2023 Fiscal Year Final Research Report

Distribution of periodontopathogenic bacteria in major systemic blood vessel walls - based on a case of death due to cerebrovascular accident

Research Project

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Project/Area Number	20K10287
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 57080:Social dentistry-related
Research Institution	Hokkaido University
Principal Investigator	Takeuchi Akiko 北海道大学, 医学研究院, 特任助教 (00867179)
Co-Investigator(Kenkyū-buntansha)	的場光太郎北海道大学, 医学研究院, 教授 (00466450) 箕輪和行北海道大学, 歯学研究院, 教授 (30209845) 兵頭秀樹福井大学, 学術研究院医学系部門, 教授 (30306154) 亀田浩之北海道大学, 歯学研究院, 助教 (70829887) 長谷部晃北海道大学, 歯学研究院, 教授 (90281815)
Project Period (FY)	2020-04-01 – 2024-03-31
Keywords	歯周病原菌 / 死後画像 / PCR / P. gingivalis
Outline of Final Research Achievements	We examined periodontal bacteria in the oral cavity and their effects on major systemic blood vessels. Bacteria were collected from the oral cavity of forensic autopsies, and periodontal bacteria were identified by PCR testing. In 77.3% of the cases, multiple periodontal pathogens were detected. Porphyromonas gingivalis (P.g) was found in 50% of the cases in which periodontopathic bacteria were detected. Comparison of the presence of P.g in the oral cavity with the calcified areas of major large blood vessels extracted by postmortem whole-body CT showed that the presence of P.g in the oral cavity was associated with more calcified areas in the large blood vessels throughout the body, suggesting a high likelihood of vascular atherosclerosis. Detection of P.g within the walls of these vessels was difficult, and the distribution of its presence within the vessel walls could not be verified.
Free Research Field	社会歯科学
Academic Significance and Societal Importance of the Research Achievements	慢性炎症としての歯周病は、口腔内に存在する歯周病原菌による細菌感染により起こり、糖尿病や心・冠動脈疾患などのさまざまな全身疾患のリスク因子と考えられている。歯周病原菌の一種であるPorphyromonas gingivalis（P.g）は、血管内皮に付着することから、アテローム性動脈硬化との関連が考えられている。今回、口腔内におけるP.gの存在と全身主要血管における石灰化領域の比較から、歯周病原菌（P.g）と血管の動脈硬化の程度に正の相関があることが示唆された。ただし、血管壁におけるP.g存在分布の比較として有意な結果は得られず、今後さらなる検討が必要と考えられた。