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2010 Fiscal Year Final Research Report

Analysis of Uropathogenic-specific protein and development of the mucosal vaccine by using of USP

Research Project

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Project/Area Number 20590436
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

KURAZONO Hisao  Obihiro University of Agriculture and Veterinary Medicine, 畜産学部, 教授 (90186487)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Shingo  兵庫医科大学, 医学部, 教授 (80322741)
YAMASAKI Eiki  帯広畜産大学, 畜産学部, 助教 (40514708)
Project Period (FY) 2008 – 2010
Keywords病原性 / 粘膜ワクチン
Research Abstract

Uropathogenic-specific protein (USP) is one of the important virulence factors in UPEC. We established a highly-sensitive bead-ELISA system against USP. The minimum detection concentration of USP in the sample is 100 pg/ml. By using of this detection system, we try to clarify the relationship between the production of USP in UPEC and the symptoms of the patients suffered from UPEC infections.

  • Research Products

    (36 results)

All 2011 2010 2009 2008 Other

All Journal Article (23 results) Presentation (12 results) Remarks (1 results)

  • [Journal Article] Helicobacter pylori VacA reduces cellular expression of STAT3 and pro-survival Bcl-2 family proteins, Bcl-2 and Bcl-X_L, leading to apoptosis in gastric epithelial cells.2011

    • Author(s)
      Matsumoto A, Kurazono H, Kohno S
    • Journal Title

      Digestive Diseases and Science 56

      Pages: 999-1006

  • [Journal Article] Hybrid & El Tor variant biotypes of Vibrio cholerae Ol in Thailand2011

    • Author(s)
      Na-Ubol M, Kurazono H, Chaicumpa W
    • Journal Title

      Indian J.Med.Res. 133

      Pages: 387-394

  • [Journal Article] Double short-time exposure to pirarubicin produces higher cytotoxity against T24 bladder cancer cells.2011

    • Author(s)
      Maruyama T, Yamamoto S, Shima H
    • Journal Title

      J.Infect.Chemother. 17

      Pages: 11-16

  • [Journal Article] A single-dose regimen for antimicrobial prophylaxis to prevent perioperative infection in urological clean and clean-contaminated surgery.2011

    • Author(s)
      Higuchi Y, Takesue Y, Yamamoto S
    • Journal Title

      J.Infect.Chemother. 17

      Pages: 219-222

  • [Journal Article] A V_HH that neutralizes the zinc-metalloproteinase activity of botulinum neurotoxin type A.2010

    • Author(s)
      Thanongsaksrikul J, Kurazono H, Chaicumpa W
    • Journal Title

      J.Biol.Chem. 285

      Pages: 9657-9666

  • [Journal Article] Helicobacter pylori CagA Inhibits Endocytosis of Cytotoxin VacA in Host Cells.2010

    • Author(s)
      Akada KJ, Kurazono H, Nakamura K
    • Journal Title

      Disease Models & Mechanisms 3

      Pages: 605-617

  • [Journal Article] Far-infrared rays control prostate cancer cells in vitro and in vivo.2010

    • Author(s)
      Shima H, Yamamoto S
    • Journal Title

      Cent.Eur.J.Biol. 5

      Pages: 178-189

  • [Journal Article] Prognostic of Japanese metastatic renal cell carcinoma (mRCC) patients in the cytokine era-a cooperative group report of 1,463 mRCC patients.2010

    • Author(s)
      Naito S, Yamamoto S, Tomita Y
    • Journal Title

      Eur.Urol. 57

      Pages: 317-315

  • [Journal Article] A transient increase of calcineurin phosphatase activity in living-donor kidney transplant recipient with acute rejection.2010

    • Author(s)
      Fukudo M, Yamamoto S, Inui K
    • Journal Title

      Drug Metab.Pharmacokinet 25

      Pages: 411-417

  • [Journal Article] The antitumour effect of γδ T Cells is enhanced by valproic acid-induced up-regulation of NKG2D ligands.2010

    • Author(s)
      Suzuki T, Terao S, Acharya B, Naoe M, Yamamoto S, Okamura H, Gotoh A
    • Journal Title

      Anticancer Res. 30

      Pages: 4509-4514

  • [Journal Article] Direct binding of gangliosides to Helicobacter pylori vacuolating cytotoxin (VacA) neutralizes its toxin activity.2010

    • Author(s)
      Wada A, Yamasaki E, Senba M
    • Journal Title

      Glycobiology 20

      Pages: 668-678

  • [Journal Article] Helicobacter pylori VacA-induced inhibition of GSK3 through the PI3K/Akt signaling pathway.2009

