Postgenomic study on postharvest physiology of carnation opened up by ethylene response factors

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K06034
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 39030:Horticultural science-related
• Research Institution: Okayama University
• Principal Investigator: HARADA Taro 岡山大学, 教育学域, 講師 (80468256)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: エチレン依存性花弁老化 / エチレン応答因子 / MAP / RNA-Seq

Outline of Final Research Achievements

In this study, the ethylene response factor (ERF) gene family in carnation was analyzed with the whole genome information. First, the ERF members that show responses to ethylene were identified by gene expression analyses. Second, involvement of an ethylene response element (ERE) in the ethylene induction of DcERF4 was investigated by promoter assays. Third, based on the knowledge that ERF is an important factor in oxygen sensing mechanism in plant, experimental systems for modified atmosphere packaging (MAP) of carnation florets were established, and differentially expressed genes (DEG) during ethylene-dependent petal senescence and during preservation of florets under MAP were analyzed and compared by RNA-Seq.

Free Research Field

花き園芸学、植物生理学

Academic Significance and Societal Importance of the Research Achievements

カーネーションは，世界および国内における主要花き品目の1つであり，我が国の研究者がその研究を牽引してきたが，2013年の全ゲノム解読完了以降，その情報を利用した新たな研究が期待されている．本研究では，園芸学的に重要な花の老化過程における新たな研究対象となり得る遺伝子を提示するとともに，実用化が期待される切り花のMAP技術の効果に関する生理学的，分子生物学的根拠を提示することで，カーネーションの収穫後生理研究の新たな次元の開拓のための基盤を構築した．