• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2011 Fiscal Year Final Research Report

DEVELOPMENT OF A TIME-DEPENDENT LIQUID MICRO-JET SPECTROSCOPY FOR RADIATION-INDUCED ANORMALY IN MIMETIC BIO-CELL MOLECULES

Research Project

  • PDF
Project/Area Number 21241017
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

UKAI Masatoshi  東京農工大学, 大学院・工学研究院, 教授 (80192508)

Co-Investigator(Kenkyū-buntansha) YOKOYA Akinari  日本原子力研究開発機構, 先端基礎研究センター, 研究主幹 (10354987)
Research Collaborator FUJII Kentaro  日本原子力研究開発機構, 先端基礎研究センター, 研究副主幹
SAITOH Yuji  日本原子力研究開発機構, 放射光科学研究ユニット, 研究副主幹
FUKUDA Yoshihiro  日本原子力研究開発機構, 放射光科学研究ユニット, 技術職員
SHIMADA Hiroyuki  東京農工大学, 大学院・工学研究院, 助教
SUMIYA Ryosuke  東京農工大学, 大学院・工学府, 博士前期課程
YASUHIRO Satoshi  東京農工大学, 大学院・工学府, 博士前期課程
FUKAO Taishi  東京農工大学, 大学院・工学府, 博士前期課程
MINAMI Hirotake  東京農工大学, 大学院・工学府, 博士前期課程
Project Period (FY) 2009 – 2011
KeywordsDNA放射線損傷 / 化学的損傷修復 / シンクロトロン放射光電子分光 / 電子エネルギー損失分光 / 時間相関分光 / 液体分子線 / 超励起分子のダイナミクス / 水素結合ネットワーク
Research Abstract

To investigate the radiation-induced damage on DNA and its self-assembled repair both as the alternative thermo-dynamical restoration processes of bio-cells, we have developed a novel spectroscopic method for damage in bio-molecules under mimetic cell condition by combining a liquid-micro-jet synchrotron-radiation photoelectron spectroscopy and a newly built high energy electron energy loss spectroscopy. To observed time-dependent appearance of anomalous molecular species following irradiation, a high resolution mass spectrometry has also been developed.

  • Research Products

    (48 results)

All 2012 2011 2010 2009

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (29 results) Book (3 results)

  • [Journal Article] Lifetime of the unpaired electron species in calf thymus DNA thin films induced by nitrogen and oxygen K-shell photoabsorption2012

    • Author(s)
      T. Oka, A. Yokoya, and K. Fujii
    • Journal Title

      Int. J. Radiat. Biol

    • Peer Reviewed
  • [Journal Article] Yield of single-, double-strandbreaks and nucleobase lesions in fully hydratedplasmid DNA films irradiated with high-LETcharged particles2012

    • Author(s)
      T. Ushigome, N. Shikazono, K. Fujii, R. Watanabe, M. Suzuki, C. Tsuruoka, H. Tauchi, and A. Yokoya
    • Journal Title

      Yield of single-, double-strandbreaks and nucleobase lesions in fully hydratedplasmid DNA films irradiated with high-LETcharged particles

    • Peer Reviewed
  • [Journal Article] The mutagenic potential of 8-oxoG/single strand break-containing clusters depends on their relative positions2012

    • Author(s)
      M. Noguchi, A. Urushibara, A. Yokoya, P. O' Neill, and N. Shikazono
    • Journal Title

      Mutat. Res

      Volume: 732 Pages: 34-42

    • Peer Reviewed
  • [Journal Article] Electron para-magnetic resonance study of unpaired electron species in thin films of pyrimidine bases in-duced by nitrogen and oxygen K-shell photo-absorption2011

    • Author(s)
      T. Oka, A. Yokoya, and K. Fujii
    • Journal Title

      Appl. Phys. Lett

      Volume: 98 Pages: 1-3

    • Peer Reviewed
  • [Journal Article] A novel technique using DNA denaturation to detect multiply induced single-strand breaks in a hydrated plasmid DNA molecule by X-ray and^4He^<2+> ion irradi-ation2011

