Single Molecule Glycomics by Nanoscale Electrophoresis

2022 Fiscal Year Final Research Report

• Project/Area Number: 19H02567
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 28040:Nanobioscience-related
• Research Institution: Kyushu University (2020-2022); Institute of Physical and Chemical Research (2019)
• Principal Investigator: Kawai Takayuki 九州大学, 理学研究院, 准教授 (60738962)
• Co-Investigator(Kenkyū-buntansha): 谷口 雄一 京都大学, 高等研究院, 教授 (90556276)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: キャピラリー電気泳動 / 一分子顕微鏡 / 糖鎖 / タンパク質

Outline of Final Research Achievements

In this research we aim to realize "single molecule glycome analysis" by coupling micro/nano-scale electrophoresis and single fluorescent molecule microscopy. Biomolecules including glycans and proteins were electrophoretically separated and migrating behavior of each molecule was observed as a movie. By analyzing their apparent electrophoretic velocity, molecular species and their condition like conjugation with another molecule were well determined.

Free Research Field

生命分析化学

Academic Significance and Societal Importance of the Research Achievements

本研究により，従来の化学分析法の感度限界がzmol (1000分子レベル) からymol (1分子レベル) まで大幅に向上し，これまで不可能だった一細胞小器官・エクソソームなどからのグライコーム解析を実現できると期待される。また本技術は糖鎖に限らず，タンパク質や核酸など蛍光標識可能なあらゆる物質に拡張可能であり，本研究が一分子計測が標準化する時代の幕開けを導く第一歩になると期待される。