2022 Fiscal Year Final Research Report
Development of a method for inducing differentiation of pluripotent stem cells into taste cells; using taste bud organoid co-cultures
| Project/Area Number |
20K10005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 57040:Regenerative dentistry and dental engineering-related
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| Research Institution | Kyoto University (2021-2022)
Kansai Medical University (2020) |
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Principal Investigator |
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| Project Period (FY) |
2020-04-01 – 2023-03-31
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| Keywords | 味蕾オルガノイド / 分化誘導 / 多能性幹細胞 / 味覚再生 |
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| Outline of Final Research Achievements |
Taste cells are responsible for the sense of taste, one of the five senses. There has been no report of induction of differentiation of taste cells from pluripotent stem cells. In a previous study, the applicant generated differentiated cells expressing taste cell markers from mouse ES cells. In the present study, Sox2 was forced to be expressed in a differentiation stage-specific manner using a drug-inducible Tet-on system in order to increase the efficiency of differentiation induction. We were also able to confirm Sox2 expression by a fluorescent reporter and generated a larger number of cells positive for the gustatory cell marker than ever before.
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| Free Research Field |
再生医療
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| Academic Significance and Societal Importance of the Research Achievements |
細胞レベルの味覚研究を行うには大量の味細胞が必要となる。今まではマウスやサルなどの実験動物から味蕾オルガノイドを作製していたが、分裂回数や供給量には限界がある。多能性幹細胞から味細胞を分化誘導できれば、味覚研究全般が大幅に進展すると考えられる。社会実装できる可能性も高い。本研究ではマウスES細胞より効率的に味細胞マーカー陽性細胞を作製できた。これは、ヒトES細胞やヒトiPS細胞にも応用できると考えられる。
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