2021 Fiscal Year Final Research Report

Study of the inhibitory effects of polyphenol loaded titania nanotubes on bacterial growth and biofilm formation

Research Project

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Project/Area Number	19K10132
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 57030:Conservative dentistry-related
Research Institution	The University of Tokushima
Principal Investigator	OZAKI Kazumi 徳島大学, 大学院医歯薬学研究部(歯学域), 教授 (90214121)
Co-Investigator(Kenkyū-buntansha)	湯本浩通徳島大学, 大学院医歯薬学研究部(歯学域), 教授 (60284303) 細川義隆徳島大学, 病院, 講師 (90346601) 細川育子徳島大学, 大学院医歯薬学研究部(歯学域), 助教 (50707908)
Project Period (FY)	2019-04-01 – 2022-03-31
Keywords	バイオフィルム / 二酸化チタン / チタニアナノチューブ / ポリフェノール
Outline of Final Research Achievements	The aim of this study was to evaluate the inhibitory effect of polyphenol loaded titania nanotubes (TNT-PolyP mixture) on bacterial growth and biofilm formation. After preparing a TNT-PolyP mixture, the Streptococcus mutans UA159 strain was cultured for 12 hours using a sucrose-added BHI medium and apatite pellets supplemented with the TNT-PolyP mixture. As a result, a certain degree of antibacterial effect and biofilm formation inhibitory effect were observed. TEM observation revealed that the TNT-PolyP mixture suppresses the biofilm formation of S.mutans. In addition, it showed a tendency to reduce the roughening of the surface of apatite pellets, that is, the decalcification effect. From the above, it was shown that the TNT-PolyP mixture prepared in this study may be useful for suppressing the onset of caries.
Free Research Field	保存治療系歯学
Academic Significance and Societal Importance of the Research Achievements	う蝕や歯周病の発症要因となるだけでなく，誤嚥性肺炎など死に至る疾患の原因ともいわれる口腔バイオフィルムの駆逐技術を開発することは，歯科臨床上非常に意義深い。とくに口腔衛生管理の手段として患者に指導するブラッシングなど口腔バイオフィルムの機械的除去に加え，本研究で調製した合剤のような新たなコンセプトに基づく口腔バイオフィルム形成制御技術が応用されることで，機能障害の有無を問わず高度なブラッシングを要求しがたい高齢者や要介護者の口腔衛生管理の質が向上し，根面う蝕の発症予防や進行抑制に貢献すると考えられる。