    • Author(s)
      Nakayama M, Yamasaki E, Kurazono H, Hirayama T
    • Journal Title

      J.Biol.Chem. 284

      Pages: 1612-1619

  • [Journal Article] Genetic profiles of fluoroquinolone-resistant Escherichia coli isolated from cystitis : phylogeny, virulence factors, PAlusp-subtypes, and mutation patterns.2009

    • Author(s)
      Takahashi A, Kurazono H, Yamamoto S
    • Journal Title

      J.Clin.Microbiol. 47

      Pages: 791-795

  • [Journal Article] Human monoclonal ScFv neutralize lethal Thai cobra, Naja kaouthia, neurotoxin.2009

    • Author(s)
      Kulkeaw K, Kurazono H, Chaicumpa W
    • Journal Title

      J.Proteomics 72

      Pages: 270-282

  • [Journal Article] Clinical efficacy of oral administration of 200 mg gatifloxacin once daily for 3 days for the treatment of patients with uncomplicated cystitis.2009

    • Author(s)
      Yamamoto S
    • Journal Title

      J.Infect.Chemother. 15

      Pages: 104-107

  • [Journal Article] Diagnostic accuracy of volume-rendered computed tomography movie and other computed tomography-based imaging methods in assessment of renal vascular anatomy for laparoscopic donor nephrectomy.2009

    • Author(s)
      Yamamoto S
    • Journal Title

      Int.Urol.Nephrol. 41

      Pages: 785-790

  • [Journal Article] Basic fibroblast growth factor causes urinary bladder overactivity trough gap junction generation in the smooth muscle.2009

    • Author(s)
      Imamura M, Yamamoto S, Ogawa O
    • Journal Title

      Am.J.Pysiol.Renal Physiol. 297

      Pages: F46-F54

  • [Journal Article] Molecular characterization of Helicobacter pylori VacA-induction of IL-8 in U937 cells reveals a prominent role for p38MAP kinase in ATF-2, CREB and NF K B activation.2008

    • Author(s)
      Hisatsune J, Kurazono H, Yamasaki E
    • Journal Title

      J.Immunol. 180

      Pages: 5017-5027

  • [Journal Article] Molecular typing of Vibrio cholerae O1 isolates from Thailand by pulsed-field gel electrophoresis.2008

    • Author(s)
      Tapchaisri P, Kurazono H, Chaicumpa W
    • Journal Title

      J.Health Popul.Nutr. 26

      Pages: 79-87

  • [Journal Article] 21-hydroxylase deficiency presenting as an adrenal incidentaloma : Endocrinological evaluation and mutational analysis.2008

    • Author(s)
      Takahashi S, Yamamoto S
    • Journal Title

      Endocrinologist 18

      Pages: 207-210

  • [Journal Article] Diagnostic efficacy of free to total ratio of prostate-specific antigen and prostate-specific antigen velocity, singly and in combination, in detecting prostate cancer in patients with total serum prostate-specific antigen between 4 and 10 ng/ml.2008

    • Author(s)
      Yamamoto S
    • Journal Title

      Int.Urol.Nephrol. 40

      Pages: 85-89

  • [Journal Article] Antibiotic prophylaxis for transrectal prostate biopsy : a prospective randomized study of tosufloxacin versus levofloxacin.2008

    • Author(s)
      Yamamoto S
    • Journal Title

      Int J Urol 15

      Pages: 604-606

  • [Journal Article] Salvage therapy trial for erectile dysfunction using phosphodiestrase type 5 inhibitors and vitamin E : Preliminary report.2008

    • Author(s)
      Kondoh N, Yamamoto S
    • Journal Title

      Aging Male 11

      Pages: 167-170

  • [Presentation] on behalf of the Japanese UTI Research Group : A randomized clinical trial to evaluate the preventive effect of cranberry juice (UR65) for patients with recurrent urinary tract infection.2011

    • Author(s)
      Takahashi S, Hamasuna R, Yasuda M, Arakawa S, Tanaka K, Ishikawa K, Kiyota H, Hayami H, Yamamoto S, Matsumoto T
    • Organizer
      ICC/ECCMID 2011
    • Place of Presentation
      Milan, Italy
    • Year and Date
      20110507-20110510
  • [Presentation] Assessment of a single-dose regimen for antimicrobial prophylaxis to prevent perioperative infection in urologic surgery.2010