    • Author(s)
      A. Yokoya, N. Shikazono, K. Fujii, M. Noguchi, and A. Urushibara
    • Journal Title

      Radiat. Protect. Dosim

      Volume: 143 Pages: 219-255

    • Peer Reviewed
  • [Journal Article] A model for analysis of the yield and the level of clustering of radia-tion-induced DNA strand breaks in hydrated plasmids2011

    • Author(s)
      N. Shikazono, A. Yokoya, A. Urushibara, M. Noguchi, and K. Fujii
    • Journal Title

      Radiat. Protect. Dosim

      Volume: 143 Pages: 181-185

    • Peer Reviewed
  • [Journal Article] Protective effect of silybin and analogues against X-ray radiation-induced damage2010

    • Author(s)
      H. Fu, M. Lin, Y. Muroya, Y. Katsumura, A. Yokoya, K. Hata, K. Fujii, and N. Shikazono
    • Journal Title

      Acta Biochim. Biophys. Sin

      Volume: 42 Pages: 485-495

    • Peer Reviewed
  • [Journal Article] X-ray absorption spectra for nucleotides(AMP, GMP, and CMP) in liquid water solutions near the nitrogen K-edge2010

    • Author(s)
      M. Ukai, A. Yokoya, K. Fujii, and Y. Saitoh
    • Journal Title

      Chem. Phys. Let

      Volume: 495 Pages: 90-95

    • Peer Reviewed
  • [Journal Article] Nucleo-base lesions and strand breaks in dry DNA thin film selectively induced by monochromatic soft X-rays2009

    • Author(s)
      K. Fujii, N. Shikazono, and A. Yokoya
    • Journal Title

      J. Phys. Chem. B

      Volume: 113 Pages: 16007-16015

    • Peer Reviewed
  • [Journal Article] Free radical scavenging reactions and antioxidant activities of silybin : mechanistic aspects and pulse radiolytic studies2009

    • Author(s)
      H. Fu, M. Lin, Y. Muroya, K. Hata, Y. Katsumura, A. Yokoya, N. Shikazono, and Y. Hatano
    • Journal Title

      Free Radic. Res

      Volume: 43 Pages: 887-897

    • Peer Reviewed
  • [Journal Article] Free radical scavenging and radioprotective effects of carnosine and anserine2009

    • Author(s)
      H. Fu, Y. Katsumura, M. Lin, Y. Muroya, K. Hata, K. Fujii, A. Yokoya, and Y. Hatano
    • Journal Title

      Radiat, Phys. Chem

      Volume: 78 Pages: 1192-1197

    • Peer Reviewed
  • [Journal Article] Induction of DNA strand breaks, base lesions and clustered dam-age sites in hydrated plasmid DNA films by ultrasoft X-rays around the phosphorus K-edge2009

    • Author(s)
      A. Yokoya, S. M. T. Cunniffe, R. Watanabe, K. Kobayashi, and P. O' Neill
    • Journal Title

      Radiat. Res

      Volume: 172 Pages: 296-365

    • Peer Reviewed
  • [Journal Article] Spectral change in X-ray absorption near edge structure of DNA thin films irradiated with monochromatic soft X-rays2009

    • Author(s)
      K. Fujii and A. Yokoya
    • Journal Title

      Radiat. Phys. Chem

      Volume: 78 Pages: 1188-1191

    • Peer Reviewed
  • [Journal Article] Synchrotron radiation photoelectron studies for primary radiation effects using a liquid water jet in vacuum : total and partial photoelectron yields for liquid water near the oxygen K-edge2009

    • Author(s)
      M. Ukai, A. Yokoya, Y. Nonaka, K. Fujii, and Y. Saitoh
    • Journal Title

      Radiat. Phys. Chem

      Volume: 78 Pages: 1202-1206

    • Peer Reviewed
  • [Journal Article] EPR study of radiation damage to DNA irradiated with synchrotron soft X-rays around nitrogen and oxygen K-edge2009

