2010 Fiscal Year Final Research Report
Identification of novel autoantibodies against cerebral cell surface membrane proteins as biological markers of multiple sclerosis
| Project/Area Number |
20590992
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Gifu University |
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Principal Investigator |
KIMURA Akio Gifu University, 医学部附属病院, 助教 (00362161)
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| Co-Investigator(Kenkyū-buntansha) |
INUZUKA Takashi 岐阜大学, 医学系研究科, 教授 (50184734)
HOZUMI Isao 岐阜大学, 医学系研究科, 准教授 (20242430)
TANAKA Yuji 岐阜大学, 医学系研究科, 助教 (70377654)
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| Project Period (FY) |
2008 – 2010
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| Keywords | 脳・神経 / 免疫学 |
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| Research Abstract |
We identified the autoantibody against Phosphoglycerate mutase 1 (PGAM 1) and mitochondrial heat shock protein 70 (mtHSP70) in serum from multiple sclerosis (MS) patients by immunoproteomic approach. We tested more samples by one-dimensional immunoblotting of human recombinant PGAM1 and mtHSP70. The prevalence of the anti-PGAM 1 antibody is significantly higher in serum from MS patients (81%) than in serum from other neurological diseases (ONDs) and healthy controls (HCs) (24～32%) (p<0.05). The prevalence of the anti-mtHSP70 antibody is also significantly higher in serum from MS patients (68%) than in serum from ONDs and HCs (20～30%) (p<0.05). We studied the clinical features and magnetic resonance imaging findings of MS patients with these antibodies. As a result, there were no significant differences between these antibodies-positive and -negative MS patients. Additionally, in our comprehensive analysis of the prevalence of both the anti-PGAM1 antibody and the anti-mtHSP70 antibody, the positivity rates of both these antibodies were significantly higher in serum from MS patients (57%) than in serum from patients with ONDs and HCs (0～16%) (p<0.05) ; moreover, the specificity of this combination assay was higher than that of the assay of only one antibody (57% sensitivity ; 93% specificity). Results of our study suggest that the combination of both these antibodies is useful for a more specific diagnosis of MS.
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[Journal Article] Antibodies in Patients With Neuropsychiatric Systemic Lupus Erythematosus.2010
Author(s)
Kimura A, Kanoh Y, Sakurai T, Koumura A, Yamada M, Hayashi Y, Tanaka Y, Hozumi I, Takemura M, Seishima M, Inuzuka T
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Journal Title
Neurology 74
Pages: 1372-1379
Peer Reviewed
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[Journal Article] Antibodies to the GABAB receptor in limbic encephalitis with seizures : case series and characterization of the antigen.2010
Author(s)
Lancaster E, Lai M, Peng X, Hughes E, Constantinescu R, Raizer J, Friedman D, Skeen MB, Grisold W, Kimura A, Ohta K, Iizuka T, Guzman M, Graus F, Moss SJ, Balice-Gordon R, Dalmau J
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Journal Title
Lancet Neurol. 9
Pages: 67-76
Peer Reviewed
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[Journal Article] Progressive multifocal leukoencephalopathy and CD4+ T-lymphocytopenia in a patient with Sjogren syndrome.2008
Author(s)
Hayashi Y, Kimura A, Kato S, Koumura A, Sakurai T, Tanaka Y, Hozumi I, Sunden Y, Orba Y, Sawa H, Takahashi H, Inuzuka T
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Journal Title
J Neural Sci. 268
Pages: 195-198
Peer Reviewed
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[Journal Article] Selective cauda equine hypertrophy with idiopathic inflammation.2008
Author(s)
Hayashi Y, Sakurai T, Kimura A, Ikeda T, Matsuyama Z, Suzuki Y, Tanaka Y, Hozumi I, Hosoe H, Takahashi H, Inuzuka T
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Journal Title
Muscle Nerve. 38
Pages: 1065-1069
Peer Reviewed
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