2020 Fiscal Year Final Research Report
a novel culture method for efficient differentiation of insulin producing cells (IPC) focusing on cell polarity.
Project/Area Number |
19K18056
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 55010:General surgery and pediatric surgery-related
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Research Institution | The University of Tokushima |
Principal Investigator |
IWAHASHI Shuichi 徳島大学, 大学院医歯薬学研究部(医学域), 徳島大学専門研究員 (30531751)
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Project Period (FY) |
2019-04-01 – 2021-03-31
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Keywords | インスリン分泌細胞 / 細胞極性 |
Outline of Final Research Achievements |
We assumed that electrical stimulation could enhance cell adhesion and activation of intracellular signal pathways to promote efficiently differentiation of insulin-producing cells (IPCs) for boost up of cell function. The stimulating condition (100 Hz, 200 mV for 72 hours) was determined by previous report, but the number of survival cells significantly decreased. Then, the conditions were changed to mild condition of 1 V / cm, 0.1 ms, 55 pps reported by another previous report. However, electrical stimulation induced excessive ER stress on the cells, and reduced secretion of insulin and expression of ZO-1, which is cell adhesion protein.Electrical stimulation could not enhance differentiation of IPC under this condition. Further investigation is needed for appropriate condition.
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Free Research Field |
再生医療(内分泌細胞の効果的分化誘導法の確立)
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Academic Significance and Societal Importance of the Research Achievements |
研究代表者はこれまでに間葉系幹細胞(MSC)から膵β細胞様の機能を持つInsulin producing cell(IPC)への分化誘導実験に着手し、Histone deacetylase inhibitorを添加した2 step protocolを確立している。一方で電気刺激が細胞に与える影響として抗細胞死効果や、他細胞への分化が起こることが報告されている。電気刺激の適切な条件を同定することにより、胚葉転換のメカニズム解明やIPCへの効率的な分化誘導が可能となり得ると考えられる。
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