    • Author(s)
      A. Yokoya, K. Fujii, Y. Fukuda, and M. Ukai
    • Journal Title

      Radiat. Phys

      Volume: 78 Pages: 1211-1215

    • Peer Reviewed
  • [Journal Article] The yield, processing, and biological consequences of clustered DNA2009

    • Author(s)
      N. Shikazono, M. Noguchi, K. Fujii, A. Urushibara, and A. Yokoya
    • Journal Title

      J. Radiat. Res

      Volume: 50 Pages: 27-36

    • Peer Reviewed
  • [Presentation] 放射線によるゲノムDNA損傷の初期過程と生体修復2012

    • Author(s)
      横谷明徳
    • Organizer
      日本物理学会第67回年次大会
    • Place of Presentation
      関西学院大,兵庫県
    • Year and Date
      20120324-27
  • [Presentation] Unpaired electron species induced in DNA thin films during monochoromatic soft X-ray irradiation2012

    • Author(s)
      T. Oka, A. Yokoya, K. Fujii, Y. Fukuda, and M. Ukai
    • Organizer
      XII International Workshop on Radiation Damage to DNA
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2012-06-02
  • [Presentation] Variation in DNA damage induced by mono-chromatic soft X-rays in the energy region of oxygen K-edge2012

    • Author(s)
      Y. Sugaya, T. Shiina, I. Shiraishi, and A. Yokoya
    • Organizer
      XII International Workshop on Radiation Damage to DNA
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2012-06-02
  • [Presentation] Order effects of base excision processes to repair clustered DNA damage2012

    • Author(s)
      I. Shiraishi, T. Shiina, Y. Sugaya, N. Shikazono, and A. Yokoya
    • Organizer
      XII International Workshop on Radiation Damage to DNA
    • Place of Presentation
      Czech Republic
    • Year and Date
      2012-06-02
  • [Presentation] DNA damage caused by UV-A irradiation induces genetic instabilit2012

    • Author(s)
      A. Urushibara, S. Kodama, and A. Yokoya
    • Organizer
      XII International Workshop on Radiation Damage to DNA
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2012-06-02
  • [Presentation] Simula-tion study of microdosimetry and distribution of DNA damage inducing base damage2012

    • Author(s)
      R. Watanabe, T. Shiina, and A. Yokoya
    • Organizer
      XII International Workshop on Radiation Damage to DNA
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2012-06-02
  • [Presentation] Introduction to synchrotron radia-tion and radiobiological studies, Surface2011

    • Author(s)
      A. Yokoya
    • Organizer
      Electro, Radiation, and Photo Chemistry International Master Course(SERP-Chem), EU Education & Training ERASUMUS MUNDUS
    • Place of Presentation
      Orsay, France
    • Year and Date
      20111011-26
  • [Presentation] Order effect of base excision processes to repair clustered DNA damage2011

    • Author(s)
      A. Yokoya I. Shiraishi, and N. Shikazono
    • Organizer
      14th International Congress of Radiation Research
    • Place of Presentation
      Warszawa, Poland
    • Year and Date
      20110828-0901
  • [Presentation] AP sites in plasmind DNA caused by Auger effect of oxygen2011

    • Author(s)
      A. Yokoya, Y. Sugaya, K. Fujii, T. Oka, R. Watanabe, T. Kai
    • Organizer
      7th Inter-national Symposium on Physical, Molecular, Cellular, and Medical Aspects of Auger Processes
    • Place of Presentation
      Julich, Germany
    • Year and Date
      20110824-26
  • [Presentation] X-ray absorption spectrosco pic analysis of DNA damage induced by soft X-rays2011

    • Author(s)
      K. Fujii and A. Yokoya
    • Organizer
      7th International Symposium on Physical, Molecular, Cellular, and Medical Aspects of Auger Processes
    • Place of Presentation
      Julich, Germany
    • Year and Date
      20110824-26
  • [Presentation] Study of unpaired electron species in DNA film induced by nitrogen and oxygen K-shell photoabsorp-tion2011

