2010 Fiscal Year Final Research Report

Functional Analysis of auxin and microRNA in organ formation during rice embryogenesis

Research Project

Project/Area Number	20570034
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Plant molecular biology/Plant physiology
Research Institution	Nagoya University
Principal Investigator	SAZUKA Takashi Nagoya University, 生物機能開発利用研究センター, 准教授 (70362291)
Co-Investigator(Renkei-kenkyūsha)	KITANO Hidemi 名古屋大学, 生物機能開発利用研究センター, 教授 (50144184)
Project Period (FY)	2008 – 2010
Keywords	イネ / 胚発生 / 器官形成 / オーキシン / マイクロRNA
Research Abstract	The three rice mutants, club-shaped embryo1 (cle1), club-shaped embryo3 (cle3), tryptphan deficient dwarf1 (tdd1) were used to study the regulation for organ differentiation in rice embryogenesis by auxin and miRNA. The embryonic phenotype of two mutants, cle1 and cle3, are club-shaped, and that of tdd1 is globular ; the three mutants are defective for the organ formation during embryogenesis. The positional cloning indicates that the responsible genes for cle1 and tdd1 are OSDCL1 and ASB1, respectively. The detailed analysis revealed the important regulation by auxin and miRNA for rice embryogenesis. The other two mutants, cre3 and tdd1, were also analyzed.

(5 results)