Endothelium function under limb ischemia condition of aging mouse model

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K23829
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund
• Review Section: 0802:Biomedical structure and function and related fields
• Research Institution: Kyushu University
• Principal Investigator: KAWAKUBO Eiske 九州大学, 医学研究院, 共同研究員 (50644754)
• Project Period (FY): 2019-08-30 – 2021-03-31
• Keywords: 加齢 / BubR1 / eNOS / 下肢虚血

Outline of Final Research Achievements

We tried to reveal the relation between BubR1 expression and eNOS expression or NO production, using mouse ischemic limb model. In this study, We estimated mouse ischemic limb model of BuBR1 normal expression mouse and BubR1 low expression mouse. In BubR1 low expression mouse, the recovery of limb blood perfusion after limb ischemia was less than in BubR1 normal expression mouse. This result suggested that BubR1 low expression, which was coursed by aging, inhibits angiogenesis. This is the basic of further study to reveal the relation of BubR1 and eNOS or NO.

Free Research Field

医学

Academic Significance and Societal Importance of the Research Achievements

老化と動脈硬化に関連するBubR1およびeNOSに関する研究の足がかりとなった。下肢虚血肢モデルマウスを確立し、BubR1の発現量が下肢虚血後の血管新生に関連することを確認し、細胞周期関連遺伝子BubR1が血管内皮機能と関連するメカニズムを解明する一歩となり得る。より詳細な分子学的検討を追求していくための基盤ができたと考えられる。