2010 Fiscal Year Final Research Report
Development of PET Imaging through Rapid Azaelectrocyclization and Oligosaccharide-Engineered Functional Macromolecules
| Project/Area Number |
19681024
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | Osaka University |
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Principal Investigator |
TANAKA Katsunori Osaka University, 大学院・理学研究科, 助教 (00403098)
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| Project Period (FY) |
2007 – 2010
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| Keywords | アザ電子環状反応 / PET / 標識化 / イメージング / 糖鎖 |
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| Research Abstract |
Efficient labeling of peptides, proteins, and antibodies were developed based on the rapid azaelectrocyclization, and the method was applied to the PET and the noninvasive fluorescence imaging. General solid-supported synthesis of N-glycans was established and their dendrimer-type clusters, mimicking the "glycan multivalency effects" in vivo, were efficiently prepared by his own "self-activating" Huisgen 1,3-dipolar cycloaddition. Modification of the proteins and/or the living cell surfaces by N-glycans were also established by rapid azaelectrocyclization. PET and noninvasive fluorescence imaging successfully visualized the trafficking of N-glycoclusters and N-glycan-engineered living cells to the specific organs and/or tumors implanted in living animals.
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[Journal Article] Positron Emission Tomography (PET) and Fluorescence Imaging of Dendrimer-type N-Glycan Clusters : Remarkable Structural Dependency of Oligosaccharides on In Vivo Dynamics2010
Author(s)
K.Tanaka, E.R.O.Siwu, K.Minami, K.Hasegawa, S.Nozaki, Y.Kanayama, K.Koyama, C.W.Chen, J.C.Paulson, Y.Watanabe, K.Fukase
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Journal Title
Angew.Chem.Int.Ed. 49
Pages: 8195-8200
Peer Reviewed
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[Journal Article] Electrocyclization-Based Labeling Allows Efficient In Vivo Imaging of Cellular Trafficking2010
Author(s)
K.Tanaka, K.Minami, T.Tahara, Y.Fujii, E.R.O.Siwu, S.Nozaki, H.Onoe, S.Yokoi, K.Koyama, Y.Watanabe, K.Fukase
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Journal Title
ChemMedChem 5
Pages: 841-845
Peer Reviewed
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[Remarks] ホームページ等
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[Remarks] 科学新聞(2010年6月4日)
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[Remarks] 読売新聞(2010年5月日)
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[Remarks] 読売新聞(HP)(2010年5月21日)
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[Remarks] 神戸新聞(HP)(2010年5月20日)
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[Remarks] 日経プレスリリース(HP)(2010年5月20日)
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[Remarks] 朝日新聞(2010年5月18日)
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[Remarks] 日経プレスリリース(HP)(2010年4月19日)
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[Remarks] 化学工業日報(2009年6月22日)
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[Remarks] (株)キシダ化学ホームページ(2008年4月1日)
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[Remarks] 理化学研究所ホームページ(2008年4月1日)
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