2022 Fiscal Year Final Research Report
Exploration of novel regulatory mechanisms of translation in bacteria
| Project/Area Number |
19K22715
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 57:Oral science and related fields
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| Research Institution | Kagoshima University (2020-2022)
Osaka University (2019) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
住友 倫子 大阪大学, 大学院歯学研究科, 講師 (50423421)
山口 雅也 大阪大学, 大学院歯学研究科, 准教授 (00714536)
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| Project Period (FY) |
2019-06-28 – 2023-03-31
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| Keywords | レンサ球菌 / 翻訳効率 / エンドリボヌクレアーゼ |
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| Outline of Final Research Achievements |
The human pathogen Streptococcus pyogenes modulates virulence in response to ambient temperature during the infection process. We searched for bacterial factors that alter translation efficiency in response to temperature shifts. Our findings suggested that not only stem-loop structures located near mRNA translation initiation sites, but also mRNA structures in 5´-untranslated regions are likely involved in the temperature-sensitive translational regulation. In addition, our data revealed that the endoribonuclease RNase Y is crucial for pilus production from strains possessing a specific transcriptional regulator.
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| Free Research Field |
細菌学
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| Academic Significance and Societal Importance of the Research Achievements |
化膿レンサ球菌はヒトに多様な疾患を引き起こす．上市されているワクチンはなく，感染者数の増加が懸念されている．したがって，ワクチン抗原の選択や治療法開発の礎となる病態発症機序の解明が求められる．体内と比較して温度が低い皮膚や咽頭などの初発感染部位から体内へ感染が拡大する際，本菌は環境温度の変化に対応して，様々な因子の発現量を調節すると考えられている．感染過程における細菌の温度感知機構を理解することにより，細菌因子発現部位の予測や治療標的・ワクチン抗原を選択する際の指標となり得る.
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