Study in the effects of IBM-BMT using parabiotic mice

2010 Fiscal Year Final Research Report

• Project/Area Number: 20590413
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Kansai Medical University
• Principal Investigator: INABA Muneo Kansai Medical University, 医学部, 准教授 (70115947)
• Co-Investigator(Kenkyū-buntansha): HISHA Hiroko 関西医科大学, 医学部, 講師 (90151422) ; MAKI Masahiko 関西医科大学, 医学部, 講師 (80297001)
• Project Period (FY): 2008 – 2010
• Keywords: 並体結合マウス / 骨髄移植 / 樹状細胞 / 造血幹細胞 / 循環動態

Research Abstract

We have prepared the parabiotic mice to compare the efficiency of donor dell engraftment by IBM-BMT with that by IV-BMT in the physiological condition. BMCs from GFP mice were transplanted into C57BL/6 mice by IBM-BMT ([GFP->C57BL/6]^<IBM-BMT>), and the parabiotic mouse was made up of the ([GFP->C57BL/6]^<IBM-BMT> mouse and normal C57BL/6 mouse ([GFP→C57BL/6]^<IBM-BMT>+C57BL/6). In this mouse, the frequency of hemopoietic progenitor cells, defined as Lin^-/c-kit^+ cells, was significantly higher than that determined in the ([GFP→C57BL/6]^<IV-BMT>+C57BL/6 mouse, indicating the efficiency of donor cell engraftment by IBM-BMT.

Research Products

• [Journal Article] Interferon-□ and interleukin-12 are induced, respectively, by doublestranded DNA and single-stranded RNA in human myeloid dendritic cells. (2011)
  • Author(s): Katashiba Y., Miyamoto R., Hyo A., Shimamoto K., Murakami N., Ogata M., Amakawa R., Inaba M., Nomura S., Fukuhara S., Ito T.
  • Journal Title: Immunology 132 Pages: 165-173
  • Peer Reviewed
• [Journal Article] Defects in cytokine-mediated neuroprotective glial responses to excitotoxic hippocampal injury in senescence-accelerated mouse. (2011)
  • Author(s): Hasegawa-ishii S., Takeii S., Inaba M., Umegaki H., Chiba Y., Furukawa A., Kawamura N., Hosokawa M., Shimada A.
  • Journal Title: Brain Behav.Immunol. 25 Pages: 83-100
  • Peer Reviewed
• [Journal Article] The role of dendritic cell subsets in 2,4,6-trinitrobenzene sulfonic acid-induced ileitis. (2010)
  • Author(s): Hoshino S.,Inaba M., Iwai H., Ito T., Li M., Gershwin M. E., Okazaki K., Ikehara S.
  • Journal Title: J.Autoimmunity 34 Pages: 380-389
  • Peer Reviewed
• [Journal Article] Amelioration of cognative ability in senescence-accelerated mouse prone 8(SAMP8) by intra-bone marrow-bone marrow transplantation. (2009)
  • Author(s): Li M., Inaba M., Guo K., Abraham N. G., Ikehara S.
  • Journal Title: Neuroscience Letters 465 Pages: 36-40
  • Peer Reviewed
• [Journal Article] Preferential expansion of human umbilical cord blood-derived CD34-positive cells on major histocompatibility complex-matched amnion-derived mesenchymal stem cells. (2009)
  • Author(s): Mizokami T., Hisha H., Okazaki S., Takaki T., Wang X., Song, C., Li Q., Kato J., Hosaka N., Inaba M., Kanzaki H., Ikehara S.
  • Journal Title: Haematologica-The hematology Journal 94 Pages: 618-628
  • Peer Reviewed
• [Journal Article] Contribution of neural cell adhesion molecule (NCAM) to hemopoietic system in monkeys. (2008)
  • Author(s): Kato J., Hisha H., Wang X.-I., Mizokami T., Okazaki S., Li Q., Song C., Maki M.,Hosaka N., Adachi Y., Inaba M., Ikehara S.
  • Journal Title: Ann.Hematol. 87 Pages: 797-807
  • Peer Reviewed
• [Journal Article] Subcutaneous adipose tissue-derived stem cells facilitate colonic mucosal recovery from 2,4,6-trinitro benzene sulfonic acid (TNBS)-induced colitis in rats. (2008)
  • Author(s): Ando Y., Inaba M., Sakaguchi Y., Tsuda M., Guo K, Omae M., Okazaki K., Ikehara S.
  • Journal Title: Inflammatory Bowel Diseases 14 Pages: 826-838
  • Peer Reviewed
• [Journal Article] Long-term maintenance of donorderived hemopoiesis by intra-bone marrow-bone marrow transplantation. (2008)
  • Author(s): Omae M., Inaba M., Sakaguchi Y., Tsuda M., Miyake T., Fukui J., Iwai H., Yamashita T., Ikehara S.
  • Journal Title: Stem Cells and Development 17 Pages: 291-302
  • Peer Reviewed
• [Journal Article] Prevention of graft-versus-host disease by intrabone marrow injection of donor T cells : involvement of bone marrow stromal cells. (2008)
  • Author(s): Miyake T., Inaba M., Fukui J., Ueda Y., Hosaka N., Kamiyama Y., Ikehara S.
  • Journal Title: Clin.Exp.mmunol. 152 Pages: 153-162
  • Peer Reviewed
• [Journal Article] Long-term donor-specific tolerance in rat cardiac allografts by intrabone marrow injection of donor bone marrow cells. (2008)
  • Author(s): Guo K., Inaba M., Li M., An J., Cui W., Song C., Wang J., Cui Y., Sakaguchi Y., Tsuda M., Omae M., Ando Y., Li Q., Wang X., Ikehara S.
  • Journal Title: Transplantation. 85 Pages: 93-101
  • Peer Reviewed
• [Presentation] The role of dendritic cell subsets in 2,4,6-trinitrobenzene sulfonic acidinduced ileitis. (2010)
  • Author(s): Inaba M., Hoshino S., Okazaki K., Ikehara S.
  • Organizer: 11^<th> Annual International Symposium on Dendritic Cells "DC2010 : Forum on Vaccine Science"
  • Place of Presentation: Lugano, Switzerland
  • Year and Date: 20100926-20100930
• [Presentation] The role of dendritic cell subsets in 2,4,6-trinitrobenzene sulfonic acidinducedileitis. (2010)
  • Author(s): Inaba M., Hoshino S., Iwai H., Ito T., Li M., Gershwin M. E., Okazaki K., Ikehara S.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸ポートピアホテルおよび神戸国際展示場(神戸コンベンションセンター), Kobe, Japan
  • Year and Date: 20100822-20100827