    • Author(s)
      Yamamoto S
    • Organizer
      German-Japanese Confederation of Urology 9th Scientific Meeting.
    • Place of Presentation
      Munster, Germany
    • Year and Date
      20100619-20100626
  • [Presentation] Enhancement of γδ T cell antitumor activity against prostate cancer cell through Toll-like receptor 2 and 3 ligand dependent activation.2010

    • Author(s)
      Suzuki T, Tacharya B, Terao S, Noj ima M, Yamamoto S, Gotoh A
    • Organizer
      AUA Annual Meeting '10
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      20100529-20100603
  • [Presentation] 尿路病原性大腸菌の産生するUSPに対する特異的同定法の開発2010

    • Author(s)
      山崎栄樹、高橋彰、山本新吾、星英之、牧野壮一、倉園久生
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      パシフィコ横浜、横浜市
    • Year and Date
      20100327-20100329
  • [Presentation] Analysis of Pthogenicity island of Uropathogenic Escherichia coli which encodes the USP.2010

    • Author(s)
      倉園久生倉園久生
    • Organizer
      SPU Forum : Updates on Common Viral and Bacterial Infections.
    • Place of Presentation
      Universiti Malaysia Sabah, School of Medicine
    • Year and Date
      2010-10-25
  • [Presentation] Assessment of a single-dose regimen for antimicrobial prophylaxis to prevent perioperative infection in urologic surgery.2010

    • Author(s)
      Higuchi Y, Yamada Y, Ueda Y, Suzuki Y, Aihara K, Maruyama T, Nojima M, Yamamoto S
    • Organizer
      Society for Infection and Inflammation (SIIU) at AUA Annual Meeting '10.
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2010-05-30
  • [Presentation] Diversity in the expression of Salmonella enterotoxin that has a cytotoxic effect on cultured cells by various Salmonella species.2009

    • Author(s)
      Yamasaki E, Nakano M, Hoshi H, Seto Y, Chongsa-nguan M, Chicumpa W, Nair G B, Makino S-I, Hirayama T, Kurazono H
    • Organizer
      3rd ASM Conference on Salmonella : Biology, Pathogenesis & Prevention.
    • Place of Presentation
      Aix-en-Provence, France
    • Year and Date
      20091005-20091009
  • [Presentation] Transient disappearance of donor-specific antibody on post-transplant day 1 may indicate high risk for antibody-mediated acute rejection following kidney transplantation in sensitized recipients.2009

    • Author(s)
      Ueda Y, Nojima M, Hashimoto M, Higuchi Y, Kinoshita T, Hao H, Kyo M, Ichikawa Y, Hirota S, Yamamoto S, Shima H
    • Organizer
      European Society for Organ Transplantation 2009
    • Place of Presentation
      Paris, France
    • Year and Date
      20090827-20090830
  • [Presentation] The phylogenetic and virulence profiles of quinolone-resistant Escherichia coli isolated from cystitis are different from quinolone-susceptible counterparts.2009

    • Author(s)
      Yamamoto S, Takahashi A, Muratani T, Yasuda M, Takahashi S, Monden K, Ishikawa K, Kiyota H, Arakawa S, Matsumoto T, Shima H, Kurazono H
    • Organizer
      AUA Annual Meeting '09
    • Place of Presentation
      Chicago, USA
    • Year and Date
      20090426-20090430
  • [Presentation] The antitumor effect of y6T cells against bladder cancer cells is enhanced following upregulation of NKG2D ligands by valproic acid.2009

    • Author(s)
      Foru Suzuki, Bishnu Acharya, Shuji Terao, Shingo Yamamoto, Hiroki Shima, Haruki Okamura, Akinobu Gotoh
    • Organizer
      AUA Annual Meeting '09
    • Place of Presentation
      Chicago, USA
    • Year and Date
      20090426-20090430
  • [Presentation] Biological activities of Salmonella enterotoxin.2008

    • Author(s)
      Yamasaki E, Hoshi H, Seto Y, Chongsa-nguan M, Chicumpa W, G B Nair, Makino S-I, Hirayama T, Kurazono H
    • Organizer
      The 43rd Joint Conference US-Japan Cooperative Medical Science Program, Cholera Panel
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      20081117-20081119
  • [Presentation] AAUS Symposium : Catheter Associated UTI : CAUTI European and Asian guidelines.2008

    • Author(s)
      Yamamoto S
    • Organizer
      Pre-congress meeting of the 5th Asian Association of UTI and STD
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2008-11-29
  • [Remarks] ホームページ等

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Published: 2012-01-26   Modified: 2016-04-21  

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