    • Author(s)
      T. Oka, A. Yokoya, and K. Fujii
    • Organizer
      7th International Symposium on Physical, Molecular, Cellular, and Medical Aspects of Auger Processes
    • Place of Presentation
      Julich, Germany
    • Year and Date
      20110824-26
  • [Presentation] Near Edge X-ray Absorption spectroscopy of DNA damage induced by soft X-rays2011

    • Author(s)
      K. Fujii and A. Yokoya
    • Organizer
      14th International Congress of Radiation Research
    • Place of Presentation
      Warszawa, Poland
    • Year and Date
      2011-08-28
  • [Presentation] EPR study of unpaired electron species in DNA film induced by nitrogen and oxygen K-shell photoabsorp-tion2011

    • Author(s)
      T. Oka, A. Yokoya, and K. Fujii
    • Organizer
      14th International Congress of Radiation Research
    • Place of Presentation
      Warszawa, Poland
    • Year and Date
      2011-08-28
  • [Presentation] Significance of the repair synthesis in determining the biological consequences of clustered DNA damage2011

    • Author(s)
      N. Shikazono, M. Noguchi, A. Urishibara, P. O' Neill, and A. Yokoya
    • Organizer
      14th International Congress of Radiation Research
    • Place of Presentation
      Warszawa, Poland
    • Year and Date
      2011-08-28
  • [Presentation] Dependence of the yields of AP sites and AP clustered produced in plasmid DNA on scavenging capacity and LET2011

    • Author(s)
      T. Shiina, Y. Sugaya, I. Shiraishi, R. Watanabe, M. Suzuki, and A. Yokoya
    • Organizer
      XII International Workshop on Radiation Damage to DNA
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2011-06-02
  • [Presentation] Measurement of unpaired electron species in dry DNA thin films irradiated with synchrotron soft X-rays around oxygen and nitrogen K-edge studied by EPR2010

    • Author(s)
      T. Oka, A. Yokoya, K. Fujii, Y. Fukuda, and M. Ukai
    • Organizer
      2010 Inter-national Chemical Congress of Pacific Basin Societies(Pacifichem 2010)
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      20101215-20
  • [Presentation] Various modes of DNA damage formation by the direct effect of radiation2010

    • Author(s)
      A. Yokoya
    • Organizer
      56th Annual Meeting of Radiation Research Society
    • Place of Presentation
      Hawaii, USA.
    • Year and Date
      20100925-29
  • [Presentation] A novel technique using DNA denaturation-annealing to detect multiply induced base lesions in plasmid DNA molecules by X-ray irradiation2010

    • Author(s)
      A. Yokoya, N. Shikazono, and K. Fujii
    • Organizer
      11th International Workshop on Radiation Damage to DNA
    • Place of Presentation
      Atlanta, USA
    • Year and Date
      20100515-19
  • [Presentation] 放射線の直接作用による様々なDNA損傷生成モード2010

    • Author(s)
      横谷明徳
    • Organizer
      大阪大学産業科学研究所平成22年度-第2回量子ビーム科学研究施設研究会
    • Place of Presentation
      大阪大学,大阪
    • Year and Date
      2010-11-30
  • [Presentation] Near Edge X-ray absorption fine structure of DNA thin film irradiated with soft X-rays2010

    • Author(s)
      K. Fujii and A. Yokoya
    • Organizer
      37th International Conference on Vacuum Ultraviolet and X-ray Physics
    • Place of Presentation
      Vancouver, Canada
    • Year and Date
      2010-11-16
  • [Presentation] 難修復性のDNA損傷に関する物理学的及び生物学的アプローチによる研究2010

    • Author(s)
      横谷明徳
    • Organizer
      東北大学加齢研究所研究員会セミナー
    • Place of Presentation
      東北大学,仙台
    • Year and Date
      2010-08-11
  • [Presentation] シンクロトロン放射を用いたDNA損傷の分光研究2010

    • Author(s)
      横谷明徳
    • Organizer
      H22年度第1回東京RBC(放射線生物談話会)セミナー
    • Place of Presentation
      東京大学,東京
    • Year and Date
      2010-07-17
  • [Presentation] Soft X-ray absorption spectra of AMP and ATP in solutions2010

    • Author(s)
      H. Shimada, T. Fukao, H. Minami, M. Ukai, A. Yokoya, K. Fujii, Y. Fukuda, and Y. Saitoh
    • Organizer
      XII International Workshop on Radia-tion Damage to DNA
    • Place of Presentation
      Prague, Czech Republic
    • Year and Date
      2010-06-02
  • [Presentation] Mutagenic potential of clustered DNA damage containing single strand break and 8-oxoGs2009

    • Author(s)
      M. Noguchi, A. Urushibara, A, Yokoya, P. O' Neill, and N. Shikazono
    • Organizer
      15th International Symposium on Microdosimetry : An Internation-al Meeting on Ionizing Radiation Quality
    • Year and Date
      20091025-30
  • [Presentation] Analysis on the yield and the level of clustering of radiation-induced DNA strand breaks in hydrated plasmids2009

    • Author(s)
      N. Shikazono, A. Yokoya, A. Urushibara, M. Noguchi, and K. Fujii
    • Organizer
      15th International Symposium on Microdosimetry : An International Meeting on Ionizing Radiation Quality
    • Place of Presentation
      Verona, Italy
    • Year and Date
      20091025-30
  • [Presentation] A novel technique using DNA denature-tion to detect multiply induced single-strand breaks in a hydrated plasmid DNA molecule by X-ray and He^<2+> ion irradia-tion2009

    • Author(s)
      A. Yokoya, N. Shikazono, A. Urushibara, M. Noguchi, and K. Fujii
    • Organizer
      15th International Symposium on Micro-dosimetry : An International Meeting on Ionizing Radiation Quality
    • Place of Presentation
      Verona, Italy
    • Year and Date
      20091025-30
  • [Presentation] Synchrotron radiation photoelectron studies for primary radiation effects in bio-solutions : two dimen-sional electron spectra for liquid water in the soft X-ray region2009

    • Author(s)
      M. Ukai, Y. Nonaka, R. Sumiya, S. Yasuhiro, A. Yokoya, K. Fujii, and Y. Saitoh
    • Organizer
      11th International Coference on Electronic Spectroscopy and Structur
    • Place of Presentation
      Nara, Japan
    • Year and Date
      20091006-10
  • [Presentation] Near Edge X-ray absorption fine structure of DNA thin film irradiated with soft X-rays2009

    • Author(s)
      K. Fujii and A. Yokoya
    • Organizer
      11th International Coference on Electronic Spectroscopy and Structure
    • Place of Presentation
      Nara, Japan
    • Year and Date
      20091006-10
  • [Presentation] 生体溶液試料に対する二次イオン質量分析法の開発2009

    • Author(s)
      住谷亮輔,安廣哲,野中裕介,鵜飼正敏,横谷明徳,藤井健太郎
    • Organizer
      原子衝突研究協会第34回年会
    • Place of Presentation
      首都大学東京,東京
    • Year and Date
      20090828-30
  • [Book] Spectroscopic study of radiation-induced DNA lesions and their susceptibility to enzymatic repair2011

    • Author(s)
      A. Yokoya, K. Fujii, N. Shikazono, and M. Ukai
    • Total Pages
      543-574
    • Publisher
      CRC/Taylor & Francis Group
  • [Book] 放射線作用の基礎過程2011

    • Author(s)
      鵜飼正敏
    • Total Pages
      455-474
    • Publisher
      朝倉書店
  • [Book] ペニングイオン化2011

    • Author(s)
      鵜飼正敏
    • Total Pages
      41-59
    • Publisher
      国際文献印刷社

URL: 

Published: 2013-07-31  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi