xuan, X., Jittapalapong, S., Igarashi, I.
  • Journal Title: Vet. Parasitol
• [Presentation] ウシバベシア2種を同時に診断可能なプロテインアレイの開発 (2010)
  • Author(s): 玉城志緒, 井関博, 周麗佳, 小山亜紀, 横山直明, Jittapalapong Sathaporn, 五十嵐郁男
  • Organizer: 第149回日本獣医学会学術集会
  • Place of Presentation: 武蔵野
  • Year and Date: 20100326-20100328
• [Presentation] Molecular characterization of a new spherical body protein of Babesia bovis and evaluation its potential use for serodiagnosis (2009)
  • Author(s): Terkawi M. Alaa, Seuseu Junior, AbouLaila Mahmound, 横山直明, 玄学南, 五十嵐郁男
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取
  • Year and Date: 20090925-20090927
• [Presentation] バベシア原虫の赤内型増殖に関する分子生物学的研究 (日本獣医学会賞受賞講演) (2009)
  • Author(s): 横山直明
  • Organizer: 第147回日本獣医学会学術集会
  • Place of Presentation: 栃木
  • Year and Date: 20090402-20090404
• [Presentation] ヒトバベシア症の簡易迅速血清診断法の開発 (2009)
  • Author(s): 井関博, Chul-min Kim, 斉藤あつ子, 簑田友香, 猪熊壽, 横山直明, 五十嵐郁男
  • Organizer: 第147回日本獣医学会学術集会
  • Place of Presentation: 栃木
  • Year and Date: 20090402-20090404
• [Presentation] バベシア原虫における遺伝子改変技術の開発 (2009)
  • Author(s): 田中みほ, 櫻井達也, 井上昇, 横山直明, 玄学南, 河津信一郎
  • Organizer: 第147回日本獣医学会学術集会
  • Place of Presentation: 栃木
  • Year and Date: 20090402-20090404
• [Presentation] バベシア症に対するオリゴマンノース糖鎖被覆リポソームワクチンの効果 (2009)
  • Author(s): 横山直明, 石亀貴士, 高畠規之, 井関博, 太田奈保美, 小山亜紀, 五十嵐郁男, 西川義文, 池原譲, 小島直也
  • Organizer: 第147回日本獣医学会学術集会
  • Place of Presentation: 栃木
  • Year and Date: 20090402-20090404
• [Presentation] タイにおけるウシバベシア症の疫学調査 (2008)
  • Author(s): 周麗佳, 井関博, Tawin Inpankaew, 横山直明, 玄学南, Sathaporn Jittapalapong, 五十嵐郁男
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 宮崎
  • Year and Date: 20080924-20080926
• [Presentation] Growth inhibitory effect of terpene nerolidol against Babesia parasites (2008)
  • Author(s): Abou-Laila, M., Sivakumar, T., Yokoyama, N., Igarashi, I.
  • Organizer: 第156回日本獣医学会学術集会
  • Place of Presentation: 宮崎
  • Year and Date: 20080924-20080926
• [Presentation] バベシア原虫と宿主赤血球膜シアル酸分子の関わりについて (2008)
  • Author(s): 横山直明
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢
  • Year and Date: 20080515-20080514
• [Presentation] マウスバベシア原虫と宿主赤血球膜シアル酸分子の関わりについて (2008)
  • Author(s): 横山直明, 高畠規之, 井関博, 池原譲, 五十嵐郁男
  • Organizer: 第77回日本寄生虫学会大会
  • Place of Presentation: 長崎
  • Year and Date: 20080403-20080404
• [Presentation] Diagnostic real-time PCR assay for the quantitative detection of Theileria equi from equine blood samples (2008)
  • Author(s): 横山直明, Chul-min Kim, Lidia Blanco, Andy Alhassan, 井関博, 玄学南, 五十嵐郁男
  • Organizer: 第145回日本獣医学会学術集会
  • Place of Presentation: 相模原
  • Year and Date: 20080328-20080330
• [Presentation] Babesia bovis 2-Cys型ペルオキシレドキシンのクローニングと機能解析 (2008)
  • Author(s): 田中みほ, 櫻井達也, 横山直明, 井上昇, 川津信一郎
  • Organizer: 第145回日本獣医学会学術集会
  • Place of Presentation: 相模原
  • Year and Date: 20080328-20080330
• [Presentation] Involvement of host sialic acids in erythrocyte invasion of Babesia parasites (2007)
  • Author(s): Naoaki Yokoyama
  • Organizer: The 3rd International Zoonosis Seminar
  • Place of Presentation: Jeju-Do, Korea
  • Year and Date: 20071129-20071130
• [Presentation] Epoxomicin inhibits the in vitro growth of Babesia parasites (2007)
  • Author(s): Abou-Laila Mahmoud, 中村一哉, Govind Yadav, 横山直明, 五十嵐郁男
  • Organizer: 第144回日本獣医学会学術集会
  • Place of Presentation: 江別
  • Year and Date: 20070902-20070904
• [Presentation] Glycophorin Aノックアウトマウス内で増殖する Babesia rodhaini の分離 (2007)
  • Author(s): 高畠規之, 井関博, 池原譲, 関水和久, 横山直明, 五十嵐郁男
  • Organizer: 第144回日本獣医学会学術集会
  • Place of Presentation: 江別
  • Year and Date: 20070902-20070904
• [Presentation] Effects of protein kinase inhibitors on the in vitro growth of Babesia bovis (2007)
  • Author(s): Bork-Mimm, S., Das, S., Okubo, K., Yokoyama, N., Igarashi, I., Pfister, K.
  • Organizer: The 21st International Conference of the World Association for the Advancement of Veterinary Parasitology
  • Place of Presentation: Belgium
  • Year and Date: 20070819-20070823
• [Presentation] バベシア原虫と宿主赤血球膜分子の関わりについて (2007)
  • Author(s): 横山直明, 岡村雅史, 高畠規之, 太田奈保美, 池原譲, 五十嵐郁男
  • Organizer: 第15回分子寄生虫学ワークショップ
  • Place of Presentation: 草津
  • Year and Date: 20070725-20070728
• [Presentation] Effects of Cysteine Protease Inhibitors on the In Vitro Growth of Babesia bovis (2007)
  • Author(s): 大久保和洋, 横山直明, 五十嵐郁男
  • Organizer: 第143回日本獣医学会学術集会
  • Place of Presentation: つくば
  • Year and Date: 20070403-20070405
• [Remarks] ホームページ等
  • URL: http://www.obihiro.ac.jp/~protozoa